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Immunohistochemical Localization of Ghrelin in Goat Testicular Tissue | ||
| تحقیقات دامپزشکی و فرآوردههای بیولوژیک | ||
| Article 22, Volume 30, Issue 2, May 2017, Pages 185-193 PDF (795.04 K) | ||
| Document Type: Full Research Paper | ||
| DOI: 10.22034/vj.2017.109238 | ||
| Authors | ||
| Keifsan Mansouri1; Borhan Shokrollahi* 2 | ||
| 1MSc of Animal Physiology, Islamic Azad University, Sanandaj Branch, Kurdestan, Iran | ||
| 2Assistant Professor, Department of Animal Sciences, Islamic Azad University, Sanandaj Branch, Kurdestan, Iran. | ||
| Abstract | ||
| The aim of the present study was to investigate the immunohistochemical localization of ghrelin in testicular tissues of male goat. Ghrelin, a 28-amino acid acylated peptide, has been identified as an endogenous ligand for GH secretagogue receptor and the role of ghrelin in reproduction has been recently established. The immunohistochemical localization of ghrelin in male goat testicular tissues was performed using mouse monoclonal anti-ghrelin antibody as primary antibody and donkey anti-rabbit IgG (HRP) Polyclonal antibody as secondary antibody. Samples of testis were collected from three male goats (1.5 to 2-years old), and were kept in 10% Formalin for IHC test. Then paraffin blocks and histological sections were prepared. Immunoreactions were observed in spermatogenesis process, Leydig and sertoli cells. It is believed that the site of ghrelin expression in stages of spermatogenesis, Leydig and sertoli cells may indicate its role in local regulations. This is one of the first studies to provide molecular evidence for the presence of ghrelin within the entire testicular tissues of male goat. | ||
| Keywords | ||
| Ghrelin; leydig cell; sertoli cell; testis; male goat | ||
| References | ||
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1. Barreiro, M.L., Gaytan, F. Caminos, J.E. Pinilla, L. Casanueva, F.F. Aguilar, E. Dieguez, C. (2002). Cellular Location and Hormonal Regulation of Ghrelin Expression in Rat Testis. Journal. Biology of Reproduction.Vol, 67.pp: 1768–1776. 2. Barreiro, M.L., Tena-Semper, M.T. (2004). Ghrelin and reproduction: a novel signal linking energy status and fertility? Molecular and Cellular Endocrinology.Vol, 226.pp: 1–9. 3.Falken, Y. (2013). Effect of Endogenous and Exogenous Ghrelin on Gastrointestinal Function in Rat and Human. PhD thesis, department of clinical sciences, Karolinska Institute, Stockholm, Sweden. pp:1-60. 4.Ishikawa, T., Hitoshi, F. Takeshi, I. Atsushi, T. Masato, F. (2007). Ghrelin Expression in Human Testis and Serum Testosterone Level.Journal of Andrology. Vol. 28.No. 2. pp:320-4 5.Kheradmand, A., Omid, D. Masoud, A. Bahram, R. (2012). Ghrelin modulates testicular germ cells apoptosis and proliferation in adult normal rats. Biochemical and Biophysical Research Communications Vol. 419.pp: 299–304. 6.Lukasyk, A., Ludmila, R., Piotr, S., Aldona, K., Karolina, O., Marcin, Rucinski.,Marek, R., Jerzy, S. (2009). Immunohistochemicaland hybridocytochemical study on ghrelin signalling in the rat seminiferous epithelium. Journal of Folia Histochemica Et Cytobiologica. Vol. 47, No. 3.pp: 5-423. 7.Marie-Felix, A. (2010). Circulating Ghrelin Concentrations During the Transition Period of Dairy Cattle and the Associated Relationship with Milk Production. Thesis, Veterinary Science, the university of Arizona. 8.Miller, D.W., Joanne, L.H., Yvonne, A.B., Una, D., Alanna, L., Clare, L.A., Richard, G.L. (2005). Immunohistochemical evidence for an endocrine/paracrine role forghrelin in the reproductive tissues of sheep. Journal of Reproductive Biology and Endocrinology Vol. 3, No. 60. pp:1-14. 9.Moretti, E., Vindigni, C., Tripodi, S.A., Mazzi, L., Nuti, R., Figura, N., Collodel, G. (2013). Immunolocalisation of ghrelin and obestatin in human testis, seminal vesicles, prostate and spermatozoa. Andrologia. pp. 1–7. 11.Sato, T., Nakamura, Y. Shimura, Y. Ohgusu, H. Kangawa, K. Kojima, M. (2011). Structure, regulation and function of ghrelin.Journal of Biochemistry.Vol. 151.No. 2.pp: 119-128. 12.Tena-Sempere, M. (2008). Ghrelin and reproduction: ghrelin as novel regulator of the gonadotropic axis. Vitamins and Hormones.Vol. 77.pp: 285-300. 13.Yakan, B. (2011). A Concise Review: Ghrelin and Reproductive System. Erciyes Medical Vol. 33, No.4. pp:317-320. | ||
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