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A Study on molecular characterization of Razi Bacillus anthracis Sterne 34F2 substrain in Iran | ||
| Archives of Razi Institute | ||
| مقاله 2، دوره 71، شماره 2، شهریور 2016، صفحه 81-86 اصل مقاله (117.18 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22034/ari.2016.106445 | ||
| نویسندگان | ||
| K. Tadayon* ؛ G. Moazeni Jula؛ R. Banihashemi؛ M. Sekhavati؛ A.A. Naseri Rad؛ H. Razaz | ||
| Department of Veterinary Aerobic Bacteria, Razi Vaccine and Serum Research Institute,Education and Extension Organization, Karaj, Iran | ||
| چکیده | ||
| Anthrax, a zoonotic disease caused by Bacillus anthracis, has affected humans since ancient times. For genomic characterization of Razi B. anthracis Sterne 34F2 substrain, single nucleotide polymorphism (SNP) genotyping method developed by Van Erth, variable-number tandem-repeat (VNTR)-8 analysis proposed by Keim, and multiple-locus VNTR analysis (MLVA)-3 introduced by Levy were employed. In the SNPs typing system, where the nucleotide content of the genome at 13 evolutionary canonical loci was collectively analyzed, the originally South African 34F2 substrain was categorized in the A.Br.001/002 subgroup. In the VNTR-8 analysis, fragments with lengths of 314, 229, 162, 580, 532, 158, and 137 bp were identified at the following loci: vrrA, vrrB1, vrrB2, vrrC1, vrrC2, CG3, and pxO1, respectively. In addition, application of Levy's MLVA-3 genotyping method revealed that the genome of this strain carried 941, 451, and 864 bp fragments at AA03, AJ03, and AA07 loci, respectively. The present findings are undoubtedly helpful in meeting the requirements set by the World Organization for Animal Health (OIE) and World Health Organization (WHO) for anthrax vaccine manufacturers including Razi Institute. However, further similar studies are required to promote the current epidemiological knowledge of anthrax in Iran. | ||
| کلیدواژه‌ها | ||
| Iran؛ Anthrax؛ Max Sterne | ||
| عنوان مقاله [English] | ||
| تعیین خصوصیات ژنتیکی باسیلوس آنتراسیس استرن F2 34 تحت سویه رازی | ||
| نویسندگان [English] | ||
| کیوان تدین؛ غلامرضا موذنی جولا؛ رضا بنی هاشمی؛ محمد سخاوتی؛ علی اکبر ناصری راد؛ حسین رزاز | ||
| بخش تحقیق و تولید واکسن‌های هوازی دامی، موسسه تحقیقات واکسن و سرم سازی رازی، کرج، ایران | ||
| چکیده [English] | ||
| آنتراکس به عنوان یک بیماری مشترک با عامل باسیلوس آنتراسیس شناخته میشود از گذشته دور سبب ایجاد بیماری در انسان می‌گردیده است. به منظور مشخص نمودن ویژگی‌های ژنتیکی ژنوم تحت سویه رازی Bacillus anthracis 34F2 روش SNP تایپینگ تکمیل شده توسط van Erth و روش تایپینگ VNTR 8 ابداعی Keim و همچنین روش MLVA 3 معرفی شده توسط Levy مورد استفاده قرار گرفتند. در سیستم SNP typing که ساختار نوکلئوتید های ژنوم باکتری در 13 لوکوس کانونی مهم از نظر تکاملی بصورت تجمیعی مورد آنالیز قرار می گیرند٬ تحت سویه Bacillus anthracis 34F2 که دارای اصالت آفریقای جنوبی می باشد در گروه A.Br.001/002 قرار گرفت. در VNTR 8 قطعاتی به طول 314 و 229 و 162 و 580 و 532 و 158و 137 زوج باز در ژنوم این سویه در لوکوس های vrrA, vrrB1, vrrB2 ,vrrC1, vrrC2, CG3 and pxO1 شناسایی گردیدند. علاوه بر این در جریان استفاده از روش ژنوتایپینگ Levy که مبتنی بر سه لوکوس میباشد وجود قطعاتی به طول 941 و 451 و 864 به ترتیب در مورد لوکوس های AA03, AJ03 , AA07 مورد توجه قرار گرفتند. بدون تردید یافته‌های این تحقیق در تامین الزامات سازمانهای بین‌المللی OIE و WHO که ناظر بر عملکرد تولیدکنندگان فرآورده‌های بیولوژیک و از جمله مؤسسه رازی می‌باشند مفید و کاربردی می‌باشند. با این حال انتظار میرود با انجام مطالعات مشابه دانش حال حاضر موجود از اپیدمیولوژی آنتراکس در ایران بهبود یابد. | ||
| کلیدواژه‌ها [English] | ||
| ایران, آنتراکس, ماکس استرن | ||
| مراجع | ||
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Behr, M., Small, P., 1999. A historical and molecular phylogeny of BCG strains. Vaccine 17, 915-922. Delpi, L.P., 1938. Infectious diseases of animals in Study on infectious diseases of farm animals in iran, Razi Institute, Karaj. Feodorova, V.A., Sayapina, L.V., Corbel, M.J., Motin, V.L., 2014. Russian vaccines against especially dangerous bacterial pathogens. Emerging microbes & infections 3, e86. Gilfoyle, D., 2006. Anthrax in South Africa: economics, experiment and the mass vaccination of animals, c. 1910–1945. Medical history 50, 465-490. Honda, I., Seki, M., Ikeda, N., Yamamoto, S., Yano, I., Koyama, A., Toida, I., 2006. Identification of two subpopulations of Bacillus Calmette-Guerin (BCG) Tokyo172 substrain with different RD16 regions. Vaccine 24, 4969-4974. Jula, G.M., Sattari, M., Banihashemi, R., Razzaz, H., Sanchouli, A., Tadayon, K., 2011. The phenotypic and genotypic characterization of Bacillus anthracis isolates from Iran. Tropical animal health and production 43, 699-704. Keim, P., Price, L., Klevytska, A., Smith, K., Schupp, J., Okinaka, R., Jackson, P., Hugh-Jones, M., 2000. Multiple-locus variable-number tandem repeat analysis reveals genetic relationships within Bacillus anthracis. Journal of Bacteriology 182, 2928-2936. Legge, T., 1905. The milroy lectures on industrial anthrax: Delivered before the royal college of physicians of london. British Medical Journal 1, 589. Levy, H., Fisher, M., Ariel, N., Altboum, Z., Kobiler, D., 2005. Identification of strain specific markers in Bacillus anthracis by random amplification of polymorphic DNA. FEMS Microbiology Letters 244, 199-205. Najafi Olya, Z., Tadayon, K., Ghaderi, R., 2015. A Simplified Van Erth SNP- Typing Method of Bacillus Anthracis Applicable by Traditional Thermocycler Machines. Medical Laboratory Journal 9, 97-103. Oettinger, T., Jørgensen, M., Ladefoged, A., Hasløv, K., Andersen, P., 1999. Development of the Mycobacterium bovis BCG vaccine: review of the historical and biochemical evidence for a genealogical tree. Tubercle and lung disease 79, 243-250. Popa, V., Manea, M., Pastrama, F., Burlacu, O., Voinovschi, E., Botus, D., Danes, M., 2009. Genetic characterization of bacillus anthracis R1190 stamatin vaccinal strain. Lucrari Stiintifice-Universitatea de Stiinte Agricole a Banatului Timisoara, Medicina Veterinara 42, 299-303. Seyyed-Mohammadi, S., Bidhendi, S.M., Tadayon, K., Ghaderi, R., 2015. Genetic Characterization of Bacillus anthracis 17 JB strain. Iranian Journal of Microbiology 7, 168-172. Tigertt, W.D., 1980. Anthrax. William Smith Greenfield, M.D., F.R.C.P., Professor Superintendent, the Brown Animal Sanatory Institution (1878-81). Concerning the priority due to him for the production of the first vaccine against anthrax. The Journal of hygiene 85, 415-420. Turnbull, P.C., 1991. Anthrax vaccines: past, present and future. Vaccine 9, 533-539. Van Ert, M.N., Easterday, W.R., Huynh, L.Y., Okinaka, R.T., Hugh-Jones, M.E., Ravel, J., 2007. Global genetic population structure of Bacillus anthracis. PloS one 2, e461. Wang, Z., Pan, Y., Wu, J., Zhu, B., 2012. [Complete genome sequencing and sequence analysis of BCG Tice]. Wei sheng wu xue bao = Acta microbiologica Sinica 52, 1219-1227. Xu, D., Li, G., Wu, L., Zhou, J., Xu, Y., 2002. PRIMEGENS: robust and efficient design of gene-specific probes for microarray analysis. Bioinformatics 18, 1432-1437. Behr, M., Small, P., 1999. A historical and molecular phylogeny of BCG strains. Vaccine 17, 915-922. Delpi, L.P., 1938. Infectious diseases of animals in Study on infectious diseases of farm animals in iran, Razi Institute, Karaj. Feodorova, V.A., Sayapina, L.V., Corbel, M.J., Motin, V.L., 2014. Russian vaccines against especially dangerous bacterial pathogens. Emerg Microbes Infect 3, e86. Gilfoyle, D., 2006. Anthrax in South Africa: economics, experiment and the mass vaccination of animals, c. 1910–1945. Medical history 50, 465-490. Honda, I., Seki, M., Ikeda, N., Yamamoto, S., Yano, I., Koyama, A., Toida, I., 2006. Identification of two subpopulations of Bacillus Calmette-Guerin (BCG) Tokyo172 substrain with different RD16 regions. Vaccine 24, 4969-4974. Jula, G.M., Sattari, M., Banihashemi, R., Razzaz, H., Sanchouli, A., Tadayon, K., 2011. The phenotypic and genotypic characterization of Bacillus anthracis isolates from Iran. Trop Anim Health Prod 43, 699-704. Keim, P., Price, L., Klevytska, A., Smith, K., Schupp, J., Okinaka, R., Jackson, P., Hugh-Jones, M., 2000. Multiple-locus variable-number tandem repeat analysis reveals genetic relationships within Bacillus anthracis. Journal of Bacteriology 182, 2928-2936. Legge, T., 1905. The milroy lectures on industrial anthrax: Delivered before the royal college of physicians of london. British Medical Journal 1, 589. Levy, H., Fisher, M., Ariel, N., Altboum, Z., Kobiler, D., 2005. Identification of strain specific markers in Bacillus anthracis by random amplification of polymorphic DNA. FEMS Microbiology Letters 244, 199-205. Najafi Olya, Z., Tadayon, K., Ghaderi, R., 2015. A Simplified Van Erth SNP- Typing Method of Bacillus Anthracis Applicable by Traditional Thermocycler Machines. Medical Laboratory Journal 9, 97-103. Oettinger, T., Jørgensen, M., Ladefoged, A., Hasløv, K., Andersen, P., 1999. Development of the Mycobacterium bovis BCG vaccine: review of the historical and biochemical evidence for a genealogical tree. Tubercle and lung disease 79, 243-250. Popa, V., Manea, M., Pastrama, F., Burlacu, O., Voinovschi, E., Botus, D., Danes, M., 2009. Genetic characterization of bacillus anthracis R1190 stamatin vaccinal strain. Lucrari Stiintifice-Universitatea de Stiinte Agricole a Banatului Timisoara, Medicina Veterinara 42, 299-303. Seyyed-Mohammadi, S., Bidhendi, S.M., Tadayon, K., Ghaderi, R., 2015. Genetic Characterization of Bacillus anthracis 17 JB strain. Iranian Journal of Microbiology 7, 168-172. Tigertt, W.D., 1980. Anthrax. William Smith Greenfield, M.D., F.R.C.P., Professor Superintendent, the Brown Animal Sanatory Institution (1878-81). Concerning the priority due to him for the production of the first vaccine against anthrax. Journal of Hygiene (London) 85, 415-420. Turnbull, P.C., 1991. Anthrax vaccines: past, present and future. Vaccine 9, 533-539. Van Ert, M.N., Easterday, W.R., Huynh, L.Y., Okinaka, R.T., Hugh-Jones, M.E., Ravel, J., 2007. Global genetic population structure of Bacillus anthracis. PLoS One 2, e461. Wang, Z., Pan, Y., Wu, J., Zhu, B., 2012. [Complete genome sequencing and sequence analysis of BCG Tice]. Wei Sheng Wu Xue Bao 52, 1219-1227. Xu, D., Li, G., Wu, L., Zhou, J., Xu, Y., 2002. PRIMEGENS: robust and efficient design of gene-specific probes for microarray analysis. Bioinformatics 18, 1432-1437.
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