Kabiri, K., Tadayon, K., Pourbakhsh, S., Nowroozi, J. (2018). Phylogeny of Mycoplasma agalactiae vaccine strains in Iran, a molecular study based on multilocus sequence typing. , 31(1), 93-99. doi: 10.22092/vj.2017.111106.1324
Kh. Kabiri; K. Tadayon; S.A. Pourbakhsh; Jamileh Nowroozi. "Phylogeny of Mycoplasma agalactiae vaccine strains in Iran, a molecular study based on multilocus sequence typing". , 31, 1, 2018, 93-99. doi: 10.22092/vj.2017.111106.1324
Kabiri, K., Tadayon, K., Pourbakhsh, S., Nowroozi, J. (2018). 'Phylogeny of Mycoplasma agalactiae vaccine strains in Iran, a molecular study based on multilocus sequence typing', , 31(1), pp. 93-99. doi: 10.22092/vj.2017.111106.1324
Kabiri, K., Tadayon, K., Pourbakhsh, S., Nowroozi, J. Phylogeny of Mycoplasma agalactiae vaccine strains in Iran, a molecular study based on multilocus sequence typing. , 2018; 31(1): 93-99. doi: 10.22092/vj.2017.111106.1324

Phylogeny of Mycoplasma agalactiae vaccine strains in Iran, a molecular study based on multilocus sequence typing

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 11, Volume 31, Issue 1 - Serial Number 118, March 2018, Pages 93-99    PDF (494.71 K)
Document Type: Full Research Paper
DOI: 10.22092/vj.2017.111106.1324
Authors
Kh. Kabiri1; K. Tadayon* 2; S.A. Pourbakhsh2; Jamileh Nowroozi1
1Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran.
2Agricultural Research, Education and Extention Organization (AREEO), Razi Vaccine and Serum Research Institute, Karaj, Iran.
Abstract
Mycoplasma agalctiae (Ma) is a principle agent in etiology of agalaxy. Iran remains a major world stronghold for agalaxy with numerous annual outbreaks. Three indigenous Ma strains of Lorestan, Taliqan and Shiraz are used in preparation of agalaxy vaccine at Razi institute. In order to characterize genetic properties of these strains a MLST genotyping system consisting of five genes (dnaA, gltX, gyrB, metS, tufA) based on guidelines recently developed was employed. In consequence, at dnaA, gltX, gyrB, metS and tufA loci of all the three strains alleles 1, 21, 2, 2 and 1 were identified, respectively. The gltX allele (21) was shown to be new with no previous record in the MLST database. A new sequence type (ST33) with close similarity to ST4 was assigned to these stains. Given the characteristics of the Iranian animal farming sector, we assume circulation of more clonal complex or complexes of Ma in the Iranian environment is expectable.
Keywords
Mycoplasma agalactia; Agalaxy; MLST; Clonal complex; Sequence type
References

1. Manso-Silvan, L., et al., Phylogeny and molecular typing of Mycoplasma agalactiae and Mycoplasma bovis by multilocus sequencing. Vet Microbiol, 2012. 161(1-2): p. 104-12.
2. McAuliffe, L., et al., Multilocus sequence typing of Mycoplasma agalactiae. Journal of Medical Microbiology, 2011. 60(6): p. 803-811.
3. Sotoodehnia, A., et al., Preparation of agalactia vaccine in fermentor. Archives of Razi Institute, 2007. 62(1): p. 45-48.
4. Bory, G. and F. Entessar, Etude analytique de l'immiunopro- phylaxie de l'agalaxie contagieuse des chevres et des moutons. Arch. Inst. Razi, 1959. 11: p. 5.
5. Watson, W., et al., The pathogenicity of mycoplasma organisms isolated from sheep and goats in Turkey. Journal of Comparative Pathology, 1968. 78(3): p. 283-291.
6. Al-aubaidi, J.M., A.H. Dardiri, and J. Fabricant, Biochemical characterization and antigenic relationship of Mycoplasma mycoides subsp. mycoides, Freundt and Mycoplasma mycoides subsp. capri (Edward) Freundt. Int J Syst Evol Microbiol, 1972. 22(3): p. 155-164.
7. Baharsefat, M., B. Yamini, and P. Ahourai, Mycoplasma agalactiae. V. Comparison of three different contagious agalactia vaccine. Inst Razi Arch, 1971. 23: p. 6.
8. Kheirabadi, K. and A. Ebrahimi, Investigation of Mycoplasma agalactiae in milk and conjunctival swab samples from sheep flocks in west central, Iran. Pakistan Journal of Biological Sciences: PJBS, 2007. 10(8): p. 1346-1348.
9. Kheirkhah, B., et al., The molecular characterization of Mycoplasma agalactiae isolates from Iranian goats when compared with other Iranian isolates and vaccinal strains. African Journal of Microbiology Research, 2013. 7(4): p. 324-329.
10. Khezri, M. and S. Pourbakhsh, A survey of Mycoplasma agalactiae in small ruminants with contagious agalactiae syndrome in Iran. Bangladesh Journal of Veterinary Medicine, 2014. 12(1): p. 67-72.
11. Nouvel, L.X., et al., Molecular typing of Mycoplasma agalactiae: tracing European-wide genetic diversity and an endemic clonal population. Comp Immunol Microbiol Infect Dis, 2012. 35(5): p. 487-96.
12. Nouvel, L.X., et al., Comparative genomic and proteomic analyses of two Mycoplasma agalactiae strains: clues to the macro- and micro-events that are shaping mycoplasma diversity. BMC Genomics, 2010. 11: p. 86.
13. McAuliffe, L., R.D. Ayling, and R.A. Nicholas, Identification and characterization of variable-number tandem-repeat markers for the molecular epidemiological analysis of Mycoplasma mycoides subspecies mycoides SC. FEMS Microbiol Lett, 2007. 276(2): p. 181-8.
14. Maiden, M.C., et al., Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc Natl Acad Sci USA, 1998. 95(6): p. 3140-5.
15. Jolley, K.A., M.S. Chan, and M.C.J. Maiden, MLSTdbNet–distributed multi-locus sequence typing (MLST) databases. BMC Bioinformatics, 2004. 5(1): p. 86.
16. Subramaniam, S., et al., Species identification of Mycoplasma bovis and Mycoplasma agalactiae based on the uvrC genes by PCR. Mol Cell Probes, 1998. 12(3): p. 161-9.
17. Stucky, B.J., SeqTrace: a graphical tool for rapidly processing DNA sequencing chromatograms. J Biomol Tech, 2012. 23(3): p. 90-3.
18. Li, W., et al., The EMBL-EBI bioinformatics web and programmatic tools framework. Nucleic Acids Res, 2015. 43(W1): p. W580-4.
19. Manso-Silván, L., et al., Phylogeny and molecular typing of Mycoplasma agalactiae and Mycoplasma bovis by multilocus sequencing. Vet Microbiol, 2012. 161(1): p. 104-112.
20. Mayor, D., et al., Multilocus sequence typing (MLST) of Mycoplasma hyopneumoniae: a diverse pathogen with limited clonality. Vet Microbiol, 2008. 127(1-2): p. 63-72.
21. Tocqueville, V., et al., Multilocus sequence typing of Mycoplasma hyorhinis strains identified by a real-time TaqMan PCR assay. J Clin Microbiol, 2014. 52(5): p. 1664-71.
22. Brown, R.J., et al., Development of a multilocus sequence typing scheme for molecular typing of Mycoplasma pneumoniae. J Clin Microbiol, 2015. 53(10): p. 3195-203.
23. El-Gazzar, M., et al., Development of Multilocus Sequence Typing (MLST) for Mycoplasma synoviae. Avian Dis, 2017. 61(1): p. 25-32.
24. Mahdavi, S., et al., Comparative study of homology of cytoplasmic membrane protein 40 KDa of Mycoplasma agalactiae in isolated strains in Iran. African Journal of Microbiology Research, 2009. 3(9): p. 528-532.

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