Arab, R., Jafari mofid abadi, A., Majd, A., Dehghan-Shoreki, Y. (2003). In vitro proliferation of date palm (Phoneix dactylifera L. cv. Mazafeti) plantlet derived from embryo culture. , 11(2), 171-180. doi: 10.22092/ijrfpbgr.2003.115495
R. Arab; A. Jafari mofid abadi; A. Majd; Y. Dehghan-Shoreki. "In vitro proliferation of date palm (Phoneix dactylifera L. cv. Mazafeti) plantlet derived from embryo culture". , 11, 2, 2003, 171-180. doi: 10.22092/ijrfpbgr.2003.115495
Arab, R., Jafari mofid abadi, A., Majd, A., Dehghan-Shoreki, Y. (2003). 'In vitro proliferation of date palm (Phoneix dactylifera L. cv. Mazafeti) plantlet derived from embryo culture', , 11(2), pp. 171-180. doi: 10.22092/ijrfpbgr.2003.115495
Arab, R., Jafari mofid abadi, A., Majd, A., Dehghan-Shoreki, Y. In vitro proliferation of date palm (Phoneix dactylifera L. cv. Mazafeti) plantlet derived from embryo culture. , 2003; 11(2): 171-180. doi: 10.22092/ijrfpbgr.2003.115495

In vitro proliferation of date palm (Phoneix dactylifera L. cv. Mazafeti) plantlet derived from embryo culture

تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران
Article 1, Volume 11, Issue 2 - Serial Number 12, July 2003, Pages 171-180    PDF (323.98 K)
Document Type: Research Paper
DOI: 10.22092/ijrfpbgr.2003.115495
Authors
R. Arab; A. Jafari mofid abadi; A. Majd; Y. Dehghan-Shoreki
Abstract
In order to determine in vitro regeneration potential of date palm (Phoneix dactylifera L. cv. Mazafati), isolated embryos from immature fruits were transferred to hormone free half-concentration MS medium and MS containing growth regulator hormone. To stimulate meristemic cells for adventitious bud initiation, isolated explants in 1 to 3 cm height were vertically cultured on MS medium containing 0.4 mg/l thiamine Hcl, 100 mg/l inositol 30 g/l sucrose and different combination of growth regulators. MS medium containing 10 mg/1 of 2ip produces highest percentage of bud development and shoot growth.
Adding MS hormone free liquid medium on surface of establishment medium with initiated bud was caused in vitro shoot elongation. Elongated shoots was used for shoot proliferation by permanent cutting of shoot in collar and transferring of them to rooting or shoot elongation media. Highest root induction was observed in MS medium supplemented with 10 mg/l 2iP and 0.01 mg/l IAA ( more than 80%).
Keywords
Date palm (Phoneix dactylifera L. cv. Mazafeti); Tissue culture; embryo culture; Micropropagation; Genetic manipullation
References

- Al-Marri, K., Bautista, C.M. and Al-Ghamdi, A.S., 1989. Micropropagation of Date Palm (Phoenix dactylifera L) cultivar "Hillaly": Factors effecting root formation in vitro. Hort. Dep. College of Agri. Sciences, King Faisal University. pp. 173-191.

- Beauchesne, G., 1982. Vegetative propagation of data palm (Phoenix dactylifera L.) by in vitro culture. Proceeding of the First Symposium on date palm. King. Faisal University Saudia Arabia 698-9.

- Cutter, V.M. and Wilson, K.S., 1954. Effect of coconut endosperm and other growth stimulants upon the development in vitro of embryos of Cocos nucifera. Botanical Gazette 115:234-240.

- Kiss, E., Kiss J., Gyulai, G. and Heszky, L.E., 1994. A novel method for rapid micropropagation of pineapple. Acta. Bio. Hungarica 12:32-41.

- Murashige, T. and Skoog, F., (1962). A revised medium for rapid growth and bioassays with Tobacco tissue cultures. Physiol. Plant. 15:473-479.

- Mater, A.A., 1983. Plant regeneration from callus cultures of Phoenix dactylifera L. Date plam Journal 2:57-77.

- Reuveni, O., 1979. Embryogenesis and plantlet growth of date palm (Phoenix dactylifera L.) derived from callus tissue. Plant Physiolgy suppl. 63.138.

- Reynolds, J.F., 1979. Morphogenesis of palms in vitro. In vitro 15:210

- Sharma, D.R., Chowdury, J.B.,  Neelman, R.Y. and Chowdury, V.K., 1990. In vitro multiplication of female date palm (Phoenix dactylifera L.). Bulletin de la Socieie Botanique de France. 137:15-53.

- Sharma, D.R., Deepak S. and Chowdury J. 1986. Regeneration of plantlets from somatic tisssues of date palm (Phoenix dactylifera L.). Indian J. of Experimantal Biology. 24:763-766.

- Sharma, D.R., Dawra S. and Chowdury J.B. 1984. Somatic embryogenesis and plant regeneration in date palm. Throgh tissue culture. Indian J. of Experimental Biology. 22:596-8.

- Sharma, D.R., Kumar, R., and Chowdury, J.B. 1980. In vitro culture of Female date palm tissues. Euphytica 29:169-174.

- Tisserat, B., 1979. Propagation of date palm (Phoenix dactylifera L.) in vitro. J. Of Experimental Botany. 30: 1275-83.

- Tisserat, B., 1982. Factors involved in the production of plantlets from date palm callus cultures. Euphytica. 31.201-214 .

- Tisserat, B., 1990. Clonall propagation of palms . In . Plant Tissue Culture Manual C2. (Lindesy K. Ed). Kluver Acadaemic Publishers, Netherlands. 1-14.

- Tisserat, B. and Demason, D.A., 1980. A histological study of development of adventive embryos in organ cultures of Phoenix dactylifera L. Date palm Journal 2:163-82.

- Veramendi, J. and Navarro, L., 1997. Influence of explant sources of adult date palm (Phoenix dactylifera L.) on embryogenic callus formation. J. of Horticultural Science 72:665-671.

- Zaid, A. and Tisserat, B., 1983. Morphogenetic responses obtained from a variety of somatic explant tissues of date palms. Botanical Magazine 96:67-73.

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