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Isolation and Detection of Mycoplasma agalactiae from Semen Samples of Goats | ||
| Archives of Razi Institute | ||
| مقاله 2، دوره 72، شماره 3، آذر 2017، صفحه 159-164 اصل مقاله (253.46 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2017.111610 | ||
| نویسندگان | ||
| S. A. Pourbakhsh* 1؛ A. R. Abtin1؛ A. Ashtari2؛ B. Kheirkhah3؛ M. A. Bayatzadeh2؛ Salimeh Ahangran4 | ||
| 1Mycoplasma Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran | ||
| 2Master | ||
| 3Assistant Professor | ||
| 4Bachelor | ||
| چکیده | ||
| Contagious agalactia (CA) is a highly infectious disease of goats and sheep, and is a form of Mycoplasmosis, which is usually enzootic. Since Mycoplasma agalactiae (M. agalactiae) is the main cause of this disease in goats, the aim of this study was to isolate and detect M. agalactiae from semen of goat bucks. Thirty-nine semen samples were collected from goat bulks, and all samples were cultured in PPLO broth medium supplemented for M. agalaciae isolation. The bacteria DNAs were extracted from clinical samples and the PCR assay was applied to detect Mycoplasma genus and M. agalactiae species using specific primers, which amplified a 163bp fragment in 16SrRNA gene and a 375bp fragment in lipoprotein gene. The PCR evaluations were performed for both the clinical samples and the cultures. Out of the 39 samples, 29 (74.3%) of the cultures were shown positive and typical Mycoplasma colonies grew on PPLO agar, which could be considered as the diagnostic method. In addition, 38 (97.4%) samples had positive PCR results for Mycoplasma genus and six (15.3%) of the samples were shown to be positive using PCR for M. agalactiae as the diagnostic method. In the present study, M. agalactiae was detected in semen of goat bulks for the first time in Iran. Therefore, it is recommended to concern semen as one of the significant sources for this pathogen and the possibility for transmission to the female goats through semen is highlighted. Moreover, presence of this microorganism in semen could be involved in infertility of goat population. | ||
| کلیدواژه‌ها | ||
| Goat buck؛ Lipoprotein gene؛ Mycoplasma agalactiae؛ Semen؛ 16srRNA | ||
| عنوان مقاله [English] | ||
| جداسازی و شناسایی مایکوپلاسما آگالاکتیه از اسپرم بز | ||
| چکیده [English] | ||
| بیماری آگالاکسی یکی از عفونیترین بیماریهای شایع در گوسفند و بز میباشد. این بیماری از عفونتهای مایکوپلاسمایی محسوب میشود که معمولا همهگیر است. مایکوپلاسما آگالاکتیه بعنوان اصلیترین عامل این بیماری در بزها میباشد. هدف از این مطالعه جداسازی و شناسایی مایکوپلاسما آگالاکتیه از اسپرم بزهای نر بود. در مجموع 39 نمونه از اسپرم بزهای نر جمع آوری شد. تمام نمونه ها در محیط اختصاصی (PPLO broth) برای جداسازی مایکوپلاسما آگالاکتیه مورد کشت قرار گرفتند. پس از استخراج DNA باکتریها، آزمون واکنش زنجیرهای پلیمراز بر روی نمونه های استخراجی با استفاده از پرایمرهای اختصاصی با محصولاتی به طول 163 جفت باز در ژن 16S rRNA برای جنس مایکوپلاسما و 375 جفت باز در ژن لیپوپروتئین برای گونه مایکوپلاسما آگالاکتیه انجام شد. از مجموع 39 نمونه گرفته شده( %3/74)29نمونه مثبت بوده و دارای پرگنه در محیط کشت PPLOآگار بودند. (%4/97) 38 نمونه در آزمون واکنش زنجیرهای پلیمراز جنس مایکوپلاسما مثبت گزارش شدند و همچنین (%3/15)6 نمونه نیز در آزمون واکنش زنجیرهای پلیمراز گونه مایکوپلاسما آگالاکتیه مثبت تشخیص داده شدند که همگی آنها در آزمون واکنش زنجیرهای پلیمراز جنس مایکوپلاسما مثبت گزارش شده بودند. این مطالعه اولین گزارش جداسازی و شناسایی مایکوپلاسما آگالاکتیه از اسپرم بزهای نر در ایران میباشد. نتایج این مطالعه نشان داد که اسپرم میتواند محل مناسبی برای حضور این پاتو‍ژن و سپس انتقال آن به جنس ماده محسوب شود، لذا این عامل را میتوان به عنوان یکی از عوامل ناباوری در بزهای ماده محسوب نمود. | ||
| کلیدواژه‌ها [English] | ||
| مایکوپلاسما آگالاکتیه, بزهای نر, اسپرم, 16S rRNA, ژن لیپوپروتئین | ||
| مراجع | ||
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