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ارزیابی ارتباط بین چند شکلی پنج جایگاههای ریزماهوارهای واقع بر کروموزم شماره شش با صفت چند قلوزایی در بز نژاد کشمیر | ||
| علوم دامی | ||
| مقاله 23، دوره 30، شماره 115، شهریور 1396، صفحه 303-316 اصل مقاله (1.07 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2017.113283 | ||
| نویسندگان | ||
| رحمان حاجی علیزاده ولیلو1؛ آرش جوانمرد1؛ نادر اسدزاده* 2 | ||
| 1گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز | ||
| 2استادیار موسسه تحقیقات علوم دامی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی | ||
| چکیده | ||
| برای ارزیابی ارتباط بین چندشکلی پنج جایگاه ریزماهواره واقع بر کروموزم شماره شش با صفت چندقلوزایی بز نژاد کشمیر، تعداد 65 رأس بز ماده بهطور تصادفی از گله 600 رأسی انتخاب و عواملی همچون اطلاعات شجره، اثرات ثابت رکوردبرداری شده همچون سن مادر، تعداد شکم زایش به همراه تعداد فرزند در هر زایش،اطلاعات تغذیهای و اطلاعات آزمایشات فیزیولوژی تولیدمثل مانند سابقه استفاده از هورمون، بههمراه صفت هدف چندقلوزایی ثبت شد. خونگیری از ورید وداجی و استخراج DNA انجام شد. برنامه تکثیر انفرادی جایگاهها در روی ژل اکریلامید الکتروفورز و تجزیه و تحلیل شد. وجود چندشکلی در چهار جایگاه را تأیید کرد و جایگاه BM4621 الگوی مونومورف را نشان داد. رابطهی آماری چند شکلی جایگاههای مورد مطالعه با صفت تعداد نتاج در اولین سال زایش معنیدار بود (01/0>p). میانگین تعداد بزغالهی متولد شده از بزهای حاوی ژنوتیپهای 180/180و180/188 برای جایگاه BM1329، ژنوتیپهای 112/110 و 112/112برای جایگاه OarAE101 ، ژنوتیپهای 108/106 و 108/108برای جایگاه BMP143 و در نهایت ژنوتیپ 200/200 برای جایگاه BMP143 بالاتر از سایر ژنوتیپهای بود (01/0>p). روابط آماری معنیدار شناسایی شده در این مطالعه میتواند به منظور بهبود راندمان تولیدمثلی بر مبنای انتخاب به کمک نشانگر و همچنین در فرایند غربالگری برای شناسایی بزهای ماده چندقلوزا به کار گرفته شود. | ||
| کلیدواژهها | ||
| "بز"' "چندقلوزایی"؛ "نشانگر ریزماهواره"؛ "کروموزوم 6" | ||
| عنوان مقاله [English] | ||
| Association between Microsatellite Markers of Chromosome 6 and Litter size of Cashmere Goats | ||
| نویسندگان [English] | ||
| Rahman Hajializadeh Valilou1؛ Arash Javanmard1؛ nader asadzadeh2 | ||
| 1Graduated from Tabriz university | ||
| 2Assistant professor of Animal Science Research Institute of Iran | ||
| چکیده [English] | ||
| Five microsatellite markers BM1329, OarAE101, BM143, BM4621 and BM415 which were closely linked to the fecundity gene FecB in Booroola sheep were chosen based on their high conservatism in the closest species and were analyzed for polymorphisms and also their correlation with the litter size of a goat. Observed results showed four microsatellite loci show polymorphism in the present study. However, BM4621 locus was monomorphic. The range of allele size of BM1329, OarAE101, BM143 and BM415 were 180 to 216 to 112, 102 to 112 and 198 to 200 in goat respectively. The alleles of the greatest frequency for of BM1329, OarAE101, BM143 and BM415 were 212, 99, 108 and 200bp respectively. Polymorphism information content (Nei Index) for BM1329, OarAE101, BM143 and BM415 were 0.82, 0.82, 0.72 and 0.49 respectively. Mean number of allele, Observed Heterozygosis, PIC and Shannon information index were 4.75 ± 2.2, 0.36 ± 0.12, 0.71 ± 0.15 and 1.41 ± 0.52 respectively. The litter size for first lambing for investigated loci were significantly different (p<0.01). The total mean of litter size of genotype BM1329 180/180, 180/188, oarAE101 110/112, 112/112, BMp143 106/108, 108/108 and BMP143 200/200bp were significantly higher than of other genotypes. This association could be further evaluated for marker-assisted selection and developed PCR methodology would expedite screening for large number of goats required for such studies. | ||
| کلیدواژهها [English] | ||
| "Goat", " Litter Size", "Microsatellite", "Chromosome 6" | ||
| مراجع | ||
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