اخبار و اعلانات

 آمار

تعداد نشریات283
تعداد نشریات سازمان81
تعداد شماره‌ها2,726
تعداد مقالات31,041
تعداد مشاهده مقاله27,909,329
تعداد دریافت فایل اصل مقاله16,235,141
Nematzadeh, M., Rezvani, S., Khalesi, M.K., Laloei, F., Fahim, A.. (1392). A phylogeny analysis on six mullet species (Teleosti: Mugillidae) using PCR-sequencing method. سامانه مدیریت نشریات علمی, 12(3), 669-679. doi: 10.22092/ijfs.2018.114307
M. Nematzadeh; S. Rezvani; M.K. Khalesi; F. Laloei; A. Fahim. "A phylogeny analysis on six mullet species (Teleosti: Mugillidae) using PCR-sequencing method". سامانه مدیریت نشریات علمی, 12, 3, 1392, 669-679. doi: 10.22092/ijfs.2018.114307
Nematzadeh, M., Rezvani, S., Khalesi, M.K., Laloei, F., Fahim, A.. (1392). 'A phylogeny analysis on six mullet species (Teleosti: Mugillidae) using PCR-sequencing method', سامانه مدیریت نشریات علمی, 12(3), pp. 669-679. doi: 10.22092/ijfs.2018.114307
Nematzadeh, M., Rezvani, S., Khalesi, M.K., Laloei, F., Fahim, A.. A phylogeny analysis on six mullet species (Teleosti: Mugillidae) using PCR-sequencing method. سامانه مدیریت نشریات علمی, 1392; 12(3): 669-679. doi: 10.22092/ijfs.2018.114307

A phylogeny analysis on six mullet species (Teleosti: Mugillidae) using PCR-sequencing method

Iranian Journal of Fisheries Sciences
مقاله 14، دوره 12، شماره 3، مهر 2013، صفحه 669-679
شناسه دیجیتال (DOI): 10.22092/ijfs.2018.114307
نویسندگان
M. Nematzadeh* ؛ S. Rezvani؛ M.K. Khalesi؛ F. Laloei؛ A. Fahim
چکیده
In this study, genetic differences and phylogenic relationships among six Mugilidae species (Mugil cephalus, M. capito, Liza subviridis, L. saliens, L. aurata, Valamugil buchanani) were determined using PCR-sequencing. M. cephalus, L. subviridis, and V. buchanani from the Persian Gulf and Oman Sea, and L. aurata and L. saliens from the Caspian Sea were col-lected. Samples of an imported, Egyptian species M. capito were obtained from the Gomishan Research Center in Gorgan. Total DNA from the samples were extracted according to phenol-chloroform procedure. The extracted total DNAs were amplified using polymerase chain reaction (PCR) and then sequenced. The number of bases in the mitochondrial 16s rRNA genome used in this study approximated 600 base pairs. The size of the bands was identical in all the studied species and no heteroplasmia was observed. In addition, the numbers of variable, preserved, and Pi sites were about 114/624, 488/624, and 110/624, respectively. Analysis of the sequences showed great differences between Mugil species and the other studied species. The phylogenetic tree obtained through Neighbor-Joining method revealed that L. saliens and L. aurata were in the same branch while L. subviridis was in a separate branch. In contrast, Maximum Parsimony tree located L. subviridis and L. aurata in a single branch and assigned L. saliens to a distinct branch. This result brings in the question of monophyletic origin of the genus Liza.
کلیدواژه‌ها
Sequencing؛ Mugillidae؛ phylogeny؛ PCR؛ Persian Gulf؛ Oman Sea؛ Caspian Sea
آمار
تعداد مشاهده مقاله: 133


counter
سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب