Mahdizadehnaraghi, R., Heydari, A., Zamanizadeh, H., Rezaee, S., Nikan, J. (2015). Biological control of Sclerotium cepivorum, the causal agent of Allium (garlic) white rot disease, using Trichoderma and Talaromyces fungal antagonists in the laboratory and green house conditions. , 2(2), 13-24. doi: 10.22092/bcpp.2015.101223
Razak Mahdizadehnaraghi; Asghar Heydari; Hamidreza Zamanizadeh; Saeed Rezaee; Jafar Nikan. "Biological control of Sclerotium cepivorum, the causal agent of Allium (garlic) white rot disease, using Trichoderma and Talaromyces fungal antagonists in the laboratory and green house conditions". , 2, 2, 2015, 13-24. doi: 10.22092/bcpp.2015.101223
Mahdizadehnaraghi, R., Heydari, A., Zamanizadeh, H., Rezaee, S., Nikan, J. (2015). 'Biological control of Sclerotium cepivorum, the causal agent of Allium (garlic) white rot disease, using Trichoderma and Talaromyces fungal antagonists in the laboratory and green house conditions', , 2(2), pp. 13-24. doi: 10.22092/bcpp.2015.101223
Mahdizadehnaraghi, R., Heydari, A., Zamanizadeh, H., Rezaee, S., Nikan, J. Biological control of Sclerotium cepivorum, the causal agent of Allium (garlic) white rot disease, using Trichoderma and Talaromyces fungal antagonists in the laboratory and green house conditions. , 2015; 2(2): 13-24. doi: 10.22092/bcpp.2015.101223

Biological control of Sclerotium cepivorum, the causal agent of Allium (garlic) white rot disease, using Trichoderma and Talaromyces fungal antagonists in the laboratory and green house conditions

مهار زیستی در گیاه‌پزشکی
Article 2, Volume 2, Issue 2, February 2015, Pages 13-24    PDF (204.89 K)
Document Type: Research Paper
DOI: 10.22092/bcpp.2015.101223
Authors
Razak Mahdizadehnaraghi* ; Asghar Heydari; Hamidreza Zamanizadeh; Saeed Rezaee; Jafar Nikan
Abstract
White rot caused by Sclerotium cepivorum (Berk).is one of the most widespread and damaging fungal diseases of garlic This diseas causes significant losses to garlic crops in Iran including Hamadan province evey year. At the present, the most common control control method of the disease is the use of chemical fungicides. This control measure is costly and contaminates the agricultural environment. Moreover, since the pathogen is soil-borne, this control method is not quite effective in controlling the disease. In this study, the effects of two antagonistic fungi including Trichoderma and Talaromyces as bio-control agents in the control of the causal fungal pathjogen were investigated under laboratory and greenhouse conditions. For this purpose, ten isolates of the antagonistic fungi, four isolates of T. harzianum (Th), two isolates of T. asperellum (Ta) and four isolates of T. flavus (Tf) were selected. The effects of antagonistic agents on the growth of the pathogen were evaluated in in vitro using dual culture method. Moreover, the impact of the volatile and non-volatile metabolites of the antagonistic agents on growth of the pathogen was also investigated. The results of the laboratory experiments showed that six isolates (two isolate from each species) significantly reduced the pathogen growth. Several new bioformulations were then developed and prepared using rice bran (RB) as an organic carrier and the most effective antagonistic isolates. The effectiveness of the developed bioformulations in controlling the white rot disease of garlic was studied under green-house conditions. The experiment was conducted as completely randomized design consisted of nine treatments (six bioformulations, one fungicide and two controls) each with four replications. The results of the greenhouse experiments indicated that regarding disease incidence and severity, there were significant differences among the treatments (P<0.05). The highest infection rate (53.82%) was observed in the positive control (treatment without biological agent or fungicide). The lowest infection rates were belonged tohealthy control (9.24%), fungicide (11.23%), RBTh1 (11.33%), RBTa2 (11.37%) and RBTh2(11.42%) respectively which were placed in the same statistical group.These overall results indicate that the biological control of Allium white rot disease using antagonistic fungi is possible as an effective alternative method.
Keywords
Allium White Rot Disease; Biological control؛ Formulation؛ Garlic; Rice bran
References

Abdolahi, M. Ommati, F. & Zaker, M. 2013. Efficacy of some native Trichoderma isolates in biological control of Pythium aphanidermatum, the causal agent of sugar beet root rot under green house condition. Biocontrol in Plant Protection, 1 (1) 41-52.

Anonymous. 2011. Agricultural Information Bank. Management of Plan and program Agriculture Organization Hamedan province. http://www.iana.ir /9895-1.html.

Bakonyi, j. Vajna, L. Szeredi, A. Tímár, E. Kovács, G.M. Csősz, M. & Varga, A. 2011. First Report of Sclerotium cepivorum causing white rot of garlic in Hungary. New Disease Reports. Phytopathology, 87:112-117.

Barnett, H. L. & Hunter, B. 1998. Illustracted genera of Imperfect Fungi. American Phytopathological Society, 218 pp.

Beheshti, B. Sharifi-Sirchi,G.R. Mansouri, M. Hosseinipour, A. & Schlaich, N.L. 2011. Resistanceto citrus canker in key mexican lime induced by β-aminobutyric acid and green tea. American journal of Agricultural and biological Sciences, 6: 242-248.

Berk, M. 1841. Sclerotium cepivorum, Annals and Magazine of Natural History. Series 1:6-359.

Clarkson, P. Payne, T. Nmead, A. & Whipps, J. 2002. Selection of fungal biological control agents of Sclerotium cepivorum for control of white rot by sclerotial degradation in a UK soil. Plant Pathology, 51: 735-745.

Davis, R.M. Aegerter, B. Laemmlen, F. F. & Voss, R.E. 2007. Onion and garlic white rot. Reviewed 1/07 University of California agriculture and natural resources.

Entwistle, A., R. 1990. Allium white rot and its control. Soil Use and Management. 6: 201-209.

Francisco, D.H. Angelica, M.P. Gabriel, M. Melchor, C.S. Raul, R. Cristobal, N. & Francisco, C.R, 2011. In vitro antagonist action of Trichoderma strains against Sclerotium cepivorum and Sclerotinia sclerotirum. American journal of Agricultural and biological Sciences, 6(3): 410-417.

Hajimashaalahbazaz, S. Razavi, M. & Ghasemi, A. 2013. Evaluation of Pseudomonas fluorescens for biological control of wheat crown and root rot disease (Fusarium culmorum). Iranian Journal of Biocontroln in Plant Protection, 1(2): 2-16.

Heydari, A. & Naraghi, L. 2014. Application of antagonistic bacteria for the promotion of cotton seedlings growth characteristics. International Journal of Agriculture and Crop Sciences, 7(13): 1267-1273.

Jorjani, M. Heydari, A. Zamanizadeh,H.R. Rezaee, S. & Naraghi, L. 2011. Development of Pseudomonas fluorescens and Bacillus coagulans based bioformulations using organic and inorganic carriers and evaluation of their influence on growth parameters of sugar beet. Journal of Biopesticides, 4 (2): 180-185.

Kakvan, N. Heydari, A. Zamanizadeh, H.R. Rezaee, S. & Naraghi, L. 2013. Development of new bioformulations using Trichoderma and Talaromyces fungal antagonists for biological control of sugar beet damping-off disease. Crop Protection, 53: 80-84.

Keller, E.R.J. Senula, A. & Dreiling, M, 2005. Gene banking of vegetative propagated medicinal plants – two cases: Allium and Mentha. Journal of ActaHortic, 676: 103 – 109.

Leta, A. & Selvaraj, Th. 2013. Evalution of Arbuscular mycorrizal fungi and Trichoderma species for the control of onion white rot (Sclerotium cepivorum Berk). Plant Pathology Microbiology, 4(159):1-6.

Mahdizadehnaraghi, R. Zafari, D. Zamanizadeh, H.R. & Arjmandian, A. 2007. Identification of the fungal disease agents on garlic in Hamedan province. Agricultural Research (Water, Soil, Plant in agriculture), 7(3): 11-29.

McLean, kirstin, L. 2001. Biological control of onion white rot using Trichoderma harzianum. Thesis submitted in fulfilment of the requirements for the Degree of Doctor of Philosophy at Lincoln University.http://hdi.handle.net/10182/1494.

Metcalf, D.A. Dennis, J.J.C. & Wilson, C.R. 2004. Effect of inoculum density of Sclerotium cepivorum on the ability of Trichoderma koningii to suppress white rot of onion. Plant Disease, 88 (3): 287-291.

Naeimi, S. & Zare, R. 2013. Evaluation of indigenous Trichoderma spp. isolates in biological control of Botrytis cinerea the causal agent of strawberry gray mold disease. Iranian Journal of Biocontrol in Plant Protection, 1 (2): 52-74.

Naraghi, L. Heydari, A. & Rezaee, S. 2005. Study of Antagonistic Mechanisms and  action different isolates Talaromyces Flavus and Determination of genetic diversity. The thesis submitted for the degree of PhD. in field of plant pathology, Islamic Azad University Science and Research Branch, Tehran. Iran.

Naraghi L., Heydari A., Rezaee S., & Razavi M. 2013. Study on some antagonistic mechanisms of Talaromyces flavus against Verticillium dahliae and Verticillium albo-atrum, the causal agents of wilt disease in several important crops. Biocontrol in Plant Protection, 28 (1): 13-28.

Ricardo, J. Zavaleta, E. & Aguilera, G. 2000. Variability of four Mexican isolates of Sclerotium cepivorum. Revista Mexicana Phytopathologia, 103-110.

Samavat, S. Heydari, A., Zamanizadeh, H. R. Rezaee, S. & Alizadehaliabadi, A. 2014. Comparison between Pseudomonas aureofaciens (chlororaphis) and P. fluorescens in biological control of cotton seedling damping-off disease. Iranian Journal of Plant Protection Science, 54 (2): 115-121.

Saremi, H. Ammarellou, A. & Saremi, H. 2010. Garlic white rot caused by Sclerotium cepivorum and its managing by soil solarization in Zanjan province, northwest Iran. Journal of food, Agriculture & Environment, 8(3, 4): 411-414.

Sikora, R.A. & Hoffman-Hergarten, S. 1993. Biologycal control of plant parasitic nematodes with plant health promoting rhizobacteria, in pest management: Biologically based technologies, proceeding of Beltsville symposium XVIII, edited by Iumsden Redland Vaughn, J.L. American Chemical Society. Washington, 166-172.

Statistics
Article View: 5,275
PDF Download: 2,125


counter
Journal management system. designed by sinaweb