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GENETIC VARIATION ANALYSIS OF DIFFERENT POPULATIONS OF ROSA DAMASCENA IN NW. IRAN USING RAPD MARKERS | ||
| The Iranian Journal of Botany | ||
| مقاله 3، دوره 12، شماره 2، اسفند 2006، صفحه 121-127 اصل مقاله (302.39 K) | ||
| نوع مقاله: Research Paper | ||
| نویسندگان | ||
| Seyed Reza Tabaei-Aghdaei1؛ Hossein Hosseini Monfared2؛ Hamid Fahimi3؛ Hassan Ebrahimzade3؛ Maryam Jebelly1؛ Mohammad Reza Naghavi4؛ Alireza Babaei5 | ||
| 1Biotechnology Research Department of Natural Resources, Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran. | ||
| 2Zanjan Province Natural Resources Office, Zanjan, Iran. | ||
| 3Depaetment of Biology, Tehran University, Tehran, Iran. | ||
| 4Department of Plant Breeding, Tehran University, Tehran, Iran. | ||
| 5Department of Horticultural Science, Tabiat Modares University(TMU), Tehran, Iran. | ||
| چکیده | ||
| Random amplified polymorphic DNA (RAPD) markers have been used to characterize the genetic diversity among 12 Damask rose landraces from northwest of Iran. The 22 primers used in this study amplified 262 scorable RAPD loci among which 180 were polymorphic (67%). The average number of bands was 11.9 for each primer. Genetic similarity calculated from the RAPD data ranged from 0.261 to 0.995 with an average of 0.63. A dendrogram was prepared on the basis of a similarity matrix using the UPGMA algorithm and separated the 12 landraces into two groups. There was a good correspondence between genetic divergence and climate condition, though some landraces (such as L2 and L3) with diverse geographical condition were clustered in one group. The results showed a possible application of RAPD markers in assessment of genetic diversity in Rosa damascena landraces. | ||
| کلیدواژه‌ها | ||
| Rosa damascena Mill؛ genetic similarity؛ RAPD analysis | ||
| عنوان مقاله [English] | ||
| بررسی تنوع ژنتیکی جمعیت های مختلف گل محمدی شمال غرب ایران با استفاده از نشانگر RAPD | ||
| نویسندگان [English] | ||
| سید رضا طبائی عقدائی1؛ حسین حسینی منفرد2؛ حمید فهیمی3؛ حسن ابراهیم زاده3؛ مریم جبلی1؛ محمدرضا نقوی4؛ علیرضا بابائی5 | ||
| 1گروه تحقیقات بیوتکنولوژی موسسه تحقیقات جنگلها و مراتع، تهران، ایران | ||
| 2منابع طبیعی زنجان، زنجان، ایران | ||
| 3دانشگاه تهران، تهران، ایران | ||
| 4گروه اصلاح نباتات، دانشگاه تهران، تهران، ایران. | ||
| 5گروه علوم باغبانی، دانشگاه تربیت مدرس، تهران، ایران. | ||
| چکیده [English] | ||
| در این پژوهش نشانگر مولکولیRAPD برای تعیین تنوع ژنتیکی 12 جمعیت گل محمدی شمال غرب ایران مورد استفاده قرار گرفت. با استفاده از 22 آغازگر، تعداد 262 باند قابل تشخیص ایجاد شد که 67% آنها ( 180 باند) در بین جمعیتهای مختلف چند شکلی نشان دادند. تعداد متوسط باندها به ازای هر آغازگر 9/11 بود. شاخص تشابه Dice برای تعیین همسانیهای ژنتیکی بین جمعیتها مورد استفاده قرار گرفته و با استفاده از الگوریتم UPGMA یک دندروگرام بر مبنای ماتریس تشابه تهیه شد که 12 جمعیت گل محمدی را در2 گروه مختلف قرار داد. نتایج بدست آمده ارتباط خوبی بین تنوع آب و هوایی و مولکولی نشان دادند، گرچه جمعیتهای با تنوع جغرافیایی (مانند L2 وL3) نیز در یک گروه قرار گرفتند. نتایج این پژوهش بر امکان بکارگیری نشانگرهای RAPD در ارزیابی تنوع ژنتیکی ژرم پلاسم گل محمدی تاکید داشت. | ||
| کلیدواژه‌ها [English] | ||
| Rosa damascena Mill, genetic similarity, RAPD analysis | ||
| مراجع | ||
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Achuthan, C.R., Babu, B.H. & Padikkala, J., 2003: Antioxidant and hepatoprotective effects of Rosa damascena. Pharmaceutical Biology. 41: 357-361. Agaoglu, Y., Ergul, A. & Baydar, N. 2000: Molecular analyses of genetic diversity of oil rose (Rosa damascena Mill.) grown in Isparta (Turkey) region. Biotechnol Biotechnol Eq. 14:16-18. Ardogan, B.C., Baydar, H., Kaya, S., Demirci, M., Ozbasar, D. & Mumcu, E. 2002: Antimicrobial activity and chemical composition of some essential oils. Archives-of-Pharmacal-Research. 25: 860-864. Ballard, R., Rajapakse, S., Abbott, A. & Byrne, D. 1995: DNA markers in rose and their use for cultivar identification and genome mapping. Acta Hortic. 424:265–268. Basim, E. & Basim H. 2003: Antibacterial activity of Rosa damascena essential oil. Fitoterapia. 74:394-396. Baydar, N.G., Baydar, H. & Debener, T. 2004: Analysis of genetic relationships among Rosa damascena plants grown in Turkey by using AFLP and microsatellite markers. J. Biotech. 111:263-267. Belaj, A., Satovic, Z., Cipriani, G., Baldoni, L., Testolin, R., Rallo, L. & Trujillo, I. 2003: Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive. Theor Appl Genet. 107: 736-744. Chevallier, A. 1996: The encyclopedia of medicinal plants. Dorling Kindersely, London, 336 pp. Debener, T. & Mattiesch, L. 1999: Construction of a genetic linkage map for roses using RAPD and AFLP markers. Theor Appl Genet. 99: 891–899. Debener, T., Bartels, C. & Mattiesch, L. 1996: RAPD analysis of genetic variation between a group of rose cultivars and selected wild rose species. Mol Breed 119:71-74. Debener, T., Janakiram, T. & Mattiesch, L. 2000: Sports and seedlings of rose varieties analysed with molecular markers. Plant Breeding. 119:71–74. Esselink, D., Nybom, H. & Vosman, B. 2004: Assignment of allelic configuration in polyploids using the MAC-PR (Microsatellite DNA Allele Counting–Peak Ratios) Method. Theor Appl Genet. 109:402–408. Esselink, GD., Smulders, MJM. & Vosman, B. 2003: Identification of cut rose (Rosa hybrida) rootstocks varieties using robust sequence tagged microsatellite site markers. Theor Appl Genet. 106:277–286. Gudin, S. 2000: Rose: genetics and breeding. Plant Breed. Rev. 17:59-189. Huff, DR., Peakall, R. & Smouse, PE. 1993: RAPD variation within and among natural populations of outcrossing buffalo-grass [Buchloe dactyloides (Nutt.) Engelm. Theor Appl Genet. 86:927–934. Jan, CH., Byrne DH, Manhart J. & Wilson H. 1999: Rose germplasm analysis with RAPD markers. Hortscience 34: 206-209 Jones, CJ., Edwards, KJ., Castaglione, S., Winfield, MO., Sala, F., van de Wiel, C., Bredemeijer, G., Vosman, B., Matthes, M., Daly, A., Brettschneider, R., Bettini, P., Buiatti, M., Maestri, E., Malcevschi, A., Marmiroli, N., Aert, R., Volckaert, G., Rueda, J., Linacero, R., Vazquez, A. & Karp, A. 1997: Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories. Molecular Breeding. 3: 381-390. Mahmood, N., Piacente, S., Pizza, C., Burke, A., Khan, A.I. & Hay, A.J. 1996: The Anti-HIV Activity and Mechanisms of Action of Pure Compounds Isolated from Rosa damascena. Bioch. Biophys. Res. Comm. 220:73-79. Milbourne, D., Meyer, R., Bradshaw, J.E., Baird, E., Bonar, N., Provan, J., Powell, W. & Waugh R. 1997: Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato. Mol. Breeding 3: 127–136. Naghavi, M.R., Mardi, M., Ramshini, H.A. & Fazelinasab, B. 2004: Comparative analyses of the genetic diversity among bread wheat genotypes based on RAPD and SSR markers. Iranian J. Biotechnology, 2: 195-202. Nei, M. & Li, W.H. 1979: Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA. 76: 5269-5273. Ozkan, G., Sagdic, O., Baydar, N.G. & Baydar, H. 2004: Antioxidant and antibacterial activities of Rosa damascena flower extracts. Food Sci. Technol. Int.10: 277-281. Rajapakse, S., Hubbard, M., Kelly, J., Abbott , A. & Ballard, R. 1992: Identification of rose cultivars by restriction fragment length polymorphism. Sci Hort. 52:237–245. Rohlf, F.J. 1998: NTSYS-PC. Numerical taxonomy and multivariate analysis system, version 2.00. Exeter Software, Setauket, NY. Rusanov, R., Kovacheva, N., Vosman, B., Zhang, L., Rajapakse, S., Atanassov, A. & Atanassov, I. 2005: Microsatellite analysis of Rosa damascena Mill. Accessions reveal genetic similarity between genotypes used for rose oil production and oil Damask rose varieties. Theor. Appl. Genet. 111:804-809. Russell, JR., Fuller, JD., Macaulay, M., Hatz, BG., Jahoor, A., Powell, W. & Waugh, R. 1997: Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs. Theor Appl Genet. 95: 714–722. Welsh, J. & McClelland, M. 1990: Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18: 7213–7218. Williams, JGK., Kubelik, AR., Livak, KJ., Rafalski, JA. & Tingey, SV. 1990: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531–6535. | ||
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