
Micropropagation of Populus caspica by internode culture | ||
تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
Article 4, Volume 9, Issue 1 - Serial Number 9, June 2002, Pages 113-122 PDF (317.19 K) | ||
DOI: 10.22092/ijrfpbgr.2002.115793 | ||
Authors | ||
M. Emam* ; A. Jafari-Mofidabadi | ||
Research Institute of Forests and Ranselands. P.O.Box 13185-116. Tehran | ||
Abstract | ||
Shoot tips of elite adult trees of Populus caspica in northern forest of Iran (Gillan, Safrabaste) were collected. After sterilization the materials, these explants were cultured in ½ MS medium with 0.5 mg / lit BA + 0.01 mg / lit IBA. Enough proliferated shoots were obtained. Internodes with length of 0.5 to 1 cm , were cultured in MS and ½ MS with 3 hormone treatments ( BA : 0.1, 0.5 and 1 mg/lit) with three replications. The differences of proliferation and shoot length growth between treatments were investigated with a factorial experiment and Duncan’s test. Results showed that the best medium for internodes’ micropropagation of P. caspica, was MS with 0.5 mg / lit BA. Rooting of shoots were obtained in ½ MS with IBA in 0.5 mg/lit. | ||
Keywords | ||
Internode culture; Vegatative propagation; Populus caspica | ||
References | ||
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