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القای درون‌شیشه‌ای تتراپلوئیدی در گیاه دارویی کتان سفید (Linum album Kotschy ex Boiss.) در تیمار اوریزالین | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 15، دوره 32، شماره 2 - شماره پیاپی 76، خرداد 1395، صفحه 346-359 اصل مقاله (351.62 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2016.106568 | ||
| نویسندگان | ||
| ندا جوادیان1؛ قاسم کریم‌زاده* 2؛ مظفر شریفی3؛ احمد معینی2؛ ژاله تحصیلی4 | ||
| 1دانشجو دکتری، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران | ||
| 2دانشیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران | ||
| 3دانشیار، گروه زیست‌شناسی گیاهی، دانشکده علوم پایه، دانشگاه تربیت مدرس، تهران، ایران | ||
| 4دانش‌آموخته دکتری، گروه زیست‌شناسی گیاهی، دانشکده علوم پایه، دانشگاه تربیت مدرس، تهران، ایران | ||
| چکیده | ||
| کتان سفید (Linum album Kotschy ex Boiss.) گیاهی چندساله و دیپلوئید (26=x2=n2) و یکی از گونه‌های بومی ایران و از خانواده کتان (Linaceace) است که دارای ماده مؤثره پودوفیلوتوکسین می‌باشد. این ماده یک محصول طبیعی مهم دارویی است که به گروه شیمیایی لیگنان‌ها تعلق دارد. به‌عنوان یک پیش‌ماده برای سنتز داروهای ضدسرطانی استفاده می‌شود. تولید صنعتی این ترکیب به‌دلیل ساختار شیمیایی پیچیده، از لحاظ اقتصادی امکان‌پذیر نیست. بنابراین، از روش‌های مختلفی برای افزایش تولید پودوفیلوتوکسین در گیاه استفاده می‌شود. در این تحقیق، القای تتراپلوئیدی با استفاده از غلظت‌های متفاوت اوریزالین 0، 15، 25 و µM 50 به مدت 24، 48، 72 و 96 ساعت روی جوانه جانبی این گیاه انجام شد. نتایج نشان داد درصد زنده‌مانی و تعداد تولید نوساقه در هر جوانه جانبی در این گیاه با افزایش غلظت‌ها و زمان‌های اوریزالین کاهش یافت. گیاهان تتراپلوئید به‌وسیله آنالیز فلوسایتومتری شناسایی شدند، همچنین مقدار C DNA2 برای گیاهان دیپلوئید و تتراپلوئید به‌ترتیب 34/4 و pg36/8 برآورد شد. بیشترین درصد بازدهی القای تتراپلوئیدی (19%) تحت تیمار اوریزالین در غلظت µM25 در 48 ساعت بدست آمد. القای تتراپلوئیدی باعث افزایش مقدار پودوفیلوتوکسین در برگ و ساقه تتراپلوئید نسبت به دیپلوئید خود به‌ترتیب 66/1 و 48/1 برابر شد. نتایج این تحقیق نشان داد که القای تتراپلوئیدی می‌تواند روش مؤثری برای افزایش این ماده ارزشمند در گیاه کتان سفید باشد. | ||
| کلیدواژه‌ها | ||
| کتان سفید (Linum album Kotschy ex Boiss.)؛ تتراپلوئیدی؛ اوریزالین؛ پودوفیلوتوکسین؛ 2C DNA؛ فلوسایتومتری | ||
| عنوان مقاله [English] | ||
| In vitro induction of tetraploidy in Linum album Kotschy ex Boiss. using oryzalin treatment | ||
| نویسندگان [English] | ||
| N. Javadian1؛ Gh. Karimzadeh2؛ M. Sharifi3؛ A. Moeini2؛ Zh. Tahsili4 | ||
| 1Ph.D. Student, Plant Breeding and Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| 2Plant Breeding and Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| 3Plant Biology Department, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran | ||
| 4Plant Biology Department, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran | ||
| چکیده [English] | ||
| Linum album Kotschy ex Boiss. is one of the endemic species of Linaceace family in Iran, having podophyllotoxin (PTOX) metabolite. This substance is a pharmaceutically important natural product belonging to the lignans group. It is used as a precursor for the synthesis of important anti-cancer drugs. Industrial production of this component is not economically feasible because of its complex chemical structure. Therefore, various strategies have been used to increase the production of PTOX in plants. In this study, tetraploidy was induced in nodal segments with different concentrations of oryzalin (0, 15, 25, 50 µM) for 24, 48, 72 and 96 h. The results indicated that the viability and the number of produced shoots in each nodal segment were reduced by increasing concentrations and times of oryzalin. Tetraploid plants were identified by flow cytometry, and 2C DNA content of diploids and tetraploids were estimated to be 4.34 and 8.36 pg, respectively. The highest induction efficiency percentage (19%) of tetraploidy was achieved from 25 µM of oryzalin for 48 h. Tetraploid induction caused 1.66 and 1.48 fold-increase in PTOX content in the leaves and stems of tetraploids compared with those of diploids, respectively. The results of this study indicated that the induction of tetraploidy could be an effective technique to increase this valuable metabolite in Linum album. | ||
| کلیدواژه‌ها [English] | ||
| Linum album Kotschy ex Boiss, TETRAPLOIDY, oryzalin, Podophyllotoxin, 2C DNA, Flow cytometry | ||
| مراجع | ||
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