Related Links
Cytogenetic studies in alfalfa (Medicago sativa L.) somaclones following tissue culture | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| Article 7, Volume 13, Issue 2 - Serial Number 20, July 2006, Pages 209-220 PDF (1.21 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/ijrfpbgr.2005.115176 | ||
| Authors | ||
| Abbas Safarnejad* 1; S. Marvian2 | ||
| 1Faculty member of Khorasan Razavi Agricultural and Natural Resources Research Center or Mashhad Branch, Education and Extension Organization (AREEO) | ||
| 2Khorasan Agriculture and Natural Resources Research Center P.o Box 1148-91735, Mashhad, Iran. | ||
| Abstract | ||
| Alfalfa (Medicago sativa L.) grows extensively in farms and rangelands. In order to increase in vitro plant regeneration and salt tolerance in alfalfa (Medicago sativa L.), plants were regenerated from CUF101-1S genotype and seeds were produced. Chromosomes morphology was studied in three genotypes CUF101-1S, Reg1S and Reg2S of Medicago, using their root meristem after fixation, hydrolysis and staining stages. The result showed that all three genotypes were 2n=4x=32. Karyotypic formula was 8SM+ 7M + 1 AC in CUF101-1S and were 12 M + 4SM in Reg2S. Total chromosomes length in CUF101-1S was 31.6 and the longest and the shortest chromosomes lengths were 3.2 and 1.2, respectively. These values were stimated 31.2, 3, 19 and 25.9, 2.4, 16 for Reg1S and Reg2S, respectively. | ||
| Keywords | ||
| Medicago sativa L; cytogenetic; Tissue culture and Somaclone | ||
| References | ||
|
صفرنژاد، ع. 1382. استفاده از تنوع سوماکلونی برای سازگاری به تنش‌های محیطی در هیبرید گندم و جو. مجموعه مقالات سومین همایش ملی بیوتکنولوژی، جلد دوم: 340-342، مشهد. مرویان، ث، صفرنژاد، ع. و مجد، ا.، 1381. مقایسه کاریولوژی برخی ارقام یونجه. پژوهش و سازندگی، شماره 56 و57 :25-29. Ahloowalia, B.S., 1975. Regeneration of ryegrass plants in tissue culture. Crop Sci., 15: 449-452. Ahloowalia, B.S., 1976. Chromosome changes in parasexually produced ryegrass. In: Jones K. and P. Brandham (Eds.). Current Chromosome Research, North Holland, Amsterdam, pp. 115-122. Ahloowalia, B.S., 1983. Spectrum of variation in somaclones of triploid ryegrass. Crop Sci., 23: 1141-1147. Ahloowalia, B.S., 1986. Limitations to the use of somaclonal variation in crop improvement. In: J. Semal (Eds.), Somaclonal Variation and Crop Improvement, pp. 14-27. Martinus Nijhoff, Boston. Creissen, S.S., and Karp, A., 1985. Karyotypic changes in potato plants regenerated from protoplasts. Plant Cell Tiss. Org. Cult., 171-182. Duncan, R.R., 1997. Tissue culture-induced variation and crop improvement. Adv. Agron., 58: 201-240. Gupta, P.K., 1998. Chromosomal basis of somaclonal variation in plants. In: Jain, S.M., Brar, D.S. and Ahloowalia, B.S., (Eds.). Somaclonal Variation and Induced Mutations in Crop Improvement, Kluwer Academic Publishers, Dordrecht, pp. 149-168. Huziwara, Y., 1962. Karyotype analysis in some genera of Compositae. VIII. Further studies on the chromosome of Aster. Amer. J. Bot. 49: 116-119. Jain, S.M. 1997a. Somaclonal variation and mutagenesis for crop improvement. Maat-alouden tutkimuskeskuksen , Sirkka Immonen (Ed.), Vol. 18, pp. 122-132. Jain, S.M., 2001, Tissue culture-derived variation in crop improvement. Euphytica, 118: 153-166. Jain, S.M., Brar, D.S., and Ahloowalia, B.S., (Eds.) 1998. Somaclonal variation and induced mutations in crop improvement. Kluwer Academic Publishers, UK. Kaeppler, S.M., Phillips, R.L. and Olhoft, P., 1998. Molecular basis of heritable tissue culture-induced variation in plants. In: S.M. Jain, D.S. Brar and B.S. Ahloowalia (Eds.), Somaclonal Variation and Induced Mutations in Crop Improvement, Kluwer Academic Publishers, Dordrecht, pp. 467-486. Larkin, P.J. and Scowcroft, S.C., 1981. Somaclonal variation: a novel source of variability from cell cultures for plant improvement. Theoretical and Applied Genetics, 60: 197-214. Micke, A., 1999. Mutations in plant breeding. In: B.A. Siddiqui, S. Khan (Eds.), Breeding in Crop Plants-Mutations and in vitro Mutation Breeding, Kalyani Publishers, Nee Delhi, India, pp. 1-19. Roth, R., Ebert, I. and Schmidt, J., 1997. Trisomy associated with loss of mutation capacity in a long-term embryogenic cultures of Abies alba. Theor Appl Genet, 95: 353-358. Safarnejad, A., Collin, H.A., Bruce, K.D. and McNeilly, T., 1996. Characterization of alfalfa (Medicago sativa L.) following in vitro selection for salt tolerance. Euphytica, 92: 55-61. Skula, P. and Misra, S.P., 1994. An introduction to taxonomy of angiosperms. VIKS Publishing House Put LTD, New Delhi, 256 P.P. Stace, C.A. 1984. Plant taxonomy and biosystematics. Pitman press, Bath, 382 P.P. Veilleux, R.E. and Johnson, A.A.T., 1998. Somaclonal variation: Molecular analysis, transformation, interaction and utilization. Plant Breed. Rev.16: 229-268. | ||
|
Statistics Article View: 299 PDF Download: 295 |
||
Journal management system. designed by sinaweb