Banani,, M., Karimi,, G. (2015). Propagation of Cryptosporidium baileyi in the chicken embryonated SPF eggs. , 28(2), 44-50. doi: 10.22092/vj.2015.101270
M. Banani,; Gh.R. Karimi,. "Propagation of Cryptosporidium baileyi in the chicken embryonated SPF eggs". , 28, 2, 2015, 44-50. doi: 10.22092/vj.2015.101270
Banani,, M., Karimi,, G. (2015). 'Propagation of Cryptosporidium baileyi in the chicken embryonated SPF eggs', , 28(2), pp. 44-50. doi: 10.22092/vj.2015.101270
Banani,, M., Karimi,, G. Propagation of Cryptosporidium baileyi in the chicken embryonated SPF eggs. , 2015; 28(2): 44-50. doi: 10.22092/vj.2015.101270

Propagation of Cryptosporidium baileyi in the chicken embryonated SPF eggs

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 7, Volume 28, Issue 2, July 2015, Pages 44-50    PDF (597.26 K)
Document Type: Full Research Paper
DOI: 10.22092/vj.2015.101270
Authors
M. Banani,* 1; Gh.R. Karimi,2
1Associate Professor of Razi Vaccine & Serum Research Institute, Iran. Karimi,
2Assistant Professor of Razi Vaccine & Serum Research Institute, Iran.
Abstract
Cryptosporidium baileyi is one of the pathogen protozoa which is specific for poultry. C. baileyi is able to infect, grow and reproduce in epithelial cells of respiratory, gastrointestinal and urinary tracts of chickens. This parasite has been isolated from Iran poultry industry. The aim of this study was to propagate the parasite in the chicken embryonating SPF eggs. Oocysts were maintained by passage through chicks. Faeces containing excreted oocysts were mixed with 2.5% potassium dichromate (w/v) and stored at 4°C. Oocysts of C. baileyi were purified and cleaned by modification of Sheater’s sugar flotation method and using 1% sodium hypochlorite solution.  The oocysts were washed with PBS and appropriate antibiotics were added. Ten day- old embryonating SPF eggs were inoculated via allantoic cavity with 1x 105 oocysts or equivalent oocysts exposed by excystation fluid. The eggs inoculated by samples were incubated 7 days at 37 ºC and candled once a day. The results showed that both inoculated samples were able to grow and propagate in chorio allantoic membrane and release the oocysts in allantoic fluid. Inoculation of 1x105 oocysts caused releasing of 1x106 oocysts in the allantoic fluid of one egg. Upon the results the propagation of C. baileyi in the chicken embryonating SPF eggs can be used in research works. This method can be used for limited propagation of the parasite using the intact oocysts as inoculated samples in embryonating eggs of chickens or even other birds.. 
Keywords
Propagation; Oocyst; Cryptosporidium baileyi; chicken embryonating SPF egg
References

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