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Phytochemical investigation of Withania coagulans (Stocks) Dunal in natural habits of Sistan and Baluchestan province of Iran | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| Article 4, Volume 31, Issue 3 - Serial Number 71, August 2015, Pages 406-417 PDF (1.53 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/ijmapr.2015.101792 | ||
| Authors | ||
| M. Valizadeh* 1; A. Bagheri1; J. Valizadeh2; M.H. Mirjalili3; N. Moshtaghi1 | ||
| 1Department of Biotechnology and Plant Breeding, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| 2Department of Biology, College of Science, University of Sistan and Baluchistan, Zahedan, Iran | ||
| 3Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran | ||
| Abstract | ||
| Withania coagulans (Stocks) Dunal (fam. Solanaceae) is a multipurpose medicinal plant, mainly distributed in southeastern parts of Sistan and Baluchestan province. The important medicinal properties of W. coagulans are attributed to the presence of steroidal lactones called withanolides. Among withanoloids, withaferin A is an important phytoconstituent showing antitumor, antiangiogenesis and apoptosis induction properties. This study was aimed to investigate the variability of phytochemical composition of Withania coagulans roots. Twenty accessions of W. coagulans roots, collected from different natural habits of Sistan and Baluchestan province, were used in the present study. Thewithaferin A was assayed through thin layer chromatography (TLC) method andphytochemical estimation was carried out by standard methodologies to detect the presence of secondary metabolites, like total phenol, flavonoids and anthocyanin. Our findings revealed that withaferin A was found in all accessions collected from different natural habits. The photochemical investigation showed the presence of flavonoids (5.70-6.50%), anthocyanin (4.51-9.51µmol/g) and total phenol (14.91-23.7µgGAE/mgD.W), varying significantly among the habitats (p<0.05). In the present study, TLC analysis confirms the exsistance of withaferin A in all W. coagulans accessions as well as the potential of Sistan and Baluchestan natural habits to produce this valuable component. However, the root extracts of W. coagulans, growing in natural habitats of Iran, have higher phenolic content. Hence, it can be used as a source of natural components for the pharmaceutical and food industries. | ||
| Keywords | ||
| Phytochemical investigation; Withania coagulans (Stocks) Dunal; Sistan and Baluchestan; withaferin A | ||
| References | ||
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Abouzid, S.F., El-Bassuony, A.A., Nasib, A., Khan, S., Qureshi, J. and Choudhary, M.I., 2010. Withaferin A production by root cultures of Withania coagulans. International Journal of Applied ResearchinNatural Products,3(1): 23-27. - Andre, C.M., Schafleitner, R., Leagy, S., Lefever, I., Aliaga, C.A.A., Nomberto, G., Hoffman, L., Hausman, J.F., Larondelle, Y. and Evers, D., 2009. Gene expression change related to the production of phenolic compounds in potato tubers grown under drought stress. Phytochemistry, 70(9): 1107-1116. - Arora, S., Dhillon, S., Rani, G. and Nagpal, A., 2004. The in vitro antibacterial/synergistic activities of Withania somnifera extracts. Fitoterapia, 75(3-4): 385-388. - Baldi, A., Singh, D. and Dixit, V.K., 2008. Dual elicitation for improved production of withaferin A by cell suspension cultures of Withania somnifera. Applied BiochemistryandBiotechnology, 151: 556-564. - Chitturi, D., Venisetty, R.K., Molmoori, R.K., Kokate, C.K. and Apte, S.S., 2010. Enhanced bioproduction of withaferin A from suspension cultures of Withania somnifera. Annals of Biological Research, 1(2): 77-86. - Golluce, M., Sahin, F., Sokmen, M., Ozer, H., Daferera, D., Sokmen, A., Polissiou, M., Adiguzel, A. and Ozken, H., 2007. Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. longifolia. Food Chemistry, 103: 1449-1456. - Krizek, D.T, Britz, S.J. and Mirecki, R.M., 1998. Inhibitory effects of ambient levels of solar UV-A and UV-B radiation on growth of cv. new red fire lettuce. Physiologia Plantarum, 103: 1-7. - Mahadevan, N., Kasar Rahul, P., Subburaju, T. and Suresh, B., 2003. HPTLC analysis of withaferine A from an herbal extract and polyherbal formulation. Journal of Separation Science, 26: 1707-1709. - Mansi, G., Deepa, B., Pandey Madan, M., Ojha Sanjeev, K., Sayyada, K., Subha, R. and Rawat Ajay, K.S., 2011. Standardization of Ashwagandhadi lehya-an important Ayurvedic formulation of Withania somnifera. Indian Journal of Traditional Knowledge, 10(4), 594-598. - Mathur, D., Agrawal, RC. and Shrivastava, V., 2011. Phytochemical screening and determination of antioxidant potential of fruits extracts of Withania coagulans. Recent Research in ScienceandTechnology, 3(11): 26-29. - Matta, A.J. and Giai, I., 1969. Accumulation of phenol in tomato plant is affected by different forms of Fusariumoxysporum. Planta Medica, 50: 512-513. - Mirjalili, M.H., Fakhr-Tabatabaei, S.M., Alizadeh, H., Ghassempour, A. and Mirzajani, F., 2009. Genetic and withaferin A analysis of Iranian natural populations of Withania somnifera and W. coagulans by RAPD and HPTLC. Natural Product Communications, 4: 337-346. - Mishra, L.C., Singh, B.B. and Dagenais, S., 2000. Scientific basis for therapeutic use of Withania somnifera (Ashwagandha): a review. Alternative Medicine Review, 5: 334-346. - Negi, M.S., Singh, A. and Lakshmikumaran, M., 2000. Genetic variation and relationship among and with in Withania species as revealed by AFLP markers. Genome, 43: 975-980. - Panwar, J. and Tarafdar, J.C., 2006. Distribution of three endangered medicinal plant species and their colonization with arbuscular mycorrhizal fungi. JournalofAridEnvironments, 5: 337-350. - Parihar, M.S. and Hemani, T., 2003. Phenolic antioxidants attenuate hippocampal neuronal cell damage against kainic acid induced exitoxicity. Journal of Biosciences, 28: 121-128. - Rahman, A., Shahwar, D., Naz, A. and Choudhary, M.I., 2003. Withanolides form Withania coagulans. Phytochemistry, 63(4): 387-390. - Rice-Evans, C.A., Miller, N.J. and Paganga, G., 1997. Antioxidant properties of phenolic compounds. TrendsinPlant Science, 2(4): 152-159. - Sakihama, Y., Mano, J., Sano, S., Asada, K. and Yamasaki, H., 2000. Reduction of phenoxyl radicals mediated by mono dehydroascorbate reductase. BiochemicalandBiophysical ResearchCommunications, 279(3): 949-954. - Salwan, C., Kumar, R. and Mann, A.S., 2012. Evolution of antioxidant activity of leaves of Withania coagulans Dunal. International JournalofPharmaand Bio Sciences, 1(1): 126-132. - Sangwan, R.S., Chaurasiya, N.D., Lal, P., Misra, L., Uniyal, G.C., Tuli, R. and Sangwan, N.S., 2007. Withanolide A biogeneration in in vitro shoot cultures of ashwagandha (Withania somnifera Dunal), a main medicinal plant in Ayurveda. Chemical and Pharmaceuticalbulletin, 55: 1371-1375. - Sangwan, R.S., Chauraslya, N.D., Misra, L.N., Lal, P., Uniyal, G.C. and Sangwan, N.S., 2005. An improved process for isolation of withaferin A from plant materials and products therefrom. US Patent 7, 108, 870. - Sharma, R.K., Samant, S.S., Sharma, P. and Devi, S., 2012. Evaluation of antioxidant activities of Withania somnifera leaves growing in natural habitats of north-west Himalaya, India. Journal of Medicinal Plants Research, 6(5): 657-661. - Singh, G. and Kumar, P., 2011. Evaluation of antimicrobial efficacy of flavonoids of Withania somnifera L. Indian Journalof Pharmaceutical Sciences, 73(4): 473-478. - Siriwadane, A.S., Dharmadasa, R.M. and Samarasinghe, K., 2013. Distribution of Withaferin A, an anticancer agent, in different parts of two varieties of Withania somnifera (L.) Dunnal. grown in Srilanka. Pakistan Journal of Biological Science, 16(3): 141-144. - Valizadeh, J. and Valizadeh, M., 2011. Development of efficient micropropagation protocol for Withania coagulans (Stocks) Dunal. African Journal of Biotechnology, 10(39): 7611-7616. - Wagner, G.J., 1979. Content and vacuole/extra vacuole distribution of neutral sugars, free amino acids, and anthocyanins in protoplast. Plant Physiology, 64: 88-93. | ||
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