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Cytogenetic studies in alfalfa (Medicago sativa L.) somaclones following tissue culture | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| Article 7, Volume 13, Issue 2 - Serial Number 20, July 2006, Pages 209-220 PDF (1.21 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/ijrfpbgr.2005.115176 | ||
| Authors | ||
| Abbas Safarnejad* 1; S. Marvian2 | ||
| 1Faculty member of Khorasan Razavi Agricultural and Natural Resources Research Center or Mashhad Branch, Education and Extension Organization (AREEO) | ||
| 2Khorasan Agriculture and Natural Resources Research Center P.o Box 1148-91735, Mashhad, Iran. | ||
| Abstract | ||
| Alfalfa (Medicago sativa L.) grows extensively in farms and rangelands. In order to increase in vitro plant regeneration and salt tolerance in alfalfa (Medicago sativa L.), plants were regenerated from CUF101-1S genotype and seeds were produced. Chromosomes morphology was studied in three genotypes CUF101-1S, Reg1S and Reg2S of Medicago, using their root meristem after fixation, hydrolysis and staining stages. The result showed that all three genotypes were 2n=4x=32. Karyotypic formula was 8SM+ 7M + 1 AC in CUF101-1S and were 12 M + 4SM in Reg2S. Total chromosomes length in CUF101-1S was 31.6 and the longest and the shortest chromosomes lengths were 3.2 and 1.2, respectively. These values were stimated 31.2, 3, 19 and 25.9, 2.4, 16 for Reg1S and Reg2S, respectively. | ||
| Keywords | ||
| Medicago sativa L; cytogenetic; Tissue culture and Somaclone | ||
| References | ||
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