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Investigation of various genome editing methods using targeted nuclease enzymes | ||
| علوم و فنون دامپروری | ||
| Volume 10, Issue 40, December 2021, Pages 47-60 PDF (1.76 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/aasrj.2021.354589.1235 | ||
| Authors | ||
| Hamed Asadollahi* 1; Hossein Emrani2 | ||
| 1Department of Animal Science, Faculty of Agriculture, Isfahan University of Technology, Isfahan, Iran | ||
| 2Animal Science Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), 31585 Karaj, Iran | ||
| Abstract | ||
| In recent decades, genetic engineering has relied on techniques that took years, as well as the construction of complex DNA constructs such as selective markers that were used for only a few specific species. With the advent of targeted nuclease enzymes, including ZFN, TALEN, and CRISPR-Cas9 technologies, the genome of different species are easily editable. Targeted nuclease enzymes are used in inactivation of specific genes in livestock to improve animal production traits and increase productivity, animal models to study various human diseases, reduce the transmission of common human and animal diseases, Inactivation of genes involved in various infections, resistance to pathogens, and finally modification and treatment of complex genetic disorders. This developments has provided countless opportunities that not only contribute to our understanding of gene functions and disease mechanisms, but also to the identification of various pathogenic mutations, the improvement of agricultural and livestock products, and the improvement of our environment. | ||
| Keywords | ||
| Genome editing; Targeted nuclease enzymes; ZFN; TALEN; CRISPR-Cas9 | ||
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