Detection of different milk in camel milk by PCR method

	Detection of different milk in camel milk by PCR method
علوم و فنون دامپروری
Article 1, Volume 6, Issue 24, December 2017, Pages 3-8 PDF (635.81 K)
DOI: 10.22092/aasrj.2017.115828
Authors
mohsen najafi^* ; R. Tafaghodi; M. Azizi; B. Majidi; F. Fathi Najafi
Assistant Professor, Razi vaccine and serum Research Department , Khorasan Razavi Agricultural and Natural Resources Research Center, AREEO
Abstract
Camel milk is a very important drink with different increasing medicinal and pharmaceutical applications. According to its low accessibility and yield of production, there is a high potent field for adulteration in this valuable drink. So it is necessary to develop rapid, cost effective and sensitive methods to identify milk type. Here we develop a polymerase chain reaction (PCR) method on the basis of designed specific primers. The designed primers were confirmed by the primer blast (NCBI). DNA of different animal milks were isolated by phenol/chloroform method. To determine the identification ability of designed method, different type of milk were mixed with camel milk and the PCR test was accomplished. The results show that designed primers have the ability to detect camel milk and differentiate milk types. This technique is sensitive method for distinguish of any milk adulteration in camel milk.
Keywords
Adulteration; Sequence alignment; Specific primers

References

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