| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شمارهها | 3,092 |
| تعداد مقالات | 34,514 |
| تعداد مشاهده مقاله | 49,344,365 |
| تعداد دریافت فایل اصل مقاله | 79,806,540 |
A Study of Genetic and Chemical Diversities of some Chamomile Ecotypes Based on RAPD Markers and Essential oil Compositions | ||
| Journal of Medicinal plants and By-products | ||
| مقاله 6، دوره 3، شماره 1، تیر 2014، صفحه 41-46 اصل مقاله (211.26 K) | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2014.108602 | ||
| نویسندگان | ||
| Amin Baghizadeh* 1؛ Adele Balouchi2؛ Habibolah Nazem2 | ||
| 1Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran | ||
| 2Payame Noor University of Tehran, Tehran, Iran | ||
| چکیده | ||
| Chamomile is a medicinal plant with high economic value. In this research, 20 chamomile ecotypes collected from different regions of Iran were evaluated for genetic and chemical diversity. DNA was extracted by CTAB and polymerase chain reaction was performed using 13 RAPD markers. Essential oils extraction was performed by water distillation using Clevenger system. Components of the essential oils were detected by Gas Chromatography at 62 retention times. The results showed matching relation between chemical composition and geographical diversity. It is important to note that in this study, Pseudo chamomile ecotypes were placed next to German chamomile ecotypes in both molecular and chemical analyses. In order to identify essential oil compositions, the essential oils of 5 ecotypes were analysed using GC/MS method. The major components in the oil were, α-bisabolon oxide A, α-bisabolol oxide A , and (Z )- β-Farnesene. Regarding percentage of three important medicinal compositions of chamomile, Esphandegheh ecotype was chosen as the best one. | ||
| کلیدواژهها | ||
| Matricaria chamomilla؛ essential oil composition؛ Genetic diversity؛ GC/MS | ||
| مراجع | ||
|
1. Omidbeigi R. Study on chemotypes of Iranian wild grown chamomile and its comparison with improved types. J Modares Agrric Sci. 1999;1:45-53.
2. Shipochliev T, Dimitrov A, Aleksandrova E. Anti-inflammatory action of a group of plant extracts. Vet Med Nauki. 1981;18:87-94.
3. Gupta V, Mittal p, Bansal p, Khokra S, Kaushik D. Pharmacological Potential of Matricaria recutita-A Review. International Journal of Pharmaceutical Sciences and Drug Research. 2010;2:12-16.
4. Uteshev B, Laskova I, Afanasev V. The immunomodulating activity of the heteropolysaccharides from German chamomile (Matricaria chamomilla) during air and immersion cooling, EkspKlin Farmakol. 1999;62:52-59.
5. Mann C, Staba E. The Chemistry, Pharmacology and Commercial formulations of Chamomile. J Herb Spice Med Plants. 1986;1:235-280.
6. Williams J, Kubeik A, Livak K, Rafalski J,Tingey S. DNA polymorphism Amplified by arbitrary primers are useful as genetic marker. Nucleic Acid Res. 1990;18:6531-6535.
7. Taviana P. Variation for agronomic and essential oil traits among wild populations of Chamomilla recutita L. Rausch from Central Italy. J Herb Spice Med Plants. 2001;9:1049-6475.
8. D' Andrea L. Variation of morphology, yield and essential oil components in common chamomile (Chamomilla recutita L.) Rauschert cultivars Grown in Southern Italy. J Herb Spice Med Plants. 2002;9:359-365.
9. Wagner C, Friedt W, Marquard R, Ordon F. Molecular analyses on the genetic diversity and inheritance of (-)-a- bisabolol and chamazulene content in tetraploid chamomile (Chamomilla recutita L.) Rausch. Plant Sci. 2005;169:917-927.
10. Jaimand K, Rezaei MB. Analysis of essence components of chamomile (M. chamomilla) in Tehran, Hamedan and Kazeroun. Iran. J Med Arom Plants. 2002;305:11-15.
11. Solouki M, Mehdikhani H, Zeinali H, Emamjomeh A. Study of genetic diversity in Chamomile (Matricaria chamomilla) based on morphological traits and molecular markers. Sci Hort. 2008;117:281-287.
12. Pirkhezri M, Hassani M, Hadian J. Genetic diversity in different populations of Matrica riachamomilla L. Growing in southwest of Iran based on morphological and RAPD markers.Research. J Med Plants. 2010;4:1-13.
13. Nazaralipour A,Sefidkon F.Quantitative and Qualitative study of the Essential oil from aromatic and medicinal Tripleurospermum disciforme.J Med plants. 2003;2:33-40.
14. Ghanavati M, Houshmand H, Zainali F, Ejlali F. Salinity effects on germination and growth of chamomile genotypes. J Med Plants Res. 2011;5:6609-6614.
15. Gichuki S, Berenyi M, zhang D, Hermann M, Schmidt J, glossol J, Burg K. genetic diversity in sweet potato (Ipomeaatatas) in relationship to geographic source as assessed with RAPD markers, genet crop ev.2003;5:429-37.
16. Salamon I. Production of chamomile, Chamomilla recutita (L.) Rauschert, in Slovakia. J Herb Spice Med Plants. 1992;1:37-45.
17. Stingeni L, Agea E, Lisi P, Spinozzi F. T-lymphocyte cytokine profiles in Compositaeairborne dermatitis. British J Derma. 1999;141:689-693. | ||
|
آمار تعداد مشاهده مقاله: 607 تعداد دریافت فایل اصل مقاله: 734 |
||