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Gozari, M., Mortazavi, M.S., Bahador, N., Tamadoni jahromi, S., Rabbaniha, M.. (1395). Isolation and screening of antibacterial and enzyme producing marine actinobacteria to approach probiotics against some pathogenic vibrios in shrimp Litopenaeus vannamei. سامانه مدیریت نشریات علمی, 15(2), 630-644. doi: 10.22092/ijfs.2018.114559
M. Gozari; M.S. Mortazavi; N. Bahador; S. Tamadoni jahromi; M. Rabbaniha. "Isolation and screening of antibacterial and enzyme producing marine actinobacteria to approach probiotics against some pathogenic vibrios in shrimp Litopenaeus vannamei". سامانه مدیریت نشریات علمی, 15, 2, 1395, 630-644. doi: 10.22092/ijfs.2018.114559
Gozari, M., Mortazavi, M.S., Bahador, N., Tamadoni jahromi, S., Rabbaniha, M.. (1395). 'Isolation and screening of antibacterial and enzyme producing marine actinobacteria to approach probiotics against some pathogenic vibrios in shrimp Litopenaeus vannamei', سامانه مدیریت نشریات علمی, 15(2), pp. 630-644. doi: 10.22092/ijfs.2018.114559
Gozari, M., Mortazavi, M.S., Bahador, N., Tamadoni jahromi, S., Rabbaniha, M.. Isolation and screening of antibacterial and enzyme producing marine actinobacteria to approach probiotics against some pathogenic vibrios in shrimp Litopenaeus vannamei. سامانه مدیریت نشریات علمی, 1395; 15(2): 630-644. doi: 10.22092/ijfs.2018.114559

Isolation and screening of antibacterial and enzyme producing marine actinobacteria to approach probiotics against some pathogenic vibrios in shrimp Litopenaeus vannamei

Iranian Journal of Fisheries Sciences
مقاله 3، دوره 15، شماره 2، تیر 2016، صفحه 630-644    اصل مقاله (329.58 K)
شناسه دیجیتال (DOI): 10.22092/ijfs.2018.114559
نویسندگان
M. Gozari* ؛ M.S. Mortazavi؛ N. Bahador؛ S. Tamadoni jahromi؛ M. Rabbaniha
چکیده
The application of new probiotics is a good strategy in the biological control of infectious diseases in aquaculture. Approximately 100 marine actinobacteria isolates were obtained from 10 sediment samples of shrimp farms. Heat treatment of sediment samples resulted in a selective reduction of the non actinobacterial heterotrophic microflora. Starch nitrate agar medium exhibited more efficacy than glycerol arginine agar medium for isolation. Twenty seven percent of actinobacterial isolates showed antagonistic activities against pathogenic Vibrio spp < em>. All the antagonistic isolates showed the typical morphology of genus Streptomyces. Exoenzymatic activity screening showed that 44 %, 26%, 37% of antagonistic isolates represented amylase, lipase and protease activities, respectively. MNM-1400 strain exhibited highest antagonistic and exoenzymatic activity. The pathogenicity experiment revealed that MNM-1400 strain did not cause disease in Litopenaeus vannamei larvae. Extraction of produced antibacterial compounds by MNM-1400 strain showed that the active constituent didn’t have non polar property. Morphological, physiological and biochemical identification confirmed that MNM-1400 strain belonged to the genus streptomyces. Phylogenetic analysis by 16S rRNA gene sequencing showed a high similarity between MNM-1400 strain and Streptomyces californicus (similarity: 99%). These results suggest that the MNM-1400 strain can be considered as a potential probiotic in aquaculture
کلیدواژه‌ها
Probiotic؛ Marine actinobacteria؛ biological control؛ Shrimp؛ Litopenaeus vannamei
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تعداد مشاهده مقاله: 235
تعداد دریافت فایل اصل مقاله: 238


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