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Prevalence and phylogenetic analysis of Theileria equi in Iranian dromedaries | ||
| Archives of Razi Institute | ||
| مقاله 4، دوره 71، شماره 3، آذر 2016، صفحه 169-175 اصل مقاله (273.89 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22034/ari.2016.106970 | ||
| نویسندگان | ||
| S. Bahrami* 1؛ M.R. Tabandeh2؛ A. Nikbin1؛ A.R. Alborzi1؛ A.R. Ghadrdan3 | ||
| 1Department of Parasitology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 2Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 3Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| چکیده | ||
| Considering the importance of Theileria equi infection in horse breeding industry and marketing, in the present study, we aimed to determine the prevalence of T. equi among dromedaries in central Iran, where a considerable number of camels and horses are raised and equine theileriosis is quite prevalent. For this purpose, a total of 161 blood samples from camels were examined in terms of T. equi infection, using parasitological and molecular methods. For molecular detection of T. equi, primers targeting the 18S rRNA gene were selected. Microscopic examination revealed that 0.6% of camels were positive for the intraerythrocytic stage of Theileria species, while polymerase chain reaction (PCR) method detected T. equi in 7 (4.3%) out of 161 camels. Sequences of 18S rRNAs from all the isolates showed more than 99% homology to each other and T. equi isolates in the GenBank. With respect to the single-nucleotide substitution in 18S rRNA gene of the studied camels, three different genotypes were identified and submitted to the GenBank. Considering the homology between 18S rRNA sequences of T. equi in the studied samples and those available in the GenBank, the phylogenetic tree formed three distinct, but highly-related clusters. In this study, age, gender, and locality were not determined as risk factors for T. equi infection in camels. In conclusion, this study demonstrated that T. equi is present among Iranian camels. | ||
| کلیدواژهها | ||
| Theileria equi؛ Camel؛ Polymerase Chain Reaction؛ Phylogenetic analysis؛ Yazd | ||
| عنوان مقاله [English] | ||
| شناسایی مولکولی و آنالیز فیلوژنیک تیلریا اکوئی در شترهای یزد (Camelus dromedarius) | ||
| نویسندگان [English] | ||
| سمیه بهرامی1؛ محمدرضا تابنده2؛ علی نیکبین1؛ علیرضا البرزی1؛ علیرضا قدردان3 | ||
| 1گروه انگل شناسی، دانشکده دامپزشکی، دانشگاه شهید چمران، اهواز، ایران | ||
| 2گروه بیو شیمی و مولکولار بیولوژی، دانشکده دامپزشکی، دانشگاه شهید چمران، اهواز، ایران | ||
| 3گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران، اهواز، ایران | ||
| چکیده [English] | ||
| با توجه به اهمیت عفونت تیلریا اکویی در صنعت تولید و صادرات اسب، هدف از مطالعه حاضر بررسی حضور تیلریا اکویی در شترهای مرکز ایران، مکانی که در آن جا جمعیت قابل توجهی از شتر و اسب وجود دارد و نیز تیلریوز اسبی شایع است بوده است. در مجموع نمونه خون 161 شتر به روش انگلشناسی و مولکولی برای بررسی حضور انگل تیلریا اکوئی بررسی گردید. برای جستجوی تیلریا اکوئی، پرایمرهایی که هدف آنها ژن 18s rRNA بود انتخاب گردید. مطالعهی میکروسکوپیک نشان داد که 6/0 درصد شترها برای مرحله درون گلبول قرمزی انگل تیلریا مثبت بودهاند در حالی که از 161 نمونه، 7 نمونه (3/4%) با استفاده از روش واکنش زنجیرهای پلیمراز، آلوده به تیلریا اکویی تشخیص داده شد. توالی ژن 18s rRNA تمام جدایهها، بیش از 99 درصد شباهت با یکدیگر و تیلریا اکوییجدا شده از اسبها و شترهای مطالعات دیگر داشته است. بر اساس جایگزینی تک نوکلئوتیدی در ژن 18s rRNA از شترهای مورد مطالعه، 3 ژنوتیپ مختلف شناسایی و به بانک ژن ارائه شد. بر اساس همولوژیهای بین توالی 18s rRNA تیلریا اکویی به دست آمده از شترها و اسبهای مطالعه و ارائه شده به بانک ژن، درخت فیلوژنی 3 شاخه بسیار مرتبط مجزا تشکیل داد. سن، جنس و محل جغرافیایی به عنوان عوامل خطر آلودگی به تیلریا اکویی در شترهای این مطالعه مشخص نشد. در پایان، این مطالعه نشان داد که در حال حاضر شترهای ایران به تیلریا اکویی آلوده هستند | ||
| کلیدواژهها [English] | ||
| تیلریا اکوئی, واکنش زنجیرهای پلیمراز, آنالیز فیلوژنیک, یزد | ||
| مراجع | ||
|
Abedi, V., Razmi, G., Seifi, H., Naghibi, A., 2014. Molecular and serological detection of Theileria equi and Babesia caballi infection in horses and ixodid ticks in Iran. Ticks Tick Borne Dis 5, 239-244. Acici, M., Umur, S., Guvenc, T., Arslan, H.H., Kurt, M., 2008. Seroprevalence of equine babesiosis in the Black Sea region of Turkey. Parasitol Int 57, 198-200. Alhassan, A., Govind, Y., Tam, N.T., Thekisoe, O.M., Yokoyama, N., Inoue, N., Igarashi, I., 2007. Comparative evaluation of the sensitivity of LAMP, PCR and in vitro culture methods for the diagnosis of equine piroplasmosis. Parasitol Res 100, 1165-1168. Alhassan, A., Pumidonming, W., Okamura, M., Hirata, H., Battsetseg, B., Fujisaki, K., Yokoyama, N., Igarashi, I., 2005. Development of a single-round and multiplex PCR method for the simultaneous detection of Babesia caballi and Babesia equi in horse blood. Vet Parasitol 129, 43-49. Apanaskevich, D.A., Schuster, A.L., Horak, I.G., 2008. The Genus Hyalomma: VII. Redescription of all Parasitic Stages of H. (Euhyalomma) dromedarii and H. (E.) schulzei (Acari: Ixodidae). Journal of Medical Entomology 45, 817-831. Avarzed, A., Igarashi, I., Kanemaru, T., Hirumi, K., Omata, Y., Saito, A., Oyamada, T., Nagasawa, H., Toyoda, Y., Suzuki, N., 1997. Improved in vitro cultivation of Babesia caballi. J Vet Med Sci 59, 479-481. Bahrami, S., Ghadrdan, A.R., Mirabdollahi, S.M., Fayed, M.R., 2014a. Diagnosis of subclinical equine theileriosis in center of Iran using parasitological and molecular methods. Trop Biomed 31, 110-117. Bahrami, S., Ghadrdan, A.R., Pourmahdi, M., Salarpour, M.V., 2014b. Epidemiology of Theileria equi in Persian Arab horses from Iran. Veterinarni Medicina 59, 409-414. Beck, R., Vojta, L., Mrljak, V., Marinculic, A., Beck, A., Zivicnjak, T., Caccio, S.M., 2009. Diversity of Babesia and Theileria species in symptomatic and asymptomatic dogs in Croatia. Int J Parasitol 39, 843-848. Boid, R., Jones, T.W., Luckins, A.G., 1985. 3. Protozoal diseases of camels. British Veterinary Journal 141, 87-105. Bukachi, S.A., Chemuliti, J.K., Njiru, Z.K., 2003. Constrains experienced in the introduction of camels in tsetse fly infested areas: the case of Kajiado District, Kenya. Journal of Camel Practice and Research 10 145–148. Criado-Fornelio, A., Martinez-Marcos, A., Buling-Saraña, A., Barba-Carretero, J.C., 2003. Molecular studies on Babesia, Theileria and Hepatozoon in southern Europe: Part I. Epizootiological aspects. Veterinary Parasitology 113, 189-201. de Waal, D.T., 1992. Equine piroplasmosis: A review. British Veterinary Journal 148, 6-14. Egbe-Nwiyi, T.N., 1994. Haematological and pathological studies of camel babesiosis in Nigeria. Bulletin of Animal Health and Production in Africa 42, 287–290. El-Rabie, Y., Amr, Z.S., Hyland, K.E., 1990. Observations on the ixodid ticks associated with domestic ungulate animals in Jordan. Zoology in the Middle East 4, 85-92. Fritz, D., 2010. A PCR study of piroplasms in 166 dogs and 111 horses in France (March 2006 to March 2008). Parasitol Res 106, 1339-1342. Garcia-Bocanegra, I., Arenas-Montes, A., Hernandez, E., Adaszek, L., Carbonero, A., Almeria, S., Jaen-Tellez, J.A., Gutierrez-Palomino, P., Arenas, A., 2013. Seroprevalence and risk factors associated with Babesia caballi and Theileria equi infection in equids. Vet J 195, 172-178. Heim, A., Passos, L.M., Ribeiro, M.F., Costa-Junior, L.M., Bastos, C.V., Cabral, D.D., Hirzmann, J., Pfister, K., 2007. Detection and molecular characterization of Babesia caballi and Theileria equi isolates from endemic areas of Brazil. Parasitol Res 102, 63-68. Karatepe, B., Karatepe, M., Cakmak, A., Karaer, Z., Ergun, G., 2009. Investigation of seroprevalence of Theileria equi and Babesia caballi in horses in Nigde province, Turkey. Trop Anim Health Prod 41, 109-113. Kouam, M.K., Kantzoura, V., Gajadhar, A.A., Theis, J.H., Papadopoulos, E., Theodoropoulos, G., 2010. Seroprevalence of equine piroplasms and host-related factors associated with infection in Greece. Vet Parasitol 169, 273-278. Leemans, I., Brown, D., Fossum, C., Hooshmand-Rad, P., Kirvar, E., Wilkie, G., Uggla, A., 1999. Infectivity and cross-immunity studies of Theileria lestoquardi and Theileria annulata in sheep and cattle: II. In vitro studies. Veterinary Parasitology 82, 193-204. Moretti, A., Mangili, V., Salvatori, R., Maresca, C., Scoccia, E., Torina, A., Moretta, I., Gabrielli, S., Tampieri, M.P., Pietrobelli, M., 2010. Prevalence and diagnosis of Babesia and Theileria infections in horses in Italy: a preliminary study. Vet J 184, 346-350. Mujica, F.F., Perrone, T., Forlano, M., Coronado, A., Melendez, R.D., Barrios, N., Alvarez, R., Granda, F., 2011. Serological prevalence of Babesia caballi and Theileria equi in horses of Lara State, Venezuela. Vet Parasitol 178, 180-183. Posnett, E.S., Ambrosio, R.E., 1991. DNA probes for the detection of Babesia caballi. Parasitology 102, 357-365. Posnett, E.S., Fehrsen, J., De Waal, D.T., Ambrosio, R.E., 1991. Detection of Babesia equi in infected horses and carrier animals using a DNA probe. Veterinary Parasitology 39, 19-32. Qablan, M.A., Kubelova, M., Siroky, P., Modry, D., Amr, Z.S., 2012a. Stray dogs of northern Jordan as reservoirs of ticks and tick-borne hemopathogens. Parasitol Res 111, 301-307. Qablan, M.A., Sloboda, M., Jirku, M., Obornik, M., Dwairi, S., Amr, Z.S., Horin, P., Lukes, J., Modry, D., 2012b. Quest for the piroplasms in camels: identification of Theileria equi and Babesia caballi in Jordanian dromedaries by PCR. Vet Parasitol 186, 456-460. Rao, J.R., Mishra, A.K., Sharma, N.N., Kalicharan Prasad, M.C., 1988. Biochemical studies on sera of camels (Camelus dromedarius) naturally infected with Theileria dromedarii n. sp. Riv. Parasitology 49, 63–66. Ruegg, S.R., Torgerson, P., Deplazes, P., Mathis, A., 2007. Age-dependent dynamics of Theileria equi and Babesia caballi infections in southwest Mongolia based on IFAT and/or PCR prevalence data from domestic horses and ticks. Parasitology 134, 939-947. Salim, B.O., Hassan, S.M., Bakheit, M.A., Alhassan, A., Igarashi, I., Karanis, P., Abdelrahman, M.B., 2008. Diagnosis of Babesia caballi and Theileria equi infections in horses in Sudan using ELISA and PCR. Parasitol Res 103, 1145-1150. Sevinc, F., Maden, M., Kumas, C., Sevinc, M., Ekici, O.D., 2008. A comparative study on the prevalence of Theileria equi and Babesia caballi infections in horse sub-populations in Turkey. Vet Parasitol 156, 173-177. Sigg, L., Gerber, V., Gottstein, B., Doherr, M.G., Frey, C.F., 2010. Seroprevalence of Babesia caballi and Theileria equi in the Swiss horse population. Parasitol Int 59, 313-317. Uilenberg, G., 2006. Babesia--a historical overview. Vet Parasitol 138, 3-10. Walker, J.B., Keirans, J.E., Horak, I.G., 2005. The Genus Rhipicephalus (Acari, Ixodidae): A Guide to the Brown Ticks of the World, Cambridge University Press. Yakimoff, W.L., Schokhor, N.I., Kosel-Kine, P.M., 1917. In Maladies animals du Turkestan russe a parasites endoglobulaires. Bulletin de la Societe de Pathologie Exotique 10, 303–309. | ||
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