Mohammadfam, M., Golkari, S., Sadeghzadeh, B. (2016). Molecular characterization of allelic variation at the VRN-1 and VRN-B3 loci controlling vernalization requirement in wheat genotypes based on polymorphism in the promoter and intron regions. , 5(2), 185-201. doi: 10.22092/idaj.2016.109665
M. Mohammadfam; Saber Golkari; Behzad Sadeghzadeh. "Molecular characterization of allelic variation at the VRN-1 and VRN-B3 loci controlling vernalization requirement in wheat genotypes based on polymorphism in the promoter and intron regions". , 5, 2, 2016, 185-201. doi: 10.22092/idaj.2016.109665
Mohammadfam, M., Golkari, S., Sadeghzadeh, B. (2016). 'Molecular characterization of allelic variation at the VRN-1 and VRN-B3 loci controlling vernalization requirement in wheat genotypes based on polymorphism in the promoter and intron regions', , 5(2), pp. 185-201. doi: 10.22092/idaj.2016.109665
Mohammadfam, M., Golkari, S., Sadeghzadeh, B. Molecular characterization of allelic variation at the VRN-1 and VRN-B3 loci controlling vernalization requirement in wheat genotypes based on polymorphism in the promoter and intron regions. , 2016; 5(2): 185-201. doi: 10.22092/idaj.2016.109665

Molecular characterization of allelic variation at the VRN-1 and VRN-B3 loci controlling vernalization requirement in wheat genotypes based on polymorphism in the promoter and intron regions

زراعت دیم ایران
Article 5, Volume 5, Issue 2 - Serial Number 10, January 2017, Pages 185-201    PDF (842.8 K)
Document Type: Research Paper
DOI: 10.22092/idaj.2016.109665
Authors
M. Mohammadfam1; Saber Golkari* 2; Behzad Sadeghzadeh2
1Department of Biotechnology,Maragheh branch, Islamic Azad University, Matagheh, Iran
2Dryland Agricultural Research Institute, Agricultural Research Education and Extension Organization (AREEO), Maragheh, Iran
Abstract
Wheat, as a major crop in Iran and in the world, is considered an important crop for human consumption. Identification of allelic variations at loci that control vernalization requirements and cold tolerance, provides valuable information for determining adaptability of wheat genotypes to target environments. At molecular level, vernalization in wheat (Triticum aestivum L.) is determined mainly by three VRN-1, VRN-2 and VRN-3 loci. In hexaploid wheat, dominant alleles Vrn-A1, Vrn-B1 and Vrn-D1 are responsible for spring growth habit and recessive allels with epistatic interactions determine the winter growth habit. In this study, allelic variations among 50 wheat genotypes were studied using 9 primer pairs specific to VRN-1 and VRN-B3 loci. Dominant alleles at Vrn-B1,Vrn-D1 and Vrn-B3 loci were detected with the frequency of %58, %24 and %90, respectively. Recessive alleles vrn-B1, vrn-D1 and vrn-B3 had a frequency of %42, %76 and %10, respectively. At Vrn-A1 locus, different alleles were detected with the frequency of Vrn-A1c (58%), Vrn-A1b (26%), Vrn-A1a (14%) and vrn-A1 (2%). Our finding revealed a considerable level of variations at gene loci controlling vernalization and cold tolerance in dryland wheat genotypes. Data provided here may be used for producing new wheat cultivars adapted to the target environments.
Keywords
Wheat; Vernalization; spring growth habit; winter growth habit; VRN-1 and VRN-B3
References
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