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Induced resistance to fusarium tomato wilting disease throught increase of antioxidant enzymes and pathogenesis –related genes using MethylJasmonat | ||
| آفات و بیماری های گیاهی | ||
| Volume 88, Issue 2 - Serial Number 111, March 2021, Pages 275-284 PDF (638.19 K) | ||
| Document Type: Plant Pathology | ||
| DOI: 10.22092/jaep.2020.342199.1332 | ||
| Authors | ||
| masoud Golesrtani1; Mohammad Rezaa Sarafraz ardakani2; benyamin Kazempour3; marziyeh Tahei4 | ||
| 1Peyamnoor University of Yazd | ||
| 2Department of Biology, Faculty of Science, Yazd University | ||
| 33- MSc student of Biotechnology, Department of Biotechnology, Faculty of Natural Resources of Desert , Yazd niversity | ||
| 41- Department of Biology, Faculty of Science, Yazd University | ||
| Abstract | ||
| In this research, possibility of induced systemic resistance in tow tomato cultivars Termeh and Capitan was assayed by exogenous application of methyl jasmonat (MeJA). Tomato plants were treated with methyl jasmonat at concentration of 0, 0.1 and 0.2 mM by spraying at 2-4 leaf stage and then were inoculated withpathogen agent at 6- leaf stages. Gene expression level of npr1, pds and eds1 genes and catalase, peroxidase, polyphenol oxidase activity and total phenol continent was investigated using qRT-PCR and spectrophotometery methods, repectively. The results of this research showed that enzyme activity was variable in tested cultivars and interval times after inoculation. The highest enzyme activity was observed in Termeh cultivar under disease stress. The highest and lowest expression level was occured in npr1 and pds genes, respectively. These results indicated that methyl jasmonat is able to iduces resistance in tomato against wilting disease throught change on bio-chemical and molecular activities. | ||
| Keywords | ||
| Acquired resistance; gene expression; methyl jasmonat; transcription factors | ||
| References | ||
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