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Population genetic structure of Fusarium verticillioides causal agent of rice crown and root rot in Ilam province using SSR Marker (Article in English) | ||
| آفات و بیماری های گیاهی | ||
| Article 20, Volume 83, No. 2 - Serial Number 101, March 2016, Pages 13-22 PDF (240 K) | ||
| Document Type: Plant Pathology | ||
| DOI: 10.22092/jaep.2016.106126 | ||
| Authors | ||
| KH. NOUROLLAHI* ; Z. HAGHI | ||
| Abstract | ||
| Study on the genetic structure of Fusarium verticillioides populations, provides different levels of information in management of root rot disease in rice farms.Simple Sequence repeat (SSR) markers were used to determine genetic structure and genetic diversity in 39 F. verticillioides isolates from five different regions in Ilam province, such as: Tangehghir, Lomar, Shirvan, Sarableh and Darehshahr. Average number of alleles in populations were 10.6, the number of alleles in populations varied from 9 allele in Tangehghir and Darehshahr as the lowest to 13 allels in Lomaras the highest. Observed number of alleles (Na) and effective number of alleles (Ne) were higher in Lomar (Na = 1; Ne = 1.276) compared to other populations. The genetic diversity (H)and Shannon's Information index (I) were also higher in Lomar (H = 0.167; I = 0.254) but lower values were estimated for Darehshahr (He = 0.141; I = 0.204). The lowest genetic distance was found between Tangehghir and Lowmar (0.00) and then Shirvan, while the highest genetic distance was revealed between Darehshahr and Tangehghir (0.018). Total gene diversity (Ht) and gene diversities between subpopulations (Hs) were estimated 0.125 and 0.116, respectively. Gene diversity attributable to differentiation among populations (Gst) was 0.072, while gene flow (Nm) was 6.368. Cluster analysis based on UPGMA showed the lowest genetic distance between Tangehghir and Lomar and then between Shirvan. The dendrogram indicated a high genetic distance between Darehshahr and four remaining populations.Results from this study will be useful in developing necessary control methods for Rice crown and root rot disease. | ||
| Keywords | ||
| Fusarium verticillioides; Genetic structure; rice; SSR | ||
| References | ||
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AMOAH, B. K., MACDONALD, M. V., REZANOOR, H. N. and NICHOLSON, P. 1996. The use of the random amplified polymorphic DNA technique to identify mating groups in the Fusarium section Liseola. Plant Pathology, 45: 115-125.
AMOAH, B. K., REZANOOR, H. N., NICHOLSON, P. and MACDONALD, M. V. 1995. Variation in the Fusarium section Liseola. Pathogenicity and genetic studies of isolates of Fusarium moniliforme Sheldon from different host in Ghana. Plant Pathology, 44: 563-572.
BAYRAKTAR, H. 2010. Genetic diversity and population structure of Fusarium oxysporum f. sp. cepae, the Causal agent of Fusarium basal plate rot on Onion, using RAPD Markers. Journal of Agricultural Sciences, 16: 139-159.
BOOTH, C. 1971. The genus Fusarium. Common wealth Mycological Institute: Kew, Surrey, UK. 32-185.
BRITZ, H. C., COUTINHO, T. A., WINGFIELD, B. D. and WINGFIELD, M. J. 2002. Sequence characterized amplified polymorphic markers for the pitch canker pathogen, Fusarium circinatum. Molecular Ecology, 3: 577.
BURGESS, L. W., SUMMERELL, B. A., BACKHOUSE, D., BENYON, F. and LEVIC, J. 1996. Biopdiversity and population studies in Fusarium. Sydowia, 48: 1-11.
BURGESS, T., WING, M. and WING, B. 2001. Simple sequence repeat markers distinguish among morphotypes of Sphaeropsis sapinea. Applied environmental microbiology, 67: 354-362.
CHEN, Y., WANG, J. X., ZHOU, M. G., CHEN, C. J. and YUAN, S. K. 2007. Vegetative compatibility of Fusarium graminearum isolates and genetic study on their carbendazim resistance recombination in China. Phytopathology, 97: 1584-1589.
DARVISHNIA, M., ALIZADEH, A., ZAREA, R. and MOHAMMADI GOLTAPEH, E. 2007. Three new Fusarium taxa isolated from gramineous plants in Iran. Rostaniha, 7 (2): 193-205.
DATTA, S., CHOUDHARY, R. G., SHAMIM, M. D., SINGH, R. K. and DHAR, V. 2011. Molecular diversity in Indian isolates of Fusarium oxysporum f. sp. lentis inciting wilt disease in lentil (Lens culinaris Medik). African Journal of Biotechnology, 10: 7314-7323.
DESJARDINS, A. E., MANANDHAR, H. K., PLATTNER, R. D., MANANDHAR, G. G., POLING, S. M. and MARAGOS, C. M. 2000. Fusarium species from Nepalese rice and production of mycotoxins and gibberellic acid by selected species. Applied environmental microbiology, 66: 1020-1025.
DOYLE, J. J. and DOYLE, J. L. 1990. A rapid total DNA preparation procedure for fresh plant tissue. Focus, 12: 13.
EBADI, M., RIAHI, H. and ARE, R. 2013. Genetic diversity of Fusarium semitectum isolates from rice using RAPD and REP-PCR markers. Mycologia Iranica, 1: 14-20.
HSIEH, H. W., SMITH, S. N. and SNYDER, W. C. 1977. Mating groups in Fusarium moniliform. Phytopathology, 67: 1041-1043.
HUSS, M. J., CAMPBELL, C. L., JENNINGS, D. B. and LESLIE, J. F. 1996. Isozyme variation among biological species in the Gibberella fujikuroi species complex (Fusarium section Liseola). Applied and Environmental Microbiology, 62: 3750-3756.
JO, Y. K., CHANG, S. W., REES, J. and JUNG, G. 2008. Reassessment of vegetative compatibility of Sclerotinia homoeocarpa using nitrate-nonutilizing mutants. Phytopathology, 98: 108-114.
KELLER, S. M., MCDERMOTT, J. M., PETTWAY, R. E., WOLFE, M. S. and MCDONALD, B. A. 1997. Gene flow and sexual reproduction in the wheat glum blotch pathogen Phaeosphaeria nodorum (anamorph Stagonospora nodorum). Phytopathology, 87: 353-358.
KENDRICK, B. 2003. Analysis of morphogenesis in hyphomycetes: new characters derived from considering some conidiophores and conidia as condensed hyphal systems. Canadian Journal of Botany, 81: 75-100.
KOSMAN, E. and LEONARD, J. 2005. Similarity coefficients for molecular markers in studies of genetic relationships between individuals for haploid, diploid, and polyploid species. Molecular Ecology, 14: 415-424.
LESLIE, J. F. 1991. Mating populations in Gibberella fujikuroi (Fusarium section Liseola). Phytopathology, 81: 1058-1060.
LESLIE, J. F. 1993. Fungal vegetative compatibility. Annual Review of Phytopathology, 31: 127-151.
LESLIE, J. F. 1995. Gibberella fujikuroi: available populations and variable traits. Canadian Journal of Botany, 7: 282-291.
LESLIE, J. F. and SUMMERELL, B. A. 2006. The Fusarium Laboratory Manual. Blackwell. 388 p.
LESLIE, J. F., PEARSON, C. A., NELSON, P. A. and TOUSSOUN, T. A. 1990. Fusarium spp. From maize, sorghum and soybean fields in the central and eastern United States. Phytopathology, 86: 343-350.
LI, Y. C., KOROL, A. B., FAHIMA, T., BEILES, A. and NEVO, E. 2002. Microsatellites: genomic distribution, putative functions and mutational a review mechanisms. Molecular Ecology, 11: 2453-2465.
MCDERMOT, J. M. and MCDONALD, B. A. 1993. Gene flow in plant pathosystems. Annual Review of Phytopathology, 31: 353-373.
MCDONALD, B. A. 2004. Population genetics of plant pathogens. The plant health instructor, DOI, 10.1094/PHI-A-2004-0524-01.
MCDONALD, B. A. and MCDERMOTT, J. M. 1993. Population genetics of plant pathogenic fungi. Bioscience, 43: 311-319.
MITTER, N., SRIVASTAVA, A. C., AHMAD, R. S., SARBOY, A. K. and AGARWAL, D. K. 2001. Characterization of gibberellin producing strains of Fusarium moniliforme based on DNA polymorphism. Mycopathologia, 153: 187-193
NEI, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 76: 379.
NEI, M. 1973. Analysis of the genetic diversity in subdivided populations. Proceedings of the National Academy of Sciences, USA 70: 3321-3323.
NEI, M. and LI, W. H. 1979. Mathematical Model for Studying Genetic Variation in Terms of Restriction Endonucleases. Proceeding of the national academy of science U. S. A., 76:5269-5273.
NELSON, P. E., TOUSSOUN, T. A. and COOK, R. J. 1981. Fusarium, diseases, biology, taxonomy. The Pennsylvania State University Press. University Park, PA, USA.
NELSON, P. E., TOUSSOUN, T. A. and MARASAS, W. 1983. Fusarium species: An illustrated manual for identification. The Pennsylvania State University Press. University Park, PA, USA.
NIRENBERG, H. I. 1981. A simplified method for identifying Fusarium spp. occurring on wheat. Canadian Journal of Botany, 59: 1599-1609.
NYVALL, R. F. 1999. Field crop diseases. Iowa State University Press, USA. 1021 p.
ODONNELL, K., CIGENLINK, E. and NIRENBERG, H. I. 2000. Molecular systematic and phylogeography of the Gibberella fujikuroi species complex. Mycologia, 90: 467-493.
PEAKALL, R. and SMOUSE, P. E. 2012. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics, 28: 2537.
REN, X., ZHU, Z. D., LI, H. J., DUAN, C. X. and WANG, X. M. 2012. SSR marker development and analysis of genetic diversity of Fusarium verticillioides isolated from maize in China. Scientia Agriculture Sinica, 45: 52-66.
ROLDAN-RUIZ, I., DENDAUW, J. E., VAN BOCKSTAELE, E., DEPICKER, A. and LOOSE, M. 2000. AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Molecular Breeding, 6: 125-126.
ROLHF, F. J. 1990. NTSYSPc, Numerical taxonomy and multivariant analysis system. Version 2.02. Applied Biostatistics. New York.
SAHARAN, M. S. and NAEF, A. 2008. Detection of genetic variation among Indian wheat head scab pathogens (Fusarium spp./isolates) with microsatellite markers. Crop Protection, 27: 1148-1154.
SAREMI, H. 2000. Plant diseases caused by Fusarium species. 1st Edn. Jehad Daneshgahi Press University of Mashhad, Iran, pp: 1145.
SHAH, D., BERGSTROM, G. C. and UENG, P. P. 1995. Initiation of Septoria nodorum blotch epidemics in winter wheat by seed born Stagonospora nodorum. Phytopathology, 85: 452-457.
SLIPPERS, B., BURGESS, T., WINGFIELD, B. D., CROUS, P. W., COUTINHO, T. A. and WINGFIELD, M. J. 2004b. Development of simple sequence repeat markers for Botryosphaeria spp. with Fusicoccum anamorphs. Molecular ecology notes, 4: 675-677.
SNEATH, P. H. A. and SOKAL, P. R. 1973. Numerical taxonomy. San Fransisco: W. H. Freeman and Company.
TAHERKHANI, K., ALIZADEH, A., FAROKHINEJAD, R. and SHARIFITEHRANI, A. 1998. Identification of causal agents of sugarcane Fusarium diseases in Khuzestan Province. In: Proceedings 13th Iranian plant protection congress, Karaj, Iran. 23-27 Aug., Karaj, Iran: 120.
TENZER, I., IVANISSEVICH, S. D., MORGANTE, M. and GESSLER, C.1999. Identification of microsatellite markers and their application to population genetics of Venturia inaequalis. Phytopathology,89: 748-753.
VARSHNEY, R. K., CHABANE, K., HENDRE, P. S., AGGARWAL, R. K. and GRANER, A. 2007. Comparative assessment of EST-SSR.; EST-SNP and AFLP markers for evaluation of genetic diversity and conservation of genetic resources using wild. cultivated and elite barleys. Plant Science, 173: 638-649.
VOIGT, K., SCHLEIER, S. and BRUCKNER, B. 1995. Genetic variability in Gibberella fujikuroi and some related species of the genus Fusarium based on random amplification of polymorphic DNA (RAPD). Current Genetics, 27: 528-535.
WEISING, K., KAEMMER, D., EPPLEN, J. T., WEIGAND, F., SAXENA, M. and KAHL, G. 1991. DNA fingerprinting of Ascochyta rabiei with synthetic oligodeoxynucleotides. Current Genetics, 19: 483-489.
WHITE, D. G. 1999. Compendium of Corn diseases. 3rd Ed. The American Psychopathological Association. 78 pages.
WOSTEMEYER, J. and KREIBICH, A. 2002. Repetitive DNA elements in fungi (Mycota): impact on genomic architecture and evolution. Current Genetics, 41: 189-198.
XU, J. R. and LESLIE, J. F. 1996. A genetic map of Gibberella fujikuroi mating population A (Fusarium moniliforme). Genetics, 143: 175-189.
YEH, F. C., YANG, R. C. and BOYLE, T. 1999. Microsoft Window-based freeware for population genetic analysis (POPGENE, ver. 1.31), ftp://ftp.microsoft.com /softlib/mslfiles/hpgl.exe. | ||
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