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Callus types and plant regeneration in Hordeum species | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| Article 2, Volume 5, Issue 1 - Serial Number 5, March 2002, Pages 17-35 PDF (730.39 K) | ||
| DOI: 10.22092/ijrfpbgr.2000.115888 | ||
| Author | ||
| seyed R. Tabaei-Aghdaei* | ||
| Department of Genetics and Plant Physiology, Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran | ||
| Abstract | ||
| Calli were induced from both mature and immature embryos of a wild barley species (Hordeum murinum) and of cultivated barley (H vulgare). Mature embryos produced soft watery and translucent cultures with the characteristics of non-embryogenic (NE) callus. Such cultures grew slowly and presented merely some root formation. Cultures derived from immature embryos showed the characteristics of NE calli primarily, most of which grew slowly. From these calli rapidly growing calli were produced in cultivated barley, characterized as being dry firm and granular or with small clumps and yellowish to white in color. Shoot-like structures emerged on these cultures and the shoot and root development occurred on regeneration medium. The well-developed planletes grew normally after transferring to soil and reached to maturity and seed production phase. | ||
| Keywords | ||
| Callus; regeneration; Immature embryos; Embryogenic; Non-embryogenic; Hordeum tnurinum; H. vulgare | ||
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