emam, M., Shahrzad, S., Naraghi, T., Khanhasani, M., Hamzepoor, Y. (2006). Regeneration of Acer cinerasens through Embryo culture. , 14(3), 170-174. doi: 10.22092/ijrfpbgr.2006.115065
mitra emam; S Shahrzad; T.S. Naraghi; M. Khanhasani; Y. Hamzepoor. "Regeneration of Acer cinerasens through Embryo culture". , 14, 3, 2006, 170-174. doi: 10.22092/ijrfpbgr.2006.115065
emam, M., Shahrzad, S., Naraghi, T., Khanhasani, M., Hamzepoor, Y. (2006). 'Regeneration of Acer cinerasens through Embryo culture', , 14(3), pp. 170-174. doi: 10.22092/ijrfpbgr.2006.115065
emam, M., Shahrzad, S., Naraghi, T., Khanhasani, M., Hamzepoor, Y. Regeneration of Acer cinerasens through Embryo culture. , 2006; 14(3): 170-174. doi: 10.22092/ijrfpbgr.2006.115065

Regeneration of Acer cinerasens through Embryo culture

تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران
Article 7, Volume 14, Issue 3 - Serial Number 25, November 2006, Pages 170-174    PDF (257.79 K)
Document Type: Research Paper
DOI: 10.22092/ijrfpbgr.2006.115065
Authors
mitra emam* 1; S Shahrzad2; T.S. Naraghi2; M. Khanhasani3; Y. Hamzepoor4
1Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran. E-mail: memam@rifr-ac.ir
2Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran.
3Research Center of Agricultural and Natural Resources of Kermanshah Province, Iran.
4Research Center of Agricultural and Natural Resources of Fars Province, Iran.
Abstract
Acer cinerasens is the most important and useful species of Aceraceae. Regarding difficulties in conventional propagation of hardwood plants, specially rooting of cutting in adult Acer trees, this study was carried out. To investigate in vitro culture of Acer cinerasen, seeds were gathered in different seasons from selected mature trees at Sarchahan forest in Shiraz. For culture of mature embryos, seeds were washed with running tap water for 2 days and then surface sterilized with 0.1 % (W/V) mercuric chloride. The embryos were aseptically dissected and transferred in a modified MS medium. Seedling emergence after 4 weeks ranged from 35%. After acclimatization, seedlings were transferred to soil under green house condition. Regarding difficulties in conventional propagation of Acer, specially Acer cinerasens, this study led to production of plants through embryo culture that will be important.
Keywords
Acer cinerasens; In vitro culture and Embryo culture
References

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