Bacterial diversity in the intestine of sea cucumber  Stichopus japonicus

	Bacterial diversity in the intestine of sea cucumber Stichopus japonicus
Iranian Journal of Fisheries Sciences
مقاله 23، دوره 17، شماره 1، فروردین 2017، صفحه 318-325 اصل مقاله (140.79 K)
شناسه دیجیتال (DOI): 10.22092/ijfs.2018.114658
نویسندگان
M.L. Gao؛ H.M. Hou^* ؛ G.L. Zhang؛ Y. Liu؛ L.M. Sun
چکیده
The intestinal bacterial diversity of Stichopus japonicus was investigated using 16S ribosomal RNA gene (rDNA) clone library and Polymerase Chain Reaction/Denaturing Gradient Gel Electrophoresis (PCR-DGGE). The clone library yielded a total of 188 clones, and these were sequenced and classified into 106 operational taxonomic units (OTUs) with sequence similarity ranging from 88 to 100%. The coverage of the library was 77.4%, with approximately 88.7% of the sequences affiliated to Proteobacteria. Gammaproteobacteria and Vibrio sp. were the predominant groups in the intestine of S. japonicus. Some bacteria such as Legionella sp., Brachybacterium sp., Streptomyces sp., Propionigenium sp. and Psychrobacter sp were first identified in the intestine of sea cucumber
کلیدواژه‌ها
Intestinal bacterial diversity؛ 16S rDNA؛ PCR-DGGE؛ Sequencing؛ Stichopus japonicus

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