alipanah, M., Irankhah, A. (2015). Identification BF2*21 and BF2*13 alleles of Major Histocompatibility Complex in two Native Chickens, Khazak and Dashtiari. , 28(106), 259-264. doi: 10.22092/asj.2015.101365
masoud alipanah; Adeleh Irankhah. "Identification BF2*21 and BF2*13 alleles of Major Histocompatibility Complex in two Native Chickens, Khazak and Dashtiari". , 28, 106, 2015, 259-264. doi: 10.22092/asj.2015.101365
alipanah, M., Irankhah, A. (2015). 'Identification BF2*21 and BF2*13 alleles of Major Histocompatibility Complex in two Native Chickens, Khazak and Dashtiari', , 28(106), pp. 259-264. doi: 10.22092/asj.2015.101365
alipanah, M., Irankhah, A. Identification BF2*21 and BF2*13 alleles of Major Histocompatibility Complex in two Native Chickens, Khazak and Dashtiari. , 2015; 28(106): 259-264. doi: 10.22092/asj.2015.101365

Identification BF2*21 and BF2*13 alleles of Major Histocompatibility Complex in two Native Chickens, Khazak and Dashtiari

علوم دامی
Article 23, Volume 28, Issue 106, June 2015, Pages 259-264    PDF (591.66 K)
Document Type: Research Paper
DOI: 10.22092/asj.2015.101365
Authors
masoud alipanah* ; Adeleh Irankhah
Abstract
The aim of this research was to study two alleles BF2*13, BF2*21 of BF2 gene in Class I MHC in two population at Sistan and Baluchestan Province. MHC is a gene cluster that play regulation role in immune system. Many experiments showed association between the gene cluster and resistance and sensitiveness against different diseases and, also productivity traits. BF2 gene is among associated loci. In the research, blood samples were collected randomly from 72 native chickens (37 Khazak and 35 Dashtiari chickens). After DNA extraction from blood samples with standard kit, the quantitative and quality of DNA were determined by gel agarose 1%. Next stage would be designing appropriate primers for BF2*13 and BF2*21 alleles. Using PCR this alleles were amplified and PCR product observed by gel electrophoresis which were 52bp and 197 bp, respectively. Frequency of samples contain BF2*13 allele in two populations Khazak and Dashtiari observed 75.6 and 22.8%, respectively. Also frequencies of samples contain BF2*21 allele in two populations Khazak and Dashtiari were 45.9 and 34.2%, respectively. In other hand, in Khazak population that frequency samples contain BF2*13 alleles is high, allele frequency samples contain BF2*21 allele that associated with resistance to Marek disease and in flu and IBD and some productive traits, is relative property, then animal breeders could be use allele frequency of these alleles in breeding programming.
Keywords
Major Histocompatibility Complex; BF2*13; BF2*21; Native chickens
References

Boonyanuwat, K., Thummabutra, S., Sookmanee, N., Vatchavalkhu, V. and Siripholvat, V. 2006. Influences of major histocompatibiliy complex class I heliotypes on avian influenza virus disease traits in Thai indigenous chicken. Animal Science, 77: 285-289. 

Briles, W. E., McGibbon, W. H. and Irwin, M. R. 1950. On multiple alleles affecting cellular antigens in the chicken. Genetics, 35: 633-652. 

Bumstead, N. 1998. Genetic resistance to avian viruses. Rev. ci. Tech, 17: 249–255.

Dietert, R. R., Taylor, J. r. and Dietert, M. F. 1991. Biological function of the chicken major histocompatibility complex. Crit. Rev. Poult. Biol, 3:111–129.

Dunnington, E. A., Briles, W. E.,  Briles, R. E. and Siegel, P. B. 1996. Immunoresponsiveness in chickens: association of antibody production and the B system of the major histocompatibility complex. Poult. Sic., 75: 1156-1160.

Ewald, S. J., Avendano, S., Mcleod, S., Lamont, S. J. and Dekkers, J. C. M. 2007.

Associations of BF2 alleles with antibody  titres and production  traits in commercial pure line broiler chickens. Animal. Genetics, 38: 174-176.         

Gavora, J. S., Simonsen, M., Spencer, J. F., Fairful, R.W. and Gowe, R. S. 1986. Changes in the frequency of major histocompatibility haplotypes in chickens under selection for both high egg production and resistance to Marek’s disease. Z. Tierzucht Zuchtungsbiol, 103:218–226.

Juul-Madsen, HR., Dalagard, T. S., Salomonsen, J. Heller D. E. 2004. Genetic resistance with focus on Major Histocompatibility Complex.        

Livant E.J., Brigati J.R. and Ewald S. J. 2004. Diversity and locus  specificity of chicken MHC class I sequences. Animal Genetics, 35: 18–27.

Livant, E. J. and Ewald, S. J. 2005. High- resolution typing for chicken BF2 (MHC Class I) alleles by automated sequencing. Animal Genetics, 36: 432- 434.

Miller, M. M., et al. 2004. Nomenclature for the chicken major histocompatibility (B and Y) complex. Immunogenetics, 56: 261–79.

Nishibori, M., Hayashi T., Tsudzuki M., Yamamoto, Y. and Yasue, H. 2001. Complete sequence of the Japanese quail (Coturnix japonica) mitochondrial genome and its genetic relationship with related species. Animal Genetics, 32: 180-185. 

Qian Yan, R., Shengli, X., Yao Yang, T. and Xia, C. 2005. Characterization of BF2 and B2m in three Chinese Chicken lines. Immunology and Immunopathology, 108: 417-425.

Rammenssee, H.G., Falk, K. and Rotzsche, O. 1993. MHC molecules as peptides receptors. Curr.Opin.Immunol., 5: 35-44.

Sander, J. F. 1993. The major histocompatibility complex and its role in poultry production. World’s Poult. Sci. J., 49: 132–138. 

Singh, V. K., Mangalam, A. K., Dwivedi, S., Naik, S. 1998. Primer premier: program for  design of degenerate primers from a protein sequence. Biotechniques, 24:318-319.

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