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Mapping QTLs associated with Barley seed germination under normal and drought stress conditions | ||
| علوم و فناوری بذر ایران | ||
| Article 17, Volume 8, Issue 1 - Serial Number 15, July 2019, Pages 227-239 PDF (713.06 K) | ||
| Document Type: Original Article | ||
| DOI: 10.22034/ijsst.2018.116582.1150 | ||
| Authors | ||
| zeinab taghizadeh* 1; hossein sabouri2; Hossein Hosseini, Moghaddam3; Fallahi Hossein Ali4; Mahnaz Katouzi5 | ||
| 1Msc student of biotecnology | ||
| 2Department of plant production, collage of agriculture science, Gonbad Kavous University | ||
| 3Assistant Professor, Department of Plant Production, Faculty of Agriculture and Natural Resources, Gonbad Kavous University | ||
| 4Assistant of Crop, Research Division, Research and Education Center of Agriculture and Natural Resourceso of Sari. | ||
| 5phD Student of nuclear Agriculture, Gorgan Agriculture Science and Natural Resources. | ||
| Abstract | ||
| Seed germination is a manifest characteristic of the standard germination test of a cultivated variety. In order to identify genomic regions associated with seed germination, 103 family of barley in F3 and their parents Badia and Kavir were cultivated in 2015-2016, under normal and drought stress conditions. Different traits including Plumule length, Radicle number, Cleoptil length, Radicle Dry weight, Plumule Dry weight and germination speed were measured. In this study, 28 pairs of microsatellite primers were used to determine the parental polymorphism. QTL analysis using composite interval mapping (CIM) for each trait in each environment for all studied traits. In general, twenty seven QTLs were detected for all traits. Total phenotypic variance explained by these QTLs varied from 8.6 to 37.3 percent. The highest LOD value for QTL controlling Radicle number (LOD=10.45) was obtained on chromosome 4 qNR-4a -15 in drought stress The results revealed that QTLs of Radicle number had desirable performance in all two environments. However, detected stable and cluster QTLs may be used in marker assisted selection (MAS) programs. | ||
| Keywords | ||
| Poly ethylene glycol; Radicle; SSR; Plumule | ||
| References | ||
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Abdolshahi, R., M. Omidi, A. Talei, and B. Yazdi Samadi, 2009. Mapping controller QTLs of drought stress in bread wheat (Triticum aestivum). J. Agric. Res. 7: 539-527. (In Persian, with English Abstract) Cattivelli, L., F. Rizza, F.W. Badeck, E. Mazzucotelli, A.M. Mastrangelo, E. Francia, C. Mare, A. Tondelli, and A.M. Santaca, 2008. Drought tolerance improvement in crop plants: an integrated view from breeding to genomics. Field Crops Res. 105: 1-14. Cheng, H.T., H. Jiang, X.U.E. Da-Wei, G.U.O. Long-Biao, Z.E.N.G. Da-Li, G.H. Zhang, and Q.I. A.N. Qian, 2008. Mapping of QTL underlying tolerance to alkali at germination and early seedling stages in rice. J. Acta Agronomica Sinica, 34: 1719-1727. Fakheri B., and A. Kgalegh Babaki, 2014. Mapping of genomic areas controlling the physiological and morphological traits associated with germination of bread wheat in normal condition and osmotic stress. Iranian J. Crop Sci. 45: 133-119. (In Persian, with English Abstract) Farzadmehr, J., M. Ramezani Goshk, N. Behbahani, And N. Momeni, 2011. Effect of salt stress and drought stress on seed germination and seedling growth in Salsola Arbuscula. J. Nat. Res. 64: 227-217. (In Persian, with English Abstract) Guo, P., M. Baum, S. Grando, S. Ceccarelli, G. Bai, R. Li, and J. Valkoun, 2009. Differentially expressed genes between drought-tolerant and drought-sensitive barley genotypes in response to drought stress during the reproductive stage. J Exp. Bot. 60: 3531-3544. Hu, S.P., Y. Hua, H. Zou, Y. Liu, l. Liu, C. Mei, M.S. Run, and L. Luo, 2007. Relationship between coleoptile length and drought resistance and their QTL mapping in rice. Rice Sci. 14: 13-20. Ilona, C.M., I, Izabel Marcińska, I., Edyta Skrzypek, S., Cyganek K, Juzoń1, K. and Karbarz, M. 2014. QTL mapping for germination of seeds obtained from previous wheat generation under drought. Cent. Europ. J. Bio. 9: 374-382. Kleinhofs, A., A. Kilian, M.A. Saghai Maroof, R.M. Biyashev, P. Hayes, F.Q. Chen, N. Lspitan, A. Fenwick, T.K. Blake, V. Kanazin, E. Ananiev, L. Dahleen, D. Kurdna, J. Bollinger, S.J. Knapp, B. Liu, M. Sorrells, M. Heun, J.D. Franckowiak, D. Hoffman, R. Skadsen, and B.J. Stefffenson, 1993. A molecular, isozymes, and morphological map of the barley (Hordeum vulgare L.) genome. Theor. Appl. Genet. 86: 705-712. Landjeva, S., U. Lohwasser, and A. Borner, 2010. Genetic mapping within the wheat D genome reveals QTL for germination, seed vigour and longevity, and early seedling growth. Euphytica. 171: 129-143. Léon, J., S.E. Ibrahim, A. Schubert, and K. Pillen, 2012. QTL analysis of drought tolerance for seedling root morphological traits in an advanced backcross population of spring wheat. Inter. J. Agric. Sci. 2: 619-629. Michel, B.E., and M.R. Kaufman, 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiol. 51: 914-916. Mohammed, A.S. 2011. QTL Analysis for Drought Tolerance Related to Root and Shoot Traits in Barley (Hordeum vulgare L). Ph. D. dissertation, Bonn University, Germany. Patade, V.Y., K. Maya, and A. Zakwan, 2011. Seed priming mediated germination improvement and tolerance to subsequent exposure to cold and salt stress in capsicum. Res. J. Seed Sci. 4: 125 -136. Rabiei, B., Z. Mardani, K.h. Qomi, H. Sabouri, And A. Sabouri, 2014. Effect of chromosome 1 on traits related to salinity and drought tolerance in germination and seedling stages. J. Seed and Plant Seedling 30: 1-16 (In Persian, with English Abstract) Sabouri, H., A. Biabani, A. Sabouri, M. Esmaeili, 2010. Study of QTLs related to seedling strain in photostimulated stratigraphy obtained from sorbitol in rice. J. Plant Prod. Res. 17: 136-123. (In Persian, with English Abstract) Sabouri, H. and A. Sabouri, 2008. New evidence of QTLs attributed to salinity tolerance in rice. Afric. J. Biotech. 7: 4376-4383. Sabouri, H. Sh., Mohammad Alaegh, A. Biabani, A.R. Dadras, A. Sabouri, M. Katouzi, M. Najjar Ajam, M. Pirasteh, And R. Khataminejad, 2014. Identification of gene loci for controlling germination components in Iranian Rice (Oryza sativa L.) reproductive lines under different conditions of Somatic stress. J. Crop Biotech. 8: 45-3. (In Persian, with English Abstract) Schneider, E.C., and S.C. Gupta, 1985. Corn emergence as influenced by soil temperature matric potential and aggregate size distribution. Soil Sci. Soc. Am. J. 49:415-422. Slafer, G.A., J.L. Molina Cano, R. Savin, J.L. Araus, and I. Romagosa, 2002. Barley Science: Recent Advances from Molecular Biology to Agronomy of Yield and Quality. Haworth Press, New York, USA.PP: 590. Wang, J., G. Sun, X. Ren, C. Li, L. Liu, Q. Wang, B. Du, and D. Sun, 2016. QTL underlying some agronomic trait in barley detected by SNP markers. BMC Genet. 103: 1-13. Zaare, M., and H. Jafari, 2013. Quantitative trait loci diversity for salt tolerance at the early growth stage of barley (Hordeum vulgare L.). J. Crop Breed. 3: 69-77. (In Persian, with English Abstract) | ||
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