Saadati, R., Bahramnejad, B., Harighi, B. (2017). Characterization of rhizobial bacteria isolated from arsenic-contaminated site in south-eastern Kurdistan province and their influence on plant growth. , 5(1), 15-27. doi: 10.22092/sbj.2017.113117
R. Saadati; B. Bahramnejad; B. Harighi. "Characterization of rhizobial bacteria isolated from arsenic-contaminated site in south-eastern Kurdistan province and their influence on plant growth". , 5, 1, 2017, 15-27. doi: 10.22092/sbj.2017.113117
Saadati, R., Bahramnejad, B., Harighi, B. (2017). 'Characterization of rhizobial bacteria isolated from arsenic-contaminated site in south-eastern Kurdistan province and their influence on plant growth', , 5(1), pp. 15-27. doi: 10.22092/sbj.2017.113117
Saadati, R., Bahramnejad, B., Harighi, B. Characterization of rhizobial bacteria isolated from arsenic-contaminated site in south-eastern Kurdistan province and their influence on plant growth. , 2017; 5(1): 15-27. doi: 10.22092/sbj.2017.113117

Characterization of rhizobial bacteria isolated from arsenic-contaminated site in south-eastern Kurdistan province and their influence on plant growth

زیست شناسی خاک
Article 3, Volume 5, Issue 1, September 2017, Pages 15-27    PDF (6.15 M)
DOI: 10.22092/sbj.2017.113117
Authors
R. Saadati1; B. Bahramnejad2; B. Harighi* 3
1Former MSc. Student of University of Kurdistan
2Associate professor, University of Kurdistan
3Associate Professor, University of Kurdistan
Abstract
Symbiotic bacteria associated with the root of legume plants were isolated from soil and the root nodules of alfalfa (Medicago sativaL.), chickpea (Cicer arietinum L.) and yellows alfalfa (Melilotus officinalis L.) grown in arsenic-contaminated areas in south eastern part of Kurdistan province, Iran. According to physiological and biochemical  properties, isolates were identified as the members of Rhizobium and Ensifer genus. The presence of arsenic resistant system known as ars system was confirmed by amplification using primers corresponding to arsC gene. Growth rate of strains in different concentrations of arsenate was investigated in YEMB medium supplemented with 100-400 mM arsenate. Alfalfa,s isolates (AB1, AB3), chickpea,s isolates (PA2, PC2), yellow alfalfa,s isolate (YA1) and standard strain (Sinorhizobium meliloti SM117) were tolerant to 350 mM arsenate and (AB1, PA2 and YA1) isolates were moderately capable to grow at 400 mM arsenate. Among the identified strains, AB1 and PA2, were selected for greenhouse experiments. In order to evaluate the effect of arsenic contamination on legume-rhizobia symbiosis and biomass production of alfalfa and chickpea plants, an experiment was carried out based on a completely randomized design with a factorial arrangement in three replications. Experiment factors consisted of, three levels of rhizobia inoculation (with and without inoculation of AB1 and PA2) and five levels of arsenic concentrations (0. 10, 50, 75 and 100 mgkg-1 soil) under greenhouse conditions for 8 weeks. Results obtained in this study indicated that the fresh weight and dry weight of alfalfa and chickpea (shoot and root) were decreased as the arsenic concentration of the soil was increased. The results also showed that fresh and dry shoot and root weight of alfalfa and chickpea were significantly higher in rhizobia-inoculated treatments compared to non-inoculated plants.
Keywords
Arsenic resistance; legumes; Rhizobia
References
  1. Carrasco, J.A., Armario, P., Pajuelo, E., Burgos, A., Caviedes, M.A., Lopez, R., Chamber, M.A., Palomares, A.J. 2005. Isolation and characterisation of symbiotically effective rhizobium resistant to arsenic and heavy metals after the toxic spill at the Aznalcollar pyrite mine. Soil Biology and Biochemistry 37:1131–1140.
  2. Broos, K., Uyttebroek, M., Mertens, J. and Smolders, E. 2004. A survey of symbiotic nitrogen fixation by white clover grown on metal contaminated soils. Soil Biology and Biochemistry 36: 633-640.
  3. Chaudri, A.M., McGrath, S.P. and Giller, K.E. 1992. Survival of the indigenous population of Rhizobium leguminosarum biovar trifolii in soil spiked with Cd, Zn, Cu and Ni salts. Soil Biology and Biochemistry 24:625-632.
  4. Chowdhury, T.R., Basu, G.K., Mandal, B.K.,  Biswas, B.K., Samanta, G., Chowdhury, U.K., Chanda, R.K., Lodh, D., Roy, S.L., Saha, K.C., Roy, S., Kabir, S., Quamruzaman, Q. and Chakraborti, D. 1999. Arsenic poisoning in Gandes Delta. Nature 401:545-546.
  5.  Giller, K.E., Witter, E. and Mcgrath, S. 1998. Toxicity of heavy metals to microorganisms and microbial processes in agricultural soils: a review. Soil Biology and Biochemistry 30:1389-1414.
  6. Hao, X., Taghavi, S., Xie, P., Orbach, M.J., Alwathnani, H.A., Rensing, C. and Wei, G. 2014. Phytoremediation of heavy and transition metals aided by Legume-Rhizobia symbiosis. International Journal of Phytoremediation 16: 179-202.
  7. Huang, A., Teplitski, M., Rathinasabapathi, B. and Ma, L. 2010. Characterization of arsenic-resistant bacteria from the rhizosphere of arsenic hyperaccumulator Pteris vittata. Canadian Journal of Microbiology 56:236-246.
  8. Ike, A., Sriprang, R., Ono, H., Murooka, Y. and Yamashita, M. 2007. Bioremediation of cadmium contaminated soil using symbiosis between leguminous plant and recombinant rhizobia with the MTL4 and the PCS genes. Chemosphere 66:1670-1676.
  9. Jack, C.N., Wang, J. and Shraim, A.A. 2003. Global health problem caused by arsenic from natural sources. Chemosphere 52:1353-1359.
  10. Jackson, C.R., Jackson, E.F., Dugas, S.L., Gamble, K. and Williams, S.E. 2003. Microbial transformations of arsenite and arsenate in natural environments. Recent Research Developments in Microbiology 7: 103-118.
  11. Jackson, C.R., Dugas, S.L. and Harrison, K.G. 2005.  Enumeration and characterization of arsenate-resistant bacteria in arsenic free soils. Soil Biology and Biochemistry 37:2319-2322.
  12. Lafuente, A., Perez-Palacios, P., Doukkali, B., Molina-Sanchez, M.D., Jimenez-Zurdo, J.I., Caviedes, M.A., Rodriguez-Liorente, I.D., Pajuelo, E. 2015. Unraveling the effect of arsenic on the model Medicago-Ensifer interaction: a transcriptomic meta-analysis. New Phytologist 205: 255-272.
  13. Ma, L.Q., Kumar, K.M., Tu, C., Zhang, W., Cai, Y. and Kennelly, E.D. 2001. A fern that hyperacumulates arsenic. Nature 409:579.
  14. Mandal, S.M., Pati, B.R., Das, A.K. and Ghosh, A.K. 2008. Characterization of a symbiotically effective Rhizobium resistant to arsenic: isolated from the root nodules of Vigna mungo (L.)  Hepper grown in an arsenic-contaminated field. Journal of General and Applied Microbiology 54:93-99.
  15. Mateos, L.M., Ordonez, E., Letek, M. and Gil, J.A. 2006. Corynebacterium glutamicum as a model bacterium for the bioremediation of arsenic. International Microbiology 9: 207-215.
  16.  Mosaferi, M., Yunesian, M.,  Mesdaghinia, A.R., Nadim, A., Naseri, S. and Mahvi, A.H. 2003. Occurrence of arsenic in Kurdistan Province of Iran. In BUET-UNU international symposium, international training network centre. Dhaka. Bangladesh, Tokyou.
  17. Mukhopadhyay, R., Rosen, B.P., Phung, L.T. and Silver, S. 2002. Microbial arsenic: from geocycles to genes and enzymes. FEMS Microbiological Review 26:311-325.
  18. Pajuelo, E., Rodrı´guez-Llorente, I.D., Dary, M. and Palomares, A.J. 2008. Toxic effects of arsenic on Sinorhizobium-Medicago sativa symbiotic interaction. Environmental Pollution 154:203-211.
  19. Reichman, S.M. 2007 The potential use of the legume–rhizobium symbiosis for the remediation of arsenic contaminated sites. Soil Biology and Biochemistry 39:2587–2593.
  20. Sa-Pereira, P., Rodrigues, M., Videira e Casatro, I. and Simoes, F. 2007. Identification of an arsenic resistance in mechanism rhizobial strains. World Journal of Microbiology and Biotechnology 23:1351-1356.
  21. Sarkar, A, Kazy, S.K. and Sar, P. 2013. Characterization of arsenic resistant bacteria from rich groundwater of West Bengal, India. Ecotoxicology 22:363-376.
  22. Schaad, NW,  Jones, J.B. and Chun, W. 2001. Laboratory guide for identification of plant pathogenic bacteria. 3nd edition. The American Phytopathological Society, St. Paul. Minnesota, USA.
  23. Silver, S. 1996. Bacterial resistances to toxic metal ions: a review. Gene 179:9–19.
  24. Sriprang, R., Hayashi, M., Yamashita, M., Ono, H., Saeki, K. and Murooka, Y. 2002. A novel bioremediation system for heavy metals using the symbiosis between leguminous plant and genetically engineered rhizobia. Journal of Biotechnology 99:279-293.
  25. Talano, M.A., Cejas, R.B., Gonzalez, P.S. and Agostini, E. 2012. Arsenic effect on the model crop symbiosis Bradyrhizobium-soybean. Plant Physiology and Biochemistry 63:8–14.
  26. Teng, Y., Wang, X., Li, L., Li, Z. and Luo, Y. 2015. Rhizobia and their bio-partners as novel drivers for functional remediation in contaminated soils. Frontiers in Plant Science 6:1-11.
  27. Vazquez, S., Esteban, E. and Carpena, R.O. 2008. Evolution of arsenate toxicity in nodulated white lupine in a long-term culture. Journal of Agricultural Food Chemistry 56:8580–8587.
  28.  Vincent, J.M. 1970. A manual for the practical study of the root nodule bacteria. Blackwell Scientific Publication, Oxford.
  29. Wang, Q., Xiong, D., Zhao, P., Yu, X., Tu, B. and Wang, G. 2011. Effect of applying an arsenic-resistant and plant growth-promoting rhizobacterium to enhance soil arsenic phytoremediation by Populus deltoids LH05-17. Journal of Applied Microbiology 111:1065-1074.  
  30. Yang, H.C., Cheng, J.J., Finan, T.M., Rosen, B.P. and Bhattacharjee, H. 2005 Novel pathway for arsenic detoxification in the legume symbiont Sinorhizobium meliloti. Journal of Bacteriology 187: 6991–6997.
  31. Zandsalimi, S., Karimi, N. andKohandel, A. 2011.  Arsenic in soil, vegetation and water of a contaminated region. International Journal of Environmental Science and Technology 8: 331-33.
Statistics
Article View: 1,429
PDF Download: 908


counter
Journal management system. designed by sinaweb