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تأثیر تیمارهای مختلف فرسودگی بذر و جوانه زنی در دماهای مختلف بر فعالیت آنزیم های آنتی اکسیدانت و پراکسیداسیون لیپیدی گیاهچه های کدوی تخم کاغذی | ||
| علوم و فناوری بذر ایران | ||
| مقاله 17، دوره 6، شماره 1 - شماره پیاپی 11، خرداد 1396، صفحه 205-218 اصل مقاله (1.32 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2017.113928 | ||
| نویسندگان | ||
| صغری قهرمانی* 1؛ محمد صدقی2؛ رئوف سید شریفی3 | ||
| 1دانش آموخته کارشناسی ارشد رشته علوم وتکنولوژی بذر/گروه زراعت و اصلاح نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران. | ||
| 2عضوهیئت علمی/ گروه زراعت واصلاح نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران. | ||
| 3عضو هیئت علمی/گروه زراعت واصلاح نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران. | ||
| چکیده | ||
| به منظور بررسی اثرات فرسودگی بذر بر میزان فعالیت آنزیم های آنتی اکسیدانت و پراکسیداسیون لیپیدی در گیاهچه های کدوی تخم کاغذی آزمایشی به صورت فاکتوریل در قالب طرح کاملا تصادفی در سه تکرارانجام گرفت. تیمارهای آزمایش شامل سه سطح فرسودگی (شاهد، 5 و 10 روز فرسودگی در دمای 45 درجه سانتیگراد با رطوبت نسبی 95 تا 100 درصد( و سه سطح دماهای جوانه زنی (15، 25 و 35 درجه) بود. نتایج حاصل نشان داد که آنزیمهای سوپراکسیددیسمیوتاز و کاتالاز تحت تأثیر فرسودگی 5 روز و در دمای 25 درجه و پراکسیداز در تیمار شاهد و در دمای 25 درجه بیشترین فعالیت را داشتند. همچنین، بیشترین میزان مالون دی آلدئید (1/7 میلی مول بر گرم وزن تر) در دمای 15 درجه و در بذور 10 روز فرسوده مشاهده شد. بیشترین مقدار ماده خشک مصرف شده در تنفس (0.018 میلی گرم) از تیمار 5 روز فرسودگی و دمای 35 درجه حاصل شد و کمترین (0.009 میلی گرم) آن نیز مربوط به 10 روز فرسودگی و دمای 35 درجه بود. به نظر می رسد که کاهش قدرت بذر بر اثر فرسودگی و دمای بالا منجر به تنش اکسیداتیو و پراکسیداسیون لیپیدی شده و گیاه برای مقابله با آسیب های ناشی از آن میزان فعالیت آنزیمهای آنتیاکسیدانت را افزایش داده است. با توجه به نتایج حاصل از شاخص های جوانه زنی و آنزیم های آنتی اکسیدانت دمای 15 درجه بهترین دما برای جوانه زنی بذرهای فرسوده این گیاه است | ||
| کلیدواژه‌ها | ||
| آنزیم؛ پراکسیداسیون؛ دما؛ فرسودگی | ||
| عنوان مقاله [English] | ||
| Effect of different seed deterioration treatments and germination under different temperatures on the activity of antioxidant enzymes and lipid peroxidation in Pumpkin (Cucurbita pepo L.) seedlings | ||
| نویسندگان [English] | ||
| S. Ghahremani1؛ M. Sedghi2؛ R. Seyed Sharifi3 | ||
| 1Former M.Sc. student of Seed Science and Technology/Dept. of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh ardabilli, Ardabil, Iran. | ||
| 2Prof./ Dept. of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabilli, Ardabil, Iran. | ||
| 3Prof./ Dept. of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabilli, Ardabil, Iran. | ||
| چکیده [English] | ||
| In order to investigate the effects of seed deterioration on the activity of antioxidant enzymes and lipid peroxidation in pumpkin seedlings a factorial experiment conducted based on completely randomized design with three replications. Treatments were three deterioration levels (control, 5 and 10 days deterioration at 45◦C and 95-100% relative humidity) and three germination temperatures (15, 25 and 35°C). Results showed that activity of superoxide dismutase and catalase were the highest at 5 days aging and 25°C, and activity of peroxidase was the highest at non-aging and 25°C. Also, the highest amount of Malondialdehyde (7.1 mmol gr-1FW) observed in the seeds with 10 days deterioration and 15°C. The highest respiration index (0.018 mg) achieved in 5 days deterioration and 35°C and the lowest value (0.009 mg) was related to 10 days deterioration and 35°C. It seems that reduction in seed vigor under deterioration and high temperatures causes to oxidative stress and lipid peroxidation and leads to increase in the activity of antioxidant enzymes in order to face the related damages. Considering results obtained from germination and antioxidant enzymes activity the best temperature for germination of aged seeds of pumpkin was 15◦C. | ||
| کلیدواژه‌ها [English] | ||
| Enzyme, peroxidation, temperature, deterioration | ||
| مراجع | ||
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