Related Links
Protective Effect of Flavonoids, Against Red Blood Cell Hemolysis | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| Article 9, Volume 21, Issue 4 - Serial Number 30, February 2006, Pages 505-515 PDF (456.41 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/ijmapr.2006.115032 | ||
| Authors | ||
| S. Asgary* 1; Gh. Naderi2; N. Askari3 | ||
| 1Ph.D, Associate Professor of Pharmacognosy, Isfahan Cardiovascular Research Center, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| 2Ph.D, Associate Professor of Biochemistry, Isfahan Cardiovascular Research Center, Isfahan University of Medical Sciences | ||
| 3M.S of Microbiology, Isfahan Cardiovascular Research Center, Isfahan University of Medical Sciences | ||
| Abstract | ||
| Flavonoids which are polyphenolic substances are found in different vegetable and fruits and they have anti-oxidant properties. Epidemiological studies showed that flavonoids reduce the prevalence of cardiovascular disease. On the other hand, the usage of synthetic antioxidants have been limited because of their toxicity and, there are different researches to find better natural antioxidants. This survey investigates the effect of some pure flavonoids such as kaempferol, quercetin, morin and rutin on red blood cell (RBC) hemolysis and their -SH capacity as membrane protection indicator. The flavonoids solutions were prepared. Rate of RBC hemolysis and -SH capacity of cell membrane were determined by spectrophotometer. 2,2´azobis2 amidino propane dihydrechloride (AAPH) was used to induce RBC peroxidation. The effect of each flavonoids on RBC hemolysis was examined in 3 concentrations (0.15, 5, 10) μg/ml but for investigating the effect on -SH groups only the highest concentration (10 μg/ml) of each flavonoids were used. In all cases, the antioxidant activity was dose-dependent. Rutin showed the highest inhibitory effect on RBC hemolysis than other flavonoid, that was 42.5. Also kaempferol, rutin, morin protect -SH groups 6%, 23.3%, 26.4% respectively. Results showed that flavonoids and plants contain flavonoids can be used as natural antioxidants for treatment and prevention of diseases which their pathogenesis are lipid peroxidation. | ||
| Keywords | ||
| Antioxidant; rutin; kaempferol; Quercetin; morin; RBC; -SH capacity | ||
| References | ||
|
- عسگری، ص., 1382. تأثیرگیاهان بابونه، بومادران و زالزالک بر افزایش مقاومت گلبولهای قرمز و میزان SH در مقابل مواد اکسید کننده. فصلنامه گیاهان دارویی. Genj-Tao, L., Tie-Mei, Z., Bao-en, W. and Ya-Wen W., 1992. Protective action of seven natural phenolic compounds against peroxidative damage to biomembranes, Biochemical Pharmacology, 43(2): 147-152. - Grinberg, L.N., et al. 1994. Protective effects of rutin against hemoglobin oxidation, Biochem Pharma, 48(4): 643-9. - Haest, C.W.M., Plasa, G., Kamp, D. and Deuticke, B., 1978. Spectrin as a stabilizer of the phospholipid asymmetry in the human erythrocyte membrane, Biochim Biophys Acta, 509: 21-23. - Harinder, S.G., 1997. Antioxidant and disease prevention. CRC Press, Boca RatonNew York., 1-4, 12-22, 26, 32, 38, 45, 54, 67-68, 115-117, 172. - Herbert, J.M., et al. 1996. Ability of different flavonoids to inhibit the procoagulant activity of adherent human monocytes, J. Natur. Prod., 59(3): 273-276. - Hertog, M GI, et al. 1997. Antioxidant flavonoids and ischemic heart disease in a Welsh population of men: the Caerphilly study. Am J Clin Nutr 65: 1489-1494. - Hiroyukr, H., Takashi, S., Harumi, I., Hideyuki, D., Shizuko, K. and Yukiyoshi, T., 1996. Antiperoxidative components in Thymus vulgaris, Planta Medica, 62: 217-220. - Horaguchi, H., et al. 1996. Antioxidative components in Thymus vulgaris, Planta Medica, 62: 217-221. - Ishikawa T, et al. 1997. Effect of tea flavonoid supplementation on the susceptibility of low-density lipoprotein to oxidative modification, Am. J. Clin. Nutr., 66: 261-266. - Katan, M.B., Hollman, P.C.H., 1998. Dietary flavonoids and cardiovascular disease, Nutr. Metab. Cardiovase Dis., 8: 1-4. - Kitagawa, S., 1996. Inhibitory effects of catechol derivative on hydrophilic free radical initiator induced hemolysis on hemoglobin, Chem. Pharm. Bull., 44(5): 518-519. - Koga, T., Moro, K. and Terao, Y., 1998. Protective effect of a vitamin E analog phosphatidylchromanol, against oxidative hemolysis of human erythrocytes, Lipid, 3(6): 589-595. - Lowry, O.H., Rosebrough, N.J., Farr Al Randall, R.J., 1951. Protein measurement with the folin phenol reagent, Biol. Chem., 193: 265-275. - Melzig, M.E., 1996. Inhibition of adenosine deaminase activity of aortic endothelial cells by selected flavonoids, Planta Medica, 62: 20-21. - Sanchez de Rojas VR, et al. 1996. Isolation of vasodilatory active flavonoids from the traditional remedy Satureja obovata. Planta Med 62: 282-294. - Visioli, F., et al. 1997. Evaluating oxidation processes in relation to cardiovascular disease: a current review of oxidant/antioxidant methodology, Nutr Metab Cardiovasc, 7: 459-66. - Fiorani, M., Accorsi, A. and Cantoni, O., 2003. Human red blood cells as a natural flavonoid reservoir, Free Radic. Res., 37(12): 1331-8. - Allison, R.W., Lassen, E.D., Burkhard, M.J. and Lappin, M.R., 2000,.Effect of a bioflavonoid dietary supplement on acetaminophen induced oxidative injury to feline erythrcyto, J. Am .Vet. Med. Assoc., 217 (8): 1157-61. - Zon, C.G., Agar, N.S., Jones, G.L., 2001. Oxidative insult to human red blood cells induced by free radical AAPH and its inhibition by a commercial antioxidant mixture, Life Sci., 69(1): 75-86. | ||
|
Statistics Article View: 815 PDF Download: 423 |
||
Journal management system. designed by sinaweb