Related Links
Virological and werological wurvey on bluetongue disease in sheep in some parts of Iran | ||
| تحقیقات دامپزشکی و فرآورده‌های بیولوژیک | ||
| Editorial, Volume 26, Issue 2, June 2013, Pages 36-43 PDF (1.11 M) | ||
| DOI: 10.22092/vj.2013.101031 | ||
| Authors | ||
| R Fallahi* 1; M Yaghini2; R Kargar Moakhar3; K Khedmati4 | ||
| 1Assistant Professor of Razi Vaccine and Serum Research Institute, Karaj | ||
| 2Expert of Razi Vaccine and Serum Research Institute | ||
| 3Professor of Razi Vaccine and Serum Research Institute | ||
| 4Instructor Razi Vaccine and Serum Research Institute | ||
| Abstract | ||
| Bluetongue is an acute and subacute viral diseases of domestic and wild ruminants.This research was conducted to assess the disease status using serological diagnostic methods and virus isolation were performed. During the period 2003-2006, from sheep farms of 9 provinces including Markazi, Ardabil, Yazd, Qom, East Azarbaijan, West Azarbaijan, Qazvin, Isfahan and Golestan, Four handred thirty two samples collected in suspected sheep to disease. In first stage, by ELISA method, the antibody against bluetongue virus were found in two hundred eighty seven samples. For virus isolation, from samples of buffy coats, sixty five samples inoculated to embryonated SPF eggs and after virus propagation, re-inoculated into Vero and BHK cell cultures. In twenty two inoculated samples, the cell lesions was observed. With serum neutralization test confirmed. After titration the virus, preparation for observation under electron microscopy done, and, viral particles were found in 70x72nm. After injection the purified virus and whole the primary samples, sensitive sheep,the clinical signs seen. After obtaining blood samples from sheep infected, the all of the steps was again the results confirm there is bluetongue virus. | ||
| References | ||
|
1- بکایی، س.کارگر‌موخر، ر. موسوی، م . شریفی، ل. رامین، ع. ارس خانی، ع (1386) بررسی سرولوژیک بیماری زبان آبی (Bluetongue) در گله های گوسفند آذربایجان غربی، مجله دامپزشکی ایران، دوره سوم شماره ٣، صفحه 83-81.## 2- خان بابائی، ه. فکور، ش. خضری، م. محمدیان، ب. رخزاد، ب. (1390) بررسی سرولوژیک بیماری زبان آبی در گوسفندان شهرستان سنندج به روش الیزا، مجله دامپزشکی دانشگاه آزاد اسلامی، دوره 5، شماره 2. ## 3- Afshar, A., Thomas, F.C., Wright, P.F., (1989) Compression of competitive Elisa, Indirect Elisa and standard AGID tests for detecting blue tongue virus antibodies in cattle and sheep, Veterinary Record, 124: 136-141## 4- Alexander, R. A., HAG, D.A., (1951) The use of egg attenuated blue tongue in the production of polyvalent vaccine for sheep, A propagation of the virus in sheep, Journal of Veterinary Research, 25: 3-15## 5- Billinis, C., Koumbati, M., Spyrou, V., Nomikou, K., Mangana, O., Panagiotidis, C. A., Papadopoulos, O., (2001) Bluetongue virus diagnosis of clinical cases by a duplex reverse transcription-PCR: a comparison with conventional methods, Journal of Virological Methods, 98: 77-89## 6- Cheiron, A., (1997) Isolation and characterization of blue tongue Virus, Veterinary Research,53: 17-26## 7- Ciavijo, A., Heckert, R.A., Dulac, G.C., Afshar, A., (2000) Isolation and identification of blue tongue virus, Journal of Virological Methods, 87: 13-23## 8- Breard, E., Hamblin, C., Hammoumi, S., Sailleau, C., Dauphin, G. and Zientara, S. (2004) The epidemiology and diagnosis of blue tongue with particular reference to Corsica, Research in 9- Darpel, K.E., Batten, C. A., Veronesi, E., Shaw, A. E., Anthony, S., Bachanek-Bankowska, K., Kgosana, L., bin-Tarif, A., Carpenter, S., Müller-Doblies, U., Takamatsu, H., Mellor, P. S., Mertens, P., Oura, C., (2007) Clinical signs and pathology shown by British sheep and cattle infected with bluetongue virus serotype 8 derived from the 2006 outbreak in northern Europe. Veterinary Record 161(8): 253-261## 10- David, E., Swyne, E., Chairman, J., (1998) Labolatory manual for the isolation & characterization of avian patogens, Forth edition by the American accesories of avian pathologists.## 11- Davies, F.G., Mungai, J.N., Pini, A., (1992) A new bluetongue virus serotype isolated in Kenya, Veterinary Microbiology, 31(1): 25-32## 12- Erasmus, B.J., (1975) Blue tongue in sheep and goats, Australian Veterinary Journal, 51: 165-170 ## 13- Ertürk, A., Tatar, N., Kabakli, O., Incoglu, S.,Cizmeci, G.S. and Barut, F. M. (2004) The current situation of bluetongue in Turkey, Veteterinaria Italiana, 40(3):137-140.## 14- FAO (2002) Food and Agricaltural of the United Nations ## 15- Fenner F.J. (1992) Veterinary Virology, The John Curtin School of Medical Research## 16-Gard, P.S., Kirkland, P. D.,(1993) Blue tongue virology and serology in: Australian standard diagnostic techniques for animal diseases, Corner L.A.S Barguest, T.J. ends. CSIRO information servies## 17- Hawkeys, R.A., Kirkland, P.D., Sanders, D.A., Zhang, F.L.Z., (2000) Laboratory and field studies of an antigen capture Elisa for Blue Tongue virus, Journal of Virological Methods, 85: 137-179 ## 18- Jochin, M. M., Chow, T. I., (1969) Immunodiffusion of bluetongue virus, American Journal of Veterinary Research, 30 (1) 33-41## 19- Koumbati, M., Mangana, O., Nomikou, K., Mellor, P.S., Papadopoulos, O., (1999) Duration of bluetongue viraemia and serological responses in experimentally infected European breeds of sheep and goats, Veterinary Microbiology, 64 (4): 277-285## 20- Lee, V.H., Causey, O.R., Dorothy, L., Moore, L., (1974) Blue tongue and related viruses in Ibadan, Nigeria: Isolation and preliminary identification of viruses, American Journal of Veterinary Research, 35 (8): 1105- 1108## 21- OIE (Office International Des Epizooties), (2004) Isolation and characterization of blue tongue, Third International Symposium on Blue tongue, 195-210## 22- OIE (Office International Des Epizooties), (2005) Terrestrial Manual Blue tongue, Chapter 2.1.9.## 23- Reddington, J.J., Reddington, G.M., MacLachlan, N.J., (1991) A competitive ELISA for detection of antibodies to the group antigen of bluetongue virus, Journal of Veterinary Diagnosis Investigation, 3: 144-147.## 24- Sreenivasulu, D., Rao, M., Gard, G.P., (1999) Isolation of bluetongue virus serotype 2 from native sheep in India, Veterinary Record, 144 (16): 452-453## 24-Wittman, E., Baylis, M., (2000) Climate change: effects on Culicoides-transmitted viruses and implications for the UK, The Veterinary Journal, 160: 107-117 ## | ||
|
Statistics Article View: 1,401 PDF Download: 1,301 |
||
Journal management system. designed by sinaweb