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بررسی اثر ضدویروسی عصاره آبی گیاه سرخاب (Phytolacca americana L.) در کنترل ویروس وای سیبزمینی | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 10، دوره 31، شماره 3 - شماره پیاپی 71، مرداد 1394، صفحه 479-488 اصل مقاله (509.11 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2015.101898 | ||
| نویسندگان | ||
| اطهر علیشیری* 1؛ فرشاد رخشندهرو2 | ||
| 1دانشجوی کارشناسی ارشد رشته بیماریشناسی گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران | ||
| 2استاد، گروه بیماریشناسی گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران | ||
| چکیده | ||
| سرخاب یا علفپوک (pokeweed) (Phytolacca americana L.) گیاه بومی کشورهای مختلف قاره آمریکا بوده و با وجود احتمال سمّیت گوارشی، از توان کنترلکنندگی برخی عوامل بیماریزای انسانی و گیاهی برخوردار است. این ویژگی بهدلیل حضور پروتئین غیرفعالکننده ریبوزوم (RIPs) در این گیاه میباشد. در این تحقیق، اثر عصاره آبی برگ و ساقه گیاه سرخاب در کنترل ویروس وای سیبزمینی (Potato virus Y-PVY) که از مهمترین ویروسهای آلودهکننده سیبزمینی است، در گیاه مدل توتون مورد بررسی قرار گرفت و حضور پروتئین ضدویروسی گیاه سرخاب (PAP) در عصاره با استفاده از SDS-PAGE تأیید شد. نتایج آزمون سرولوژیکی الایزا (DAS-ELISA) حکایت از کاهش میانگین جذب سرولوژیکی چاهکهای مربوط به نمونههای تیمار شده با عصاره آبی سرخاب در مقایسه با چاهک شاهد (نمونههای آلوده به ویروس که با عصاره علف پوک تیمار نشدهاند) در آزمون الایزا داشت. نتایج نشان داد که عصاره آبی گیاه سرخاب در غلظت ppm14/0 قادر است میزان آلودگی ویروس وای سیبزمینی را در گیاهان آلوده کاهش دهد. بهطوری که بیشترین میزان کنترلکنندگی عصاره تا چهار روز بعد از تیمار توتونهای آلوده به ویروس با عصاره سرخاب مشاهده شد. | ||
| کلیدواژهها | ||
| گیاه سرخاب (Phytolacca americana L.)؛ عصاره آبی؛ اثر ضدویروسی؛ ویروس وای سیبزمینی | ||
| عنوان مقاله [English] | ||
| Study on the antiviral activity of aqueous pokeweed (Phytolacca Americana L.) extracts against Potato virus Y | ||
| نویسندگان [English] | ||
| A. Alishiri1؛ F. Rakhshandehroo2 | ||
| 1Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده [English] | ||
| Pokeweed (Phytolacca Americana L.) is a plant native to American countries which is widely used across the continent. Despite the probability of having digestive toxicity, this plant has an important role in many therapeutic applications for humans and plants. This plant has a capacity to control some plant and human pathogens due to the presence of ribosome-inactivating proteins (RIPs) in this plant. In this work, the antiviral effect of aqueous pokeweed leaf and stem aqueous extracts against Potato virus Y, one of the most important viruses infecting potatoes, was studied in tobacco model plants. The presence of the pokeweed antiviral protein (PAP) in the extract was confirmed by SDS-PAGE analysis. Results of serological assay (DAS-ELISA) showed lower absorbance values for the pokeweed treated samples as compared with non-treated, PVY-infected control plants, indicating lower levels of PVY infections in the treated plants. Results showed that the aqueous extract of pokeweed, at concentration of 0.14 ppm, had an inhibitory effect against PVY infection in tobacco model plants with a maximum controlling effect up to four days post-inoculation. | ||
| کلیدواژهها [English] | ||
| Pokeweed (Phytolacca americana L.), aqueous extract, antiviral activity, Potato virus Y | ||
| مراجع | ||
|
- کرمی، م.، شهابی مجد، ن.، سعیدنیا، س. و عمرانی، ن.، 1386. سمّیت کبدی عصاره آبی، متانولی و فراکسیونهای عصاره متانولی گیاه سرخاب به روش پرفیوژن کبدی در موش صحرایی نر. دانشگاه علوم پزشکی شهرکرد، 9(1): 9-1. - Agbafor, K.N. and Akubugwo, E.L., 2007. Hypocholesterolaemic effect of ethanolic extract of fresh leaves of cymbopogon citrates (lemongrass). African Journal of Biotechnology, 6(5): 596-598. - Chen, Z.C., White, R.F., Antoniw, J.F. and Lin, Q., 1991. Effect of pokeweed antiviral protein (PAP) on the infection of plant viruses. Journal of Plant Pathology, 40(4): 612-620. - Choi, K.W., Jeon, H.S., Jeong, H.S., Kim, M.K., Lee, K.H., Moon, Y.H. and Na, B.K., 1997. Expression vector for phytolacca antiviral protein and process for preparing transgenic plant transformed therewith. Patenten, US5633155A. - Clark, M.F. and Adams, A.N., 1977. Characterization of the microplate methods of enzyme linked immunosorbent assay (elisa) for the detection of plant viruses. Journal of General Virology, 34: 475-483. - Cowan, M.M., 1999. Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12(4): 564-582. - D'Cruz, O.J., Waurzyniak, B. and Uckun, F.M., 2004. Mucosal toxicity studies of a gel formulation of native pokeweed antiviral protein. Toxicol Pathology, 32(2): 211-212. - De Bokx, J.A. and Huttinga, H., 1981. Potato virus Y. Descriptions of Plant Viruses, No: 242, http://www.dpvweb.net/dpv/showdpv.php?dpvno=242. - Duggar, B.M. and Armstrong, J.K., 1925. The effect of treating the virus of tobacco mosaic with the juice of various plants. Annals of the Missouri Botanical Garden, 12: 359-366. - Duke, J.A., 2001. Handbook of Medicinal Herbs. CRC Press, Boca Raton, 896p. - Hernandez-Villegas, M.M., Borges-Argaez, R., Rodriguez-Vivas, R.I., Torres-Acosta, J.F., Mendez-Gonzalez, M. and Caceres-Farfan, M., 2011. Ovicidal and larvicidal activity of the crude extracts from Phytolacca icosandra against Haemonchus contortus. Veterinary Parasitology, 30(179): 100-106. - Hudak, K.A., Wang, P. and Tumer, N.E., 2000. A novel mechanism for inhibition of translation by pokeweed antiviral protein: depurination of the capped RNA template. RNA, 6(3): 369-380. - Ibrahim, D., Chahardehi, A.M. and Sulaiman, S.F., 2013. Effect of botanical extract of Urtica dioica on MRSA: structural degeneration study. Journal of Applied Biological Science, 7(3): 39-44. - Irvin, J.D., 1975. Purification and partial characterization of the antiviral protein from Phytolacca americana which inhibits eukaryotic protein synthesis. Archives of Biochemistry and Biophysics, 169(2): 522-528. - Irvin, J.D., Kelly, T. and Robertus, J.D., 1980. Purification and properties of a second antiviral protein from Phytolacca americana which inactivate eukaryotic ribosomes. Archives of Biochemistry and Biophysics, 200: 418-425. - Kang, B.C., Yeam, I. and Jahn, M., 2005. Genetics of plant virus resistance. Annual Review of Phytopathology, 43: 581-621. - Karami, M., Saeidnia, S., Shahabi majd, N., Ebrahimzadeh, M.A., Omrani, N. and Salarian, A., 2009. Anti-nociceptive activity of aqueous methanolic extracts of phytolacca americana growing in Iran. Iranian Journal of Pharmaceutical Research, 8(3): 223-226. - Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685. - Lodge, J.K., Kaniewski, W.K. and Tumer, N.E., 1993. Broad-spactrum virus resistance in transgenic plants expressing pokeweed antiviral protein. Proceedings of the National Academy of Sciences, 90: 7089-7093. - Maness, L., Goktepe, I., Hardy, B., Yu, J. and Ahmedna, M., 2011. Antiproliferative and apoptotic effects on Phytolacca americana extracts and their fractions on breast and colon cancer cells. Research Journal of Medicinal Plant, 6: 17-26. - Mansouri, S., Kutky, M. and Hudak, K.A., 2012. Pokeweed antiviral protein increases HIV-1 particle infectivity by activating the cellular mitogen activated protein kinase pathway. Plos one, 7(5): e36369. - Matsingou, T.C., Kapsokefalou, M. and Safiloglou, A., 2001. Aqueous infusions of Mediterranean herbs exhibits antioxidants activity towards iron-promoted oxidation of phospholipids, linoleic acid and deoxyribose. Free Radical Research, 35(5): 593-605. - Modarresi-Chahardehi, A., Ibrahim, D., Fariza-Sulaiman, S. and Mousavi, L., 2012. Screening antimicrobial activity of various extracts of Urtica dioica. Revista de Biologia Tropical, 60(4): 1567-1576. - Moon, Y.H., Song, S.K., Choi, K.W. and Lee, J.S., 1997. Expression of a cDNA encoding Phytolacca insularis antiviral protein confers virus resistance of transgenic potato plants. Journal of Molecules and Cells 7: 807-815. - Newall, C.A., Anderson, L.A. and Phillipson, J.D., 1996. Herbal Medicine: A Guide for Health-Care Professional. Pharmaceutical Press, London 296p. - Ormeno, J., Sepulveda, P., Rojas, R. and Araya, J.E., 2006. Datura genus weeds as an epidemiological factor of Alfalfa mosaic virus (AMV), Cucumber mosaic virus (CMV), and Potato virus Y (PVY) on solanaceus crops. Agricultura Tecnica (CHILE), 66(4): 333-341. - Rakhshandehroo, F., Modarresi, A. and Zamani Zadeh, H.R., 2009. Study on the antiviral effect of aquatic and alcoholic extracts of Urtica dioica L. on rose mosaic viral disease in vitro culture. Iranian Journal of Medicinal and Aromatic Plants, 25(3): 403-413. - Spetz, C., Taboada, A.M., Drwich, S., Ramsell, J., Salazar, L.F. and Valkonen, J.P.T., 2003. Molecular resolution of a complex of potyviruses infecting solanaceus crops at the centre of origin in Peru. Journal of General Virology, 84: 2565-2578. - Tomlinson, J.A., Walker, V.M., Flewett, T.H. and Backlay, G.R., 1974. The inhibition of infection by cucumber mosaic virus and influenza virus by extracts from Phytolacca americana. Journal of General Virology, 22: 225-232. - Taylor, S., Massiah, A., Lomonossoff, G., Roberts, L.M., Lord, J.M. and Hartley, M., 1994. Correlation between the activities of five ribosome-inactivating proteins in depurination of tobacco ribo-somes and inhibition of tobacco mosaic virus infection. The Plant Journal, 5(6): 827-835. - Wyatt, S.D. and Shepherd, R.J., 1969. Isolation and characterization of a virus inhibitor from Phytolacca americana. Phytopathology, 59: 1787-1794 | ||
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