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Hassanloo, Thahereh, Jafarkhani Kermani, Maryam, Malmir Chegini, Mahsa, Sepehrifar, Roshanak, Mohajeri Naraghi, Sepehr, Miri, Seied Mehdy. (1393). Optimization of In vitro Propagation of Qare-Qat (Vaccinium arctostaphylus). سامانه مدیریت نشریات علمی, 3(2), 199-205. doi: 10.22092/jmpb.2014.108734
Thahereh Hassanloo; Maryam Jafarkhani Kermani; Mahsa Malmir Chegini; Roshanak Sepehrifar; Sepehr Mohajeri Naraghi; Seied Mehdy Miri. "Optimization of In vitro Propagation of Qare-Qat (Vaccinium arctostaphylus)". سامانه مدیریت نشریات علمی, 3, 2, 1393, 199-205. doi: 10.22092/jmpb.2014.108734
Hassanloo, Thahereh, Jafarkhani Kermani, Maryam, Malmir Chegini, Mahsa, Sepehrifar, Roshanak, Mohajeri Naraghi, Sepehr, Miri, Seied Mehdy. (1393). 'Optimization of In vitro Propagation of Qare-Qat (Vaccinium arctostaphylus)', سامانه مدیریت نشریات علمی, 3(2), pp. 199-205. doi: 10.22092/jmpb.2014.108734
Hassanloo, Thahereh, Jafarkhani Kermani, Maryam, Malmir Chegini, Mahsa, Sepehrifar, Roshanak, Mohajeri Naraghi, Sepehr, Miri, Seied Mehdy. Optimization of In vitro Propagation of Qare-Qat (Vaccinium arctostaphylus). سامانه مدیریت نشریات علمی, 1393; 3(2): 199-205. doi: 10.22092/jmpb.2014.108734

Optimization of In vitro Propagation of Qare-Qat (Vaccinium arctostaphylus)

Journal of Medicinal plants and By-product
مقاله 15، دوره 3، شماره 2، آذر 2014، صفحه 199-205    اصل مقاله (1.59 M)
شناسه دیجیتال (DOI): 10.22092/jmpb.2014.108734
نویسندگان
Thahereh Hassanloo* 1؛ Maryam Jafarkhani Kermani2؛ Mahsa Malmir Chegini3؛ Roshanak Sepehrifar1؛ Sepehr Mohajeri Naraghi1؛ Seied Mehdy Miri3
1Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran (ABRII), P.O. Box 31535-1897, Karaj, Iran
2Department of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran
3Department of Horticulture, Agriculture and Natural Resources Faculty, Islamic Azad University-Karaj Branch, Karaj, Iran
چکیده
In order to optimize the micropropagation of Qare-Qat (Vaccinium arctostaphylus J.J.Sm), samples were collected from two regions of Iran (Asalem and Kelardasht). Anderson (AN) media containing different concentrations of zeatin (0, 0.5, 1, 2 and 4 mg L-1) and different levels of pH (4.5, 5 and 5.5) were compared. The in vitro shoots were transferred on AN media supplemented with zeatin (1 and 2 mg L-1) alone or in combination with Indole-3-butyric acid (IBA) (0.2 and 0.4 mg L-1). To optimize the rooting stage, elongated shoots (1.5- 2cm) were cultured on half strength semi- solid media containing activated charcoal (0 and 7 g L-1),or half strength liquid AN medium supplemented with different concentrations of IBA (0, 0.5, 2 and 3 mg L-1) and NAA (0 and 0.5 mg L-1). The results indicated that at the establishment stage AN media supplemented with 4 mg L-1 zeatin (pH= 5.5) and 1 mg L-1 zeatin (pH= 5) were the best treatment for Asalem and Kelardasht explants, respectively. For the Asalem explants, the highest number of proliferated shoots (4) was observed on AN media consisting of 2 mg L-1 zeatin and 0.4 mg L-1 IBA. Whereas for the Kelardash explants  the highest number of proliferated shoots (3) was observed on AN media consisting of 2 mg L-1 zeatin and 0.2 mg L-1 IBA. The highest percent of root formation (86 and 66%) were observed in half strength AN medium containing of 7 g L-1 activated charcoal and 3 mg L-1 IBA and 0.5 mg L-1 NAA in Asalem and Kelardasht explants, respectively. The plantlets were successfully transferred to soil and the survival rate was 85%.
کلیدواژه‌ها
Medicinal plants؛ Micropropagation؛ in vitro؛ growth regulators؛ Vaccinium arctostaphylus
مراجع
1. Hasanloo T, Sepehrifar R, Hajimehdipoor H. Levels of phenolic compounds and their effects on antioxidant capacity of wild Vaccinium arctostaphylus L. (Qare- Qat) collected from different regions of Iran. Turk J Biol.2011; 35: 371-377.

2. Sedaghathoor SH, Kashi AK, Talae AR, Khalighi A. Essential oils of Qare-Qat (Vaccinium arctostaphylus) shoots and chemical composition of berries. Int J Agr Biol. 2006;1:45-46.

3. Meiners J, Schwab M, Szankowski I. Efficient in vitro regeneration systems for Vaccinium species. Plant Cell Tiss Org Cult. 2007;89:169-176.

4. Marcotrigiano M, McGlew SP. A two-stage micropropagation system for cranberries. J Am Soc Hortic Sci. 1991;116:911-916.

5. Debnath SC, McRae KB. A one-step in vitro cloning procedure for cranberry (Vaccinium acrocarpon Ait.): the influence of cytokinins on shoot proliferations and rooting. Small Fruits Rev. 2005;4:57-75.

6. Reed BM, Abdelnour-Esquivel A. The use of zeatin to initiate in vitro cultures of Vaccinium species and cultivars. Hort Sci. 1991; 26: 1320-1322.

7. Eccher T, Noe N. Comparison between 2iP and zeatin in the micropropagation of highbush blueberry (Vaccinium corymbosum). ISHS Acta Hortic. 1989;241:185-190.

8. Morrison S, Smagula JM. Morphology, growth, and rhizome development of Vaccinium angustifolium Ait. seedlings, rooted softwood cuttings, and micropropagated planted. Hort Sci. 2000;35:738-741.

9. Mohajeri Naraghi S, Mardi M, Hasanloo T, Pirseyedi SM, Mahmoodi P. Isolation and characterization of novel microsatellite loci in Vaccinium arctostaphylus L. Conservation Genetics Resources. 2011;3:441- 444.

10. Ostrolucká MG, Gadšová A, Ondrušková E, Libiaková G, Lateekov M. Effect of medium pH on axillary shoot proliferation of selected Vaccinium vitis-idaea cultivars. Acta Biologica Cracoviensia Series Botanica. 2010;52:92-96.

11. Borkowska B. Wymagania roślin borَwki wysokiej pochodzacych z in vitro. Ogrodnictwo. 1996;2: 17– 18.

12. Debnath SC. Micropropagation of small fruits. In: Jain SM, and Ishii K [eds.], Micropropagation of Woody Trees and Fruits. Kluwer Academic Publishers Dordrecht. 2003;456-506.

13. Debnath SC. In vitro culture of lowbush blueberry (Vaccinium angustifoliumait). Small Fruits. Rev. 2004;3:393- 408.

14. Debnath SC. Effects of carbon source and concentration on development of lingonberry (Vaccinium vitis-idaea L.) shoots cultivated in vitro from nodal explants. In vitro Cellular and Developmental Biol. 2005;41:145-150.

15. Jaakola L, Tolvanen A, Laine K, Hohtola A. Effect of N6-isopentenyladenine concentration on growth initiation in vitro and rooting of bilberry and lingonberry microshoots. Plant Cell Tiss Org Cult. 2001;66:73-77.

16. Qu L, Polashock J, Vorsa N. A highly efficient in vitro cranberry regeneration system using leaf explants. Hort Sci. 2000; 35:948-952.

17. Ružić D, Vujović T, Libiakova G, Cerović R, Gajdošova A. Micropropagation in vitro of high bush bluebeery (Vaccinium corymbosum). Iss J berry research. 2012; 2: 97- 103.

18. Debnath SC, McRae KB. In vitro culture of lingonberry (Vaccinium vitis-idaea L.): The influence of cytokinins and media types on propagation. Small Fruits Rev. 2001;1:3-19.

19. Phulwaria M, Ram K, Harish, GuptaAK, Shekhawat NS. Micropropagation of mature Terminalia catappa (Indian Almond), a medicinally important forest tree. J Forest Res. 2012;17:202-207.

20. Ostrolucká MG, Libiaková G, Ondrusková A, Gajdosová A. In vitro propagation of Vaccinium species. Acta Univ Latv. 2004;676:207-212.

21.Ostrolucká MG, Gajdošová A, Ondrušková E,Libiaková G. In vitro propagation of several Vaccinium corymbosum L. and Vaccinium vitis-idaea L. cultivars. Issue of the International Scientific Conference and NJF seminar. 2009; 12: 75-80.

22. Guang-jie Z, Zhan-bin W, Dan W. In vitro propagation and ex vitro rooting of blueberry plantlets. Plant Tiss Cult and Biotech. 2008;18:187-195.

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