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Optimization of in vitro Culture for Gerbera cv. Tropic Blend | ||
| به زراعی نهال و بذر | ||
| Article 3, Volume 25, Issue 4, November 2010, Pages 389-401 PDF (227.18 K) | ||
| DOI: 10.22092/sppj.2017.110385 | ||
| Authors | ||
| M. Tatari Varnousefaderani* ; N. Askari Rabori; S. Z. Nosrati | ||
| Abstract | ||
| Immature capitulum of gerbera cultivar of Tropic Blend, after disinfection and removal of sepals, divided into four parts, and cultured on 1/2MS medium supplemented with 0.05, 0.1, 0.5 and 1 mg/l TDZ and 0.1 mg/l IAA. The remainings were also cultured in 1/2MS medium, supplemented with 5, 10 and 15 mg/l BAP and 1 mg/l IAA. Cultures were incubated in dark conditions and then transferred to the light. There was not any prolifration in 1/2 MS medium suplemented with different concentrations of BAP. The highest number of plantlets was obtained in 1/2 MS supplemented with 0.05 mg/l TDZ. For prolifration, plantlets were produced in MS medium supplemented with 2, 4, 6 and 8 mg/l kinetin. The highest and lowest number of plantlets were producted in MS medium with 2 and 8mg/l kinetin and 1 mgl IAA, respectively. The highest number of leaves was produced in 2 and 4 mg/l kinetin. For rooting, plantlets were cultured in MS medium supplemeted with 0.5 and 1 mg/l NAA. The highest number of roots was produced in MS with 0.5 mg/l NAA. For acclimatization the rooted plantlets were transferred into pots. They were throughly acclimized. | ||
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