| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 2,998 |
| تعداد مقالات | 33,579 |
| تعداد مشاهده مقاله | 44,250,976 |
| تعداد دریافت فایل اصل مقاله | 20,621,949 |
Induction of Callus and Somatic Embryogenesis from Cotyledon Explants of Fagonia indica Burm. | ||
| Journal of Medicinal plants and By-products | ||
| مقاله 14، دوره 2، شماره 2، آذر 2013، صفحه 209-214 اصل مقاله (154.28 K) | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2013.108596 | ||
| نویسندگان | ||
| Mohammad Ali Ebrahimi* ؛ Aida Payan | ||
| Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran. | ||
| چکیده | ||
| To explore the potential for in vitro rapid regeneration of Fagonia indica Burm., cotyledon explants were obtained from 10-day old aseptically germinated seedlings that cultured on MS medium which supplemented with 1.0-5.0 µM 2,4-D. Callus was initiated from cotyledon explant on MS medium supplemented in all concentrations of 2,4-D investigated. 2,4-D was more effective than IAA and NAA for callus induction, but auxins and cytokines’ combination is essential for the green callus induction for somatic embryogenesis. Induced calluses were subcultured on media containing 2.5-5.0 µM BA or Kin combined with different concentrations IAA, NAA and 2,4-D. Elimination of 2,4-D and a slight increase of BA concentration induced of the relating somatic embryogenesis. The maximum globular structure embryos were further enlarged and produced the somatic embryos in MS basal medium supplemented with 5.0 BA µM and also combined with either 2.5 µM NAA. Continued formation of the globular embryo occurred in these mediums for 12 months. The maximum germinations of embryos were observed in MS medium + 1.5% sucrose without growth regulators. | ||
| کلیدواژه‌ها | ||
| Fagonia indica Burm. f؛ Somatic Embryogenesis؛ Callus | ||
| مراجع | ||
|
1. Naglaa GS, Amina M, Saeed AK, Salma MN. Chemical constituents and biological activities of Fagonia indica Burm F. Res J Med plant. 2011;1819-3455.
2. Rahman I, Bel A, Mulier B, Lawson MF, Harrison DJ, MacNee W, Smith CAD. Transcriptional regulation of glutamylcysteine synthetase-heavy subunit by oxidants in human alveolar epithelial cells. BiochemBiophys Res Commun. 1996;229:832-837.
3. Eman A. Alam. Phytochemical Screening on Calli of Fagonia indica and Fagonia bruguieri Dc. New York Sci J. 2010;3:158-164.
4. Singh MP, Dey S, Singh BS. Conservation of Biodiversity and Natural Resources. Daya Publishing House, Delhi, 2004 pp. 12-45.
5. Kayser O, Quax W. Medicinal Plant Biotechnology, from basic research to industrial application. Willy-VCH GmbH & Co.KGaA publisher, 2007;187-199.
6. Eman A. Alam, Gehan HA, Yassin ME, Mohamed SA. Chemical composition and antibacterial activity studies on callus of Fagonia arabica L. Academia Arena. 2010;2:91-106.
7. Duncan DB. Multiple range and multiple F-tests. Biometrics 1955;11:1-42.
8. Gamborg OL, Phillips GC. In: Plant Cell, Tissue and Organ Culture Fundamental methods (ed). Narosa, Publishing House, New Delhi, 1998, pp 87-95.
9. Warren G. The regeneration of plants from cultured cells and tissues. In: Plant Cell and Tissue Culture. A. Stafford and G. Warren (Eds.), 1991, p 85.
10. Oluwaseun AS, Erhinmeyoma AB. Induction of callus and somatic embryogenesis from cotyledon explants of Parkia biglobosa (Jacq.) Benth. African J of Biotech. 2005;4:68-71.
11. Dode LC, Bobrowski VL, Braga EJB, Seixas FK, Schunch W. In vitro propagation of Ocimum basilicum L. Maringa. 2003;25:435-437.
12. Yasmin R, Javed F, Arfan M. Somatic embryogenesis in callus culture of wheat Triticum aestivum L. Int. J Agric Bio. 2001;3:163-166.
13. Sasse F, Heckenberg U, Berlin J. Accumulation of β-Carboline Alkaloids and serotonin by cell cultures of Peganum harmala L. Plant Physiol. 1982;69:400-404.
14. Kim SW, Dong SI, Pil SC, Jang RL. Plant regeneration from immature zygotic embryo derived embryogenic calluses and cell suspension cultures of Catharanthus roseus. Plant Cell Tiss Org Cul. 2004;76:131-135.
15. Ammirato PV. Growth and morphogenesis in cultures of the monocot yam, Dioscorea. In: Fujiwara A (ed.) Plant Tissue Culture. Maruzen, Tokyo, 1982, pp 169-170.
16. Hiraoka N, Kodama T. Effects of non frozen cold storage on the growth organogenesis and secondary metabolism of callus cultures. Plant Cell Tiss. Org Cul 1984;3:349-357.
17. Nikam TD, Ebrahimi MA, Patil VA. Embryogenic callus culture of Tribulus terrestris L. a potential source of harmaline, harmine and diosgenin. Plant Biotechnol. Rep. 2009;3:243-250.
18. Godbole S, Sood A, Thakur R, Sharma M, Ahuja PS. Somatic embryogenesis and its conversion into plantlets in a multipurpose bamboo, Dendrocalamau hamiltonii Nees et Arn. Ex Munro Curr Sci. 2002;83:885-889.
19. Obembe OO, Adebona AC, Esan EB. Effect of plant growth regulators on callus growth of Cola nitida (Malvales: Sterculiaceae). Biosci Res Comm. 1999;11:53-57.
20. Ehsanpour AA. Induction of somatic embryogenesis from endosperm of Oak (Quercus castanifolia). Australian J Biology. 2002;273-277.
21. Lee Ek, Cho DY, Soh WY. Effect of humidity on somatic embryogenesis in cotyledon explant culture of carrot J plant Biol.1997;40:89-94.
22. Zimmerman JL. Somatic embryogenesis: A model for early development in higher plants. Plant Cell 1993;5:1411-1423. | ||
|
آمار تعداد مشاهده مقاله: 450 تعداد دریافت فایل اصل مقاله: 570 |
||