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Polymorphism of TFAM gene and its association with growth traits in Sistani cattle | ||
| علوم دامی | ||
| Article 7, Volume 30, Issue 114, June 2017, Pages 65-76 PDF (997.21 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/asj.2017.111312 | ||
| Authors | ||
| Hossein Moradgholi1; Gholam Reza Dashab* 2; Mehdi Vafa Valleh3 | ||
| 1MS.c of Animal Breeding and Genetics . University of Zabol | ||
| 2Assistant professor of animal science | ||
| 3Assistant Professor, Animal Breeding and Genetics, University of Zabol | ||
| Abstract | ||
| Sistani beef cattle is a native breed of east of Iran that as a valuable genetic resource in the tropical area could be good a candidate to produce high quantity of muscular carcass portions with high efficiency. In order to explore the Mitochondrial transcription factor A gene (TFAM) polymorphisms using PCR-RFLP, 150 Sistani cows were randomly selected and then blood samples were taken from the jugular vein of each animal individually. Thereafter, blood samples were salting-out and electrophoresed with 1% agarose gel. The position of the TFAM promoter region amplified by PCR and PCR products with 801bp length were sliced by BsuRI enzyme. Digestion products on 3% agarose gel were shown by electrophoresis and staining with ethidium bromide. The patterns of digestion in AA homozygotes, CC homozygotes, and AC heterozygotes were as follows: three bands with 152, 187, and 462bp; four bands with 83, 104, 152, and 462bp; and five bands with 83, 104, 152, 187, and 462bp, respectively. The results showed that the position of TFAM population deviated from the Hardy-Weinberg equilibrium (P <0.05). Shannon index (I), Nei index, observed heterozygosity, and expected heterozygosity were 0.69, 0.49, 0.37, and 0.50, respectively. In conclusion, no significant differences between genotypes of Sistani cattle were observed for TFAM gene polymorphism and growth traits. | ||
| Keywords | ||
| Sistani cattle; Growth traits; Daily gain; TFAM gene | ||
| References | ||
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