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اثرات القاء پلی‌پلوئیدی در شرایط درون شیشه‌ای بر برخی خصوصیات مورفولوژیکی، فیزیولوژیکی و بیوشیمیایی گیاه نوروزک ((Salvia leriifolia Benth. | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 3، دوره 25، شماره 1 - شماره پیاپی 49، خرداد 1396، صفحه 24-42 اصل مقاله (510.92 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2017.109815 | ||
| نویسندگان | ||
| بهمن حسینی* 1؛ سپیده جوانبخت2 | ||
| 1هیات علمی دانشگاه ارومیه | ||
| 2کارشناس ارشد، علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه | ||
| چکیده | ||
| نوروزک (.Salvia leriifolia Benth) گیاهی علفی و چندساله، متعلق به تیره نعناعیان که در سال‌های اخیر خواص مختلف دارویی این گیاه شناخته شده است. پلی‌پلوئیدی درون شیشه‌ای گیاهان دارویی در سال‌های اخیر یکی از موضوعات جالب توجه در بیوتکنولوژی و کشت بافت گیاهی می‌باشند. بر این اساس، با استفاده از غلظت‌های مختلف کلشیسین (0، 05/0، 1/0، 2/0 و 5/0 درصد) در سه سطح زمانی 24، 48 و 72 ساعت، القای پلی‌پلوئیدی در جوانههای انتهایی گیاهچههای باززا شده نوروزکمطالعه شد. برای شناسایی گیاهان پلیپلوئید، خصوصیات مورفولوژیکی، فیزیولوژیکی، بیوشیمایی و سیتوژنتیکی بررسی شد. نتایج نشان داد کهتأثیر تیمارهای مختلف کلشیسین بر درصد زندهمانی گیاهچههای حاصل در سطح احتمال 1 درصد معنیدار میباشد. با مقایسه تیمارهای 05/0، 1/0، 2/0 و 5/0 درصد کلشیسین، بالاترین میزان زندهمانی ریزنمونهها در تیمار 05/0 درصد برابر با 44/84 درصد مشاهده شد. همچنین بالاترین میزان مرگ گیاهچه، در غلظت 5/0 درصد کلشیسین ثبت شد. القاء تتراپلوئیدی سبب تغییرات مورفولوژیکی، فیزیولوژیکی، سیتولوژیکی و ترکیبات شیمیایی از قبیل افزایش طول و عرض روزنه در سطح زیرین برگ، عرض برگ، سطح برگ، میزان کلروفیل برگ، میزان آنتیاکسیدان کل، فنل کل و فعالیت آنزیمهای آنتیاکسیدانی شد (آنزیمهای کاتالاز و آسکوربات پراکسیداز)، همچنین موجب کاهش طول برگ و تراکم روزنه در واحد سطح برگ شد. تعداد کروموزوم گیاهان دیپلوئید برابر با 22 عدد (2n=2x=22) و در گیاهان تتراپلوئید برابر با 44 (2n=4x=44) بود. بنابراین می‌توان بیان کرد که کلشیسین بهطور مؤثری قابلیت القای پلیپلوئید در گیاه نوروزک را دارد. | ||
| کلیدواژه‌ها | ||
| نوروزک؛ کلشیسین؛ پلی‌پلوئیدی؛ کشت درون شیشه | ||
| عنوان مقاله [English] | ||
| Effects of in vitro polyploidy induction on some morphological, physiological and biochemical traits of Salvia leriifolia Benth | ||
| نویسندگان [English] | ||
| B. Hosseini1؛ S. Javanbakht2 | ||
| 1Assoc. Prof., Horticulture Department, Urmia University, Urmia, I.R. Iran | ||
| 2M.Sc., Horticulture Department, Urmia University, Urmia, I.R. Iran | ||
| چکیده [English] | ||
| Salvia leriifolia Bent. is a perennial herbaceous plant belongs to Lamiaceae family. Different pharmacological activities of the species were evaluated during recent years. Medicinal plant In vitro ploidy induction was one of the interesting issues in biotechnology and plant tissue culture. Therefore, influence of different colchicineconcentrations (0, 0.05, 0.1, 0.2, and 0.5 %) in three time duration levels (24, 48 and 72 hours) on ploidy induction of in vitro regenerated seedlings of the species was studied. For detection of polyploid plants, morphological, physiochemical, biochemical and cytogeneticall characteristics were analyzed. ANOVA results revealed that, the effects of colchicine concentrations on explants survival rate were significant at p < 0.01. Among the colchicine treatments the highest seedling survival rate (84.44%) was observed by 0.05% treatment. Although the most dead plants, were recorded in high colchicine concentration (0.5%). Induction of tetraploidy in Salvia Leriifolia accompanied by significant changes in different morphological, cytological, physiological and physiochemical characteristics such as, increasing length and width of stomata on leaf surface, leaf width, leaf area, leaf chlorophyll content, total antioxidant levels, total phenol and antioxidant enzyme activity (catalase and ascorbate peroxidase), as well as reducing density of stomata per unit leaf area and leaf length. Chromosome number of diploid plants was 2n = 2x = 22, and in tetraploid plants was 2n = 4x = 44. Therefore, colchicine effectively induces polyploidy in the species. | ||
| کلیدواژه‌ها [English] | ||
| colchicine, in vitro, polyploidy, Salvia leriifolia, Total antioxidant, total phenol | ||
| مراجع | ||
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- Abdoli, M., Moieni, A. and Badi, H. N., 2013. Morphological, physiological, cytological and phytochemical studies in diploid and colchicine-induced tetraploid plants of Echinacea purpurea (L.). Acta Physiologiae Plantarum, 13: 12- 22. - Adams, J. and Hansche, P. E., 1974. Population studies in microorganisms I. Evolution of diploidy in Saccharomyces cerevisiae. Genetics, 76: 327- 338. - Aebi, H., 1984. Catalase in vitro. Methods in Enzymology, 105: 121- 126. - Afshar Mohammadian, M., Omidi, Z., Porakbari, R. and Asadiabkenar, A., 2013. Effect of polyploidy on some anatomical characteristics and antioxidant compounds of sour lemon, Iranian Journal of Plant Biology, 26(3): 238-246. - Arzani, A. 2001. Breeding Field Crops. (Authored Pullman and Aysplar). Second edition. Isfahan University Publication Center, 606 pp (In Persian). - Asada, K., 1992. Ascorbate peroxidase: A hydrogen peroxide scavenging enzyme in plant. Physiologia Plantarum, 58: 235- 241. - Byrne, M. C., Nelson, C. J. and Randall, D. D., 1981. Ploidy effects on anatomy and gas exchange of tall fescue leaves. Plant Physiology, 68: 891- 893. - Chavadej, S. and Becker, H., 1984. Lnfluence of Colchicine treatment on chromosome number and growth rate of tissue Culure of Valeriana wallichii DC. Plant Cell Tiss Organ Cult. 3: 265-272 - Chen, L. L. and Gao, S. L., 2007. In vitro tetraploid induction and generation of tetraploids from mixoploids in Astragalus membranaceus. Scientia Horticulturae, 112: 339- 344. - Chiou, A., Karathanos, V. T., Mylona, A., Salta, F. N., Preventi, F. and Andrikopoulos, N. K., 2007. Currants (Vitis vinifera L.) content of simple phenolics and antioxidant activity. Food Chemistry, 102: 516- 522. - Dhawan, O. P. and Lavania, U. C., 1996. Enhancing the productivity of secondary metabolites via induced polyploidy: a review. Euphytica, 87: 81- 89. - Dwivedi, N.K.,Susheelamma, B.N., Sikdar, A.K., Suryanarayana, N., Jolly, M.S. and Sengupta, K., 1989. Induced tetraploidy in mulberry III. Morphological and hybridization studies in cultivars S30 and S36. Indian Journal Series, 28: 131-138. - Estaji, A. 2012. The effects of some environmental factors and ploidy level on morphological and physiological characteristics of Noruzak (Salvia leriifolia Benth) Dissertation, Urmia University, Iran. - Fattahi, M., Nazeri, V., Sefidkon, F., Zamani, Z. and Palazon, J., 2011. The effect of pre-sowing treatments and light on seed germination of Dracocephalum kotschyi Boiss: An endangered medicinal plant in Iran. Horticulture Environment and Biotechnology, 52: 559-566. - Gantait, S., Mandal, N., Bhattacharyya, S. and Kanti Das, K., 2011. Induction and identification of tetraploids using In vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella. Plant Cell Tissue Organ Culture, 10: 112- 227. - Gao, S. L., Zhu, D. N., Cai, Z. H. and Xu, D. R., 1996. Autotetraploid plants form colchicine-treated bud culture of Salvia miltiorrhiza Bge. Plant Cell Tissue and Organ Culture, 47: 73- 77. - Griesbach, R. and Kam, K., 1995. The effect of inducted polyploidy on the flavonols of Petunia 'mitchell. Phytochemistry, 42 (2): 361-363. - Hamill, S., Smith, M. and Dodd, W., 1992 In vitro induction of banana autotetraploids by colchicine treatment of micropropagated diploids. Australian J Botany, 40:887–896 - Hasani, M.A., Mirzaie, M., Omidbaygi, R. and Fathi Qarebaba, M., 2010. The effect of the amount of oil and some characteristics of qualitative and quantitative autotetraploid Ocimum basilicum L. Journal of Horticultural Science, 41 (2): 111-118. - Hosseinzadeh, H., Sadeghnia, H. R. and Imanshahidi, M., 2009. Review of the Pharmacological and Toxicological Effects of Salvia leriifolia. Iranian Journal of Basic Medical Sciences, 12: 1- 8. - Kang, H. M. and Saltiveit, M. E., 2002. Chilling tolerance of maize, cucumber and rice seedling (leaves and roots) and differentially affected by salicylic acid. Physiologia Plantarum, 115 (4): 571- 576. - Kondorosi, E., Roudier, F. and Gendreau, E., 2000. Plant cell-size control growing by ploidy. Current Opinion in Plant Biology, 3: 488-492. - Lavania, U. C., 1988. Enhanced productivity of the essential oil in the artificial autotetraploid of vetiver (Vetiveria zizanioides L.). Euphytica, 38: 271-276. - Lavania, U. C., 2005. Genomic and ploidy manipulation for enhanced production of phyto- pharmaceuticals. Plant Genetics Resources, 3: 170- 177. - Lavania, U. C. and Strivastava, S., 1991. Enhanced productivity of tropane alkaloids and fertility in artificial autotetraploids of Hyoscyamus niger L. Euphytic, 52: 73- 77. - Levin, D. A., 1983. Polyploidy and novelty in flowering plants. American Naturalist, 122: 1- 25. - Lichtenthaler, H. K. and Wellburn, A. R., 1985. Determination of total carotenoids and chlorophylls A and B of leaf in different solvents. Biochemical Society Transactions, 11: 591-592. - Liu, S., Chen, S., Chen, Y., Guan, Z., Yin, D. and Chen, F., 2011. In vitro induced tetraploid of Dendranthema nankingense (Nakai) Tzvel. shows an improved level of abiotic stress tolerance. Scientia Horticulturae, 127: 411- 419. - Mable, B. K., 2001. Ploidy evolution in the yeast Saccharomyces cerevisiae: A test of the nutrient limitation hypothesis. Journal of Evolutionary Biology, 14: 157–170. - Madani, H., Hosseini, B., Dehghan, E. and Rezaei-Chiyaneh, E., 2015. Enhanced production of scopolamine in induced autotetraploid plants of Hyoscyamus reticulatus L. Acta Physiologiae Plantarum, 37:55 - Majdi, M., Karimzadeh, G., Malboobi, M. A., Omidbaigi, R. and Mirzaghaderi, G., 2010. Induction of tetraploidy to feverfew (Tanacetum parthenium Schulz-Bip.): Morphological, physiological, cytological, and phytochemical changes. Horticalture Science, 45: 16- 21. - Mathura, S., Fossey, A. and Beck, S. L., 2006. Comparative study of chlorophyll content in diploid and tetraploid black wattle (Acacia mearnsii). Forestry, 79: 381- 388. - Milo, J., Levy, A., Palevitch, D. and Ladizinsky, G., 1987. The bain content and yield in induced tetraploid and triploid plants of papaver bracteatum LINDL. Euphytica, 39(2): 45- 54. - Omidbaigi, R., Mirzaee, M., Hassani, M. E. and Sedghi Moghadam, M., 2010a. Induction and identification of polyploidy in basil (Ocimum basilicum L.) medicinal plant by colchicine treatment. International Journal of Plant Production, 4: 1735- 8043. - Omidbaigi, R., Yavari, S., Hassani, M. E. and Yavari, S., 2010b. Induction of autotetraploidy in Dragonh (Dracocephalum moldavica L.) by colchicine treatments. Journal of Fruit and Ornamental Plant Research, 18(1): 23- 35. - Randall, D. D., Nelson, C. J. and Asay, K. H., 1977. Ribulose bisphosphate carboxylase altered genetic expression in tall fescue. Plant Physiology, 59: 38- 41. - Rechinger, K. H., 1982. Flora Iranica. No. 150, Graz: Akademicsch Druck- u.Verlagsannstal, Pp. 417. - Rêgo, M., Rêgo, E., Bruckner, C., Finger, F. and Otoni, W., 2011. In vitro induction of autotetraploids from diploid yellow passion fruit mediated by colchicine and oryzalin. Plant Cell Tissue and Organ Culture, 107: 451- 459. - Rowson, J.M. 1949. Increased alkaloid contents of induced polyploid of Datura. Nature Reviews Genetics, 154: 81-82. - Roy, A., Leggett, G. and Koutoulis, A., 2001. In vitro tetraploid induction and generation of tetraploids from mixoploids in hop (Humulus lupulus L.). Plant Cell Reports, 20: 489- 495. - Shahriari Ahmadi, F., Dehghan, E., Farsi, M. and Azizi, M ., 2008. Tetraploid induction of Hyoscyamus muticus L. using colchicine treatment. Pakistan Journal of Biological Sciences, 11: 2653-2659 - Sikdar, A. and Jolly, M., 1994. Induced polyploidy in mulberry (Morus spp.): induction of tetraploids. Sericologia, 34: 105-122. - Sikdar, A.K., Dwiwedi, N.K., Dandin, S.B., Kumar, R. and Giridhar, K., 1986. Stomatal chloroplast count technique as a tool to ascertain different ploidy level in mulberry. Indian Journal of series, 25: 88-90 - Slinkard, K. and Singleton, V. L., 1977. Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture, 28(1): 49- 55. - Tabatabaie Yazdi., F. 1996. Study of antioxidant Effects of essential oil and leaf extract of salvia leriifolia leriifolia and identifying its phytochemical. Master's Thesis Chemistry, College of Sciences, Mashhad Ferdowsi University, 121p. - Sotude Ardabili, G., Asgari zakaria, R., and Zare, N., 2014. Polyploid induction effect on morpho-physiological traits of Sorghum bicolor cv. KFS 2. Iranian Journal of Crop Sciences. 16(2): 151-165. - Tang, Z. Q., Chen, D. L., Song, Z. J., He, Y. C. and Cai, D. T., 2010. In vitro induction and identification of tetraploid plants of Paulownia tomentosa. Plant Cell Tissue and Organ Culture. 102: 213- 220. - Thomas, G. and Ranney, F., 2006. Polyploidy: From Evolution to New Plant Development. Combined Proceedings International Plant Propagators Society, 56: 137-142. - Wallaart T.E., Pras N. and Quax, W.J.,1999. Seasonal variations of artemisinin and its biosynthetic precursors in tetraploid Artemisia annua plants compared with the diploid wild-type. Planta Medica, 65: 723-728. - Weber, J., Georgiev, V., Pavlov A., and Bley, T., 2008. Flow cytometric investigations of diploid and tetraploid plants and in vitro cultures of Datura stramonium and Hyoscyamus niger. Cytometry Part a, 73: 931-939. - Yang, X., Cao, Z., An, L., Wang, Y. and Fang, X., 2006. In vitro tetraploid induction via colchicine treatment from diploid somatic embryos in grapevine (Vitis vinifera L.). Euphytica, 152: 217- 224. - Yavari, S., 2007. The effect of treatment with colchicine on morphological, physiological and active substances of Dracocephalum moldavica L. M.Sc. Thesis, Tarbiat Modares University, 140p. - Zahedi, A.A., 2013. The effects of colchicine on some morphological, biochemical and cytogenetic of Dracocephalum kotschyi Boiss. M.Sc. Thesis, Urmia University, 96p. - Zhang, X. Y., Hu, C. G. and Yao, J. L., 2010. Tetraploidization of diploid Dioscorea results in activation of the antioxidant defense system and increased heat tolerance. Journal of Plant Physiology, 167: 88- 94. - Zhang, Z., Dai, H., Xiao, M. and Liu, X., 2008. In vitro induction of tetraploids in Phlox subulata L. Euphytica, 159: 59-65. | ||
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