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Effects of experimental Mesobuthus eupeus scorpion envenomation on chicken | ||
| Archives of Razi Institute | ||
| مقاله 4، دوره 72، شماره 1، خرداد 2017، صفحه 23-31 اصل مقاله (2.61 M) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22034/ari.2016.107493 | ||
| نویسندگان | ||
| Mohammad Khosravi* 1؛ Mansour Mayahi2؛ Seyedeh Missagh Jalali2؛ Anahita Rezaie1؛ Ahmad Taghavi Moghadam3؛ Zohreh Hosseini4؛ Seyedeh Kolsum Barzegar4؛ Somayeh Azadmanesh4 | ||
| 1Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Khuzestan, Iran | ||
| 2Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Khuzestan, Iran | ||
| 3Razi Reference Laboratory of Scorpion Research, Razi Vaccine and Serum Research Institute, Ahvaz, Iran | ||
| 4Student of Veterinary Medicine, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran. | ||
| چکیده | ||
| This study aimed to evaluate the clinical, histopathological and hematological effects of Mesobuthus eupeus venom on chicken organs. Adult chickens were subcutaneously injected with five doses of M. eupeus venom (0.5, 2, 5, 10 and 20 mg/kg; four chickens per each dose). Symptoms were recorded during the experiment and blood samples were collected for hematological analysis. Moreover, a complete necropsy was performed. After macroscopic examination, tissue samples were obtained from the liver, kidneys, heart, lungs, intestines and brain of the chickens three days after venom administration. In intravenous injection, lethal dose of the venom was determined at 15 mg/kg. The first clinical, pathological and hematological symptoms in envenomated chickens were observed at M. eupeus doses of 2, 5 and 0.5 mg/kg, respectively. Hematological examination revealed a reduction in lymphocyte count following experimental envenomation, which returned to the pre-experiment level in almost all the cases. On the other hand, heterophil count was found to increase during the experimental period. In addition, erythrocyte count and hematocrit level were stable at all the intervals. Pathological examination was indicative of severe pulmonary hemorrhage, pulmonary and cerebral edema, tubular necrosis of the kidneys, hemorrhage in kidneys and heart, hyaline thrombus and congestion of the liver. According to the results of this study, poultry are resistant to the toxic effects of M. eupeus venom. | ||
| کلیدواژهها | ||
| Mesobuthus eupeus؛ Venom؛ Chicken؛ Pathology؛ Hematology | ||
| عنوان مقاله [English] | ||
| اثر گزش تجربی عقرب مزوبتوس اپئوس در ماکیان | ||
| نویسندگان [English] | ||
| محمد خسروی1؛ منصور میاحی2؛ سیده میثاق جلالی2؛ آناهیتا رضایی1؛ احمد تقوی مقدم3؛ زهره حسینی4؛ سمیه آزادمنش4؛ | ||
| 1گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران | ||
| 2گروه علوم بالینی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران | ||
| 3شعبه جنوب غرب موسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| 4دانشجو، دانشکده دامپزشکی، دانشگاه شهید چمران، اهواز، ایران | ||
| چکیده [English] | ||
| این مطالعه اثرات بالینی، هماتولوژی و پاتولوژی زهر عقرب مزوبتوس اپئوس در ماکیان را بررسی نموده است. پنج دوز از زهر مزوبتوس اپئوس (5/0، 2، 5، 10 و 20 mg/kg) به صورت زیر پوستی به ماکیان بالغ، شامل چهار قطعه در هر گروه تزریق شد. در زمان مطالعه علائم بالینی ثبت شد: نمونه خون جهت بررسی هماتولوژی اخذ شد و کالبد گشایی کامل انجام شد. سه روز پس تزریق سم، ارزیابی ظاهری انجام شد و نمونه های بافتی از کبد، کلیه، قلب، ریه، روده و مغز اخذ شد. میزان کشندگی در تجویز داخل وریدی 15 mg/kg بود. نخستین علائم بالینی، پاتولوژی و هماتولوژی به ترتیب در مقادیر 2، 5 و 5/0 mg/kg مشاهده شد. ارزیابی هماتولوژی پس از گزش تجربی بیانگر کاهش میزان لنفوسیتها پس از گزش تجربی و تقریبا بازگشت به میزان طبیعی در همه گروهها بود، درحالیکه میزان هتروفیلها طی زمان آزمایش افزایش یافته بود. علاوه بر این تعداد گلبولهای سفید و هماتوکریت در همه فواصل نمونه گیری ثابت بود. بررسی پاتولوژیک نمایانگر خونریزی شدید ریوی، ادم ریوی و مغزی، نکروز لوله های کلیوی، خونریزی از کلیه و قلب، ترومبوز هیالینی و احتقان کبد بود. با توجه نتایج ماکیان تقریبا نسبت به اثرات سمی زهر مقاوم می باشند. | ||
| کلیدواژهها [English] | ||
| مزوبتوس اپئوس, زهر, ماکیان, هماتولوژی, پاتولوژی | ||
| مراجع | ||
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