Tabaei-Aghdaei, S., Mirjani, L., emam, M., Assareh, M., Ghamari-Zare, A. (2007). Effects of genotype and growth regulators on callus induction in Rosa damascena Mill. , 15(3), 222-230. doi: 10.22092/ijrfpbgr.2007.114949
Seyed Reza Tabaei-Aghdaei; L. Mirjani; mitra emam; M.H. Assareh; A. Ghamari-Zare. "Effects of genotype and growth regulators on callus induction in Rosa damascena Mill". , 15, 3, 2007, 222-230. doi: 10.22092/ijrfpbgr.2007.114949
Tabaei-Aghdaei, S., Mirjani, L., emam, M., Assareh, M., Ghamari-Zare, A. (2007). 'Effects of genotype and growth regulators on callus induction in Rosa damascena Mill', , 15(3), pp. 222-230. doi: 10.22092/ijrfpbgr.2007.114949
Tabaei-Aghdaei, S., Mirjani, L., emam, M., Assareh, M., Ghamari-Zare, A. Effects of genotype and growth regulators on callus induction in Rosa damascena Mill. , 2007; 15(3): 222-230. doi: 10.22092/ijrfpbgr.2007.114949

Effects of genotype and growth regulators on callus induction in Rosa damascena Mill

تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران
Article 5, Volume 15, Issue 3 - Serial Number 29, November 2007, Pages 222-230    PDF (185.89 K)
Document Type: Research Paper
DOI: 10.22092/ijrfpbgr.2007.114949
Authors
Seyed Reza Tabaei-Aghdaei* 1; L. Mirjani2; mitra emam3; M.H. Assareh3; A. Ghamari-Zare3
1Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran, E-mail:tabaei@rifr-ac.ir
2Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran,
3Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran
Abstract
Rosa damascena Mill. as a most important aromatic plants is the best commercial species of the fragrant roses in Iran and many countries. To study callus induction and proliferation in Rosa damascena Mill., axillary buds of five genotypes collected from different parts of Iran was used as explants. These were cultured on MS medium supplemented with growth regulator(s) and solifiled with 0.7% (w/v) agar, at 25±1 °C. Different concentrations of 2,4-D (2, 3 ,4 ,5 ,6 mg/l), IBA (0.1 mg/l), NAA (5 ,10 mg/l), PCPA (3, 5 ,7 ,9 ,10 mg/l) Kinetin (0.1, 1 mg/l) and BAP(1, 2mg/l) were used. Genotypes showed significant differences (P<0.01) for callus induction, Also, highest callus induction was observed in genotypes of Kashan cultured on MS cultured medium contained 1 mg/l Kinetin and 5 mg/l PCPA. The results emphasized on genotype, kind and concentration of growth regulators as key factors in callogenensis and proliferation of Rosa damascena.
Keywords
Callus induction; explant; PCAP (p- choloro phenoxy acetic acid) and Rosa damascena Mill
References
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