Heidarnejad, O., Firouzeh, S., Ghaem Maghami, S., Dadashpour Davachi, N., Asghari, S. (2022). Evaluation of Accuracy of measuring concentration of estradiol 17β and progesterone of milk in pregnancy and estrus detection of Saanen goats. , 35(1), 149-156. doi: 10.22092/vj.2020.352139.1779
O. Heidarnejad; S. Firouzeh; S. S. Ghaem Maghami; N. Dadashpour Davachi; S. R Asghari. "Evaluation of Accuracy of measuring concentration of estradiol 17β and progesterone of milk in pregnancy and estrus detection of Saanen goats". , 35, 1, 2022, 149-156. doi: 10.22092/vj.2020.352139.1779
Heidarnejad, O., Firouzeh, S., Ghaem Maghami, S., Dadashpour Davachi, N., Asghari, S. (2022). 'Evaluation of Accuracy of measuring concentration of estradiol 17β and progesterone of milk in pregnancy and estrus detection of Saanen goats', , 35(1), pp. 149-156. doi: 10.22092/vj.2020.352139.1779
Heidarnejad, O., Firouzeh, S., Ghaem Maghami, S., Dadashpour Davachi, N., Asghari, S. Evaluation of Accuracy of measuring concentration of estradiol 17β and progesterone of milk in pregnancy and estrus detection of Saanen goats. , 2022; 35(1): 149-156. doi: 10.22092/vj.2020.352139.1779

Evaluation of Accuracy of measuring concentration of estradiol 17β and progesterone of milk in pregnancy and estrus detection of Saanen goats

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 17, Volume 35, Issue 1, March 2022, Pages 149-156    PDF (512.6 K)
Document Type: Full Research Paper
DOI: 10.22092/vj.2020.352139.1779
Authors
O. Heidarnejad* ; S. Firouzeh; S. S. Ghaem Maghami; N. Dadashpour Davachi; S. R Asghari
Abstract
Early pregnancy detection is a strategic way to reduce farm costs. Nutritional and health costs resulting from the maintenance of non-pregnant animals will not be imposed on the farmer, by timely detection of pregnancy. The aim of this study was to evaluate the accuracy of early detection of estrus and pregnancy in Sannan goats by measuring of estradiol-17β and progesterone concentrations in milk. In this experiment, 22 Sannan goats with 2-3 years old, with an average calving of 1.75 and an average weight of 47.5 ± 3.45Kg were selected and kept under equal conditions. First, estrus was synchronized in goats with vaginal sponge and PMSG and prostaglandin hormones (sponge for 12 days and injection of hormones on the final day). Milk samples were taken on days 0 and 1 of the estrous cycle (for estradiol test and estrus diagnosis) and on days 19, 21, 23 and 25 after mating (for progesterone measurement and pregnancy diagnosis). The average concentration of estradiol in milk was 15.01 pg/ml on day 0 and 2.76 pg/ml on day 1 of estrus. Based on sources, animals whose milk progesterone was more than 7.5 ng/ml were assumed to be pregnant, which was evaluated 50 days later by ultrasonography. Results show that Milk progesterone can be a way to diagnose goat pregnancy from the 19th day after mating with 86% accuracy. In summary, measuring milk estradiol and progesterone can be a way to timely estrus detection and to early diagnosis of pregnancy.
 
Keywords
Estrus detection; 17 β Estradiol; Progesterone; Pregnancy detection; Saanen goat; milk
References

1.Blaszczyk, B.,J. Udala, D. Gaczarzewicz. 2004. Changes in estradiol, progesterone, melatonin, prolactin and thyroxine concentractions in blood plasma of goats following induced estrus in and outside the natural breeding season. Small Rum. Res. 51: 209-19
2.Buckrell, B.C. 1988. Applications of ultrasonography in reproduction sheep and goats. Theriogenology 29:71–84.
3.DeCastro, T., E. Rubianes, A. Menchaca, A.Rivero. 1999. Ovarian dynamics, serum estradiol and progesterone concentrations during the interovulatory interval in goats. Theriogenology 52:399–411.
4.Dionysius, D.A. 1991. Pregnancy diagnosis in dairy goats and cows using progesterone assay kits. Australian Veterinary Journal 68(1): 14-6. doi: 10.1111/j.1751-0813.1991.tb09832.x 
5.Domènech, A., S. Pich, A. Arís, C. Plasencia, A. Bach. 2011. Heat identificationby 17ß-estradiol and progesterone quantification in individual raw milk samples byenzyme immunoassay. Electron J Biotechn 14: 1-5.
6.England, I.V., E. Ropstad, Ø. Andesen, L. O. Eik. 1997. Pregnancy diagnosis in dairy goats using progesterone assays kits and oestrus observation. Anim. Reprod. Sci. 47: 237-43. 
7.Fleming, S.A., S.D. Van Camp, H.M. Chapin. 1990. Serum progesterone determination as an aid for pregnancy diagnosis in goats bred out of season. Can. Vet. J. 31:104–7.
8.Gonza´lez, F., J. Sulon, P. Calero, , M.Batista, A.Gracia, J. F. Beckers. 2001. Pregnancy-associated glycoproteins (PAG) detection in milk samples for pregnancy diagnosis in dairy goats. Theriogenology; 56:671–6.
9.Gorecki, M.T., J. Wojtowski, P. Kaczmarek. 2004. Concentrations of progesterone and 17b-estradiol in blood and milk and those of natural inhibitors in milk of goats in various physiological stages. Arch Tierz (special issue) 47:90–96.
10.Hafez, E. S. E., and B. Hafez. 2000.  Reproduction in Farm   Animals, 7th Ed.  Lippincott Williams and Wilkins, Philadelphia. 
11.Heap, R. B., R.J. Holdsworth, J.E. Gadsby, J.A.Laing, D. E. Walters. 1971. Pregnancy diagnosis in the cow from milk progesterone concentraction. Br. Vet. J. 132: 445-464
12.Ishwar, A.K. 1995. Pregnancy diagnosis in sheep and goats: a review. Small Rumin Res 17:37–44.
13.Mccoy, M. A., C. S. Mayne, S. D. Lennox, M. Verner, D. R. Mackey, A. R. G. Wyle, , F. J.  Gordaon. 2002. Fertility and milk progesterone profiles in dairy herds in Northern Ireland. Res. Vet. Sci. 72 Suppl 1, 9-9. DOI: 10.1016/S0034-5288(02)90027-5 
14.Mosaferi, S. Y., V. Nikraftar Mashhadi, L. Ghaffari. 2007. Evaluation of the accuracy of pregnancy diagnosis in cattle by measuring milk progesterone by ELISA method. Iranian Veterinary Journal 3(1): 71-77.
15.Montigny.,G.D., P. Millerioux, , N. Jeanguyot, P. Humblot, , M. Thibier. 1982. Milk fat progesterone concentration in goat and early pregnancy diagnosis. Theriogenology 17(4): 423-431.
16.Pennington, J. A., S. L. Spahr, , J. R. Lodge. 1982. Influences on progesterone concentration in bovine milk. J. Dairy Sci. 64:259-267.  
17.Pennington, J. A., S. L. Spahr, J. R. Lodge, D. Considine. 1977. Progesterone content of goat'smilk and its application for pregnancy diagnosis. J. Dairy Sci. 60. (Suppl. 1):82. (Abstr.)
18.Roelofs, J. B., F. J. Van Eerdenburg, W. Hazeleger, N.M.  Soede, B. Kemp. 2006. Relationship between progesterone concentrations in milk and blood and time of ovulation in dairy cattle. Short communication. Anim Reprod Sci 91: 337-343.
19.Thibier, M., D. Pothelet, N. Jeanguyot, G. DeMontigny. 1981. Estrus behavior, progesterone in peripheral plasma and milk in dairy goats at onset of breeding season. J. Dairy Sci. 64:513-521.
20.Worsfold A. I.,  J. M. Booth, A. C. Huddart,  C.J. Stanley. 1987. The evaluation of a new rapid milk progesterone test as an aid to improving dairy herd fertility. Br Vet J 143:83-87.

 

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