Zolfagharian, H., Mohajeri, M., Babaie, M., Mosavari, N. (2017). Investigation of the antibacterial effect of crude venom of Honey bee (Apis mellifera) and its fractions with disk diffusion method. , 30(1), 117-126. doi: 10.22034/vj.2017.107803
Hossein Zolfagharian; Mohammad Mohajeri; Mahdi Babaie; Nader Mosavari. "Investigation of the antibacterial effect of crude venom of Honey bee (Apis mellifera) and its fractions with disk diffusion method". , 30, 1, 2017, 117-126. doi: 10.22034/vj.2017.107803
Zolfagharian, H., Mohajeri, M., Babaie, M., Mosavari, N. (2017). 'Investigation of the antibacterial effect of crude venom of Honey bee (Apis mellifera) and its fractions with disk diffusion method', , 30(1), pp. 117-126. doi: 10.22034/vj.2017.107803
Zolfagharian, H., Mohajeri, M., Babaie, M., Mosavari, N. Investigation of the antibacterial effect of crude venom of Honey bee (Apis mellifera) and its fractions with disk diffusion method. , 2017; 30(1): 117-126. doi: 10.22034/vj.2017.107803

Investigation of the antibacterial effect of crude venom of Honey bee (Apis mellifera) and its fractions with disk diffusion method

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 14, Volume 30, Issue 1, March 2017, Pages 117-126    PDF (1.71 M)
Document Type: Full Research Paper
DOI: 10.22034/vj.2017.107803
Authors
Hossein Zolfagharian* 1; Mohammad Mohajeri2; Mahdi Babaie3; Nader Mosavari4
1Department of Venomous Animals and Antivenom Production, Razi Vaccine and Serum Research Institute, Karaj, Iran.
2Department of Toxicology, Faculty of Pharmacy, Islamic Azad University, Shahreza Branch, Isfahan, Iran.
3Young Researchers and Elites club, Science and Research Branch, Islamic Azad University, Tehran, Iran.
4Bovine tuberculosis reference laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.
Abstract
In the world of pharmacology, along with the discovery of new antibiotics, bacteria also gain features that antibiotics are ineffective against, and the issue of bacterial resistance in such a situation arises. Venom of animals have anti-bacterial effects among which, honey bee venom has therapeutic effects including anti-cancer, anti-arthritic and anti-inflammatory effects. The aim of this study was to evaluate the effects of crude venom of honey bee and its fractions on bacteria. In this study, the antibacterial activity of honey bee venom (Apis mellifera) and its fractions against five bacterial species including Escherichia coli, Salmonella typhyimurium, Pseudomonas aeruginosa, Burkholderia mallei and Burkholderia pseudo mallei was investigated. In this regard, different volumes of crude venom and two fractions which were obtained by gel filtration with standard antibiotic as positive controls by disc-diffusion method were evaluated and the inhibition zone was measured. The results showed that crude venom of honey bee and its fractions have a positive effect on Escherichia coli and Salmonella typhimurium. The inhibition zone around the disc in concentrations of 25, 35 and 45 µl for Escherichia coli was about 7, 10 and 14 mm and for Salmonella typhimurium was about 7, 9 and 12, respectively. This venom and its fractions had no effect on the other tested bacteria. Statistical analysis showed that p-value was less than 0.05. Analysis of the venom volume and the test samples proved that increasing the venom volume leads to a relative increase in anti-bacterial effect.
Keywords
bee venom; Antibacterial; disk diffusion; Escherichia coli; Salmonella typhyimurium
References


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