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Molecular epidemiology of American foulbrood disease in bee colonies in eight provinces of Iran | ||
| تحقیقات دامپزشکی و فرآوردههای بیولوژیک | ||
| Article 15, Volume 36, Issue 1, March 2023, Pages 113-119 PDF (587.79 K) | ||
| Document Type: Full Research Paper | ||
| DOI: 10.22092/vj.2022.357904.1952 | ||
| Authors | ||
| mojtaba moharrami* 1; hossein modirrousta2; maryam torkaman3; Hamid Reza Jalali4; Kamyar Ahmadi5; Mohammad Hossien Fallah Mehrabadi3 | ||
| 1Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension, Karaj. Iran | ||
| 2Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension. Karaj.Iran | ||
| 3Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension. Karaj. Iran | ||
| 4Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension. Karaj . Iran | ||
| 5Iran Veterinary Organization | ||
| Abstract | ||
| American foulbrood disease is the most serious and deadly bacterial disease of bee infants and is caused by the gram-positive bacterium that produces Paenibacillus larvae spores. In this regard, accurate and timely diagnosis of this disease is very important. In the present study, the molecular epidemiology of this disease was studied regionally in eight provinces of the country. Frothy eight samples of bees, honey and debris were collected. After preparation, the samples were tested by nested PCR method and the PCR product was electrophoresed on 1% agarose gel. The results show out of 48 samples of bees examined, number of 14 were positive (29.16%), out of 48 samples of honey were examined, number of 16 were positive (33.33%) and out of 48 samples of debries, number of 5 were positive (10.41%). The results of this study show that for epidemiological and monitoring studies of Paenibacillus larvae infection in bee colonies, collecting honey samples is a more reliable method than other samples. Today in the world, this method is used for continuous monitoring of this disease and according to the results, the strategy of control, prevention and treatment are formulated and implemented. Also, diagnosis of the disease using honey samples and in the next priority of adult bees, has a higher prognostic value compared to the identification of bacteria in the samples of wax, pollen and debris. | ||
| Keywords | ||
| American foulbrood; Paenibacillus larvae; Nested PCR | ||
| References | ||
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