| تعداد نشریات | 284 |
| تعداد نشریات سازمان | 82 |
| تعداد شماره‌ها | 3,049 |
| تعداد مقالات | 34,082 |
| تعداد مشاهده مقاله | 46,536,244 |
| تعداد دریافت فایل اصل مقاله | 77,586,105 |
Diagnosis of Avian Mycoplasmas: A Comparison between PCR and Culture Technique | ||
| Archives of Razi Institute | ||
| مقاله 9، دوره 73، شماره 3، آذر 2018، صفحه 239-244 اصل مقاله (218.58 K) | ||
| نوع مقاله: Short Communication | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2017.108217.1085 | ||
| نویسندگان | ||
| F. Muhammad* 1، 2؛ J. Hussain1؛ S. K. Fareed1؛ T. Ahmad Khan3؛ S. Ahmad Khan1، 4؛ A. Ahmad1، 4 | ||
| 1Department of Microbiology, Faculty of Science, University of Karachi, Karachi, Pakistan | ||
| 2Department of Microbiology, Faculty of Life Sciences & Informatics Balochistan University of IT, Engineering & Management Sciences (BUITEMS), Quetta Takkatu Campus, Airport road Quetta, Balochistan, Pakistan | ||
| 3Department of Physiology, Faculty of Science, University of Karachi, Karachi, Pakistan | ||
| 4Department of Biosciences, Faculty of Science, Barrett Hodgson University, Karachi, Pakistan | ||
| چکیده | ||
| Mycoplasma gallisepticum and Mycoplasma synoviae are the causative agents of avian mycoplasmosis in commercial poultry. Among the available tools, polymerase chain reaction (PCR) and culture are confirmatory tools for the diagnosis of mycoplasmosis after the initial serological screening of suspected birds. Overall, 181 samples were analyzed, 152 (84%) and 103 (57%) of which were found positive by PCR and culture, respectively. Further, 54 (92%) broiler samples were found positive for general avian mycoplasma. Among the total positive samples, MS positivity was as high as 72 (47%) by PCR, while it was 45 (44%) by culture. MG positivity was 23% and 25% in PCR- and culture-positive samples. MG grows more easily compared to MS. The agreement value between the tests was 67%. Overall, flock wise prevalence was not much varied. The prevalence of mycoplasmosis was higher during winter. Our study confirmed that PCR is the most sensitive and reliable tool for the diagnosis of avian mycoplasmosis in field samples. | ||
| کلیدواژه‌ها | ||
| Avian Mycoplasmosis؛ Mycoplasma gallispticum؛ Mycoplasma synoviae؛ PCR؛ Culture | ||
| عنوان مقاله [English] | ||
| تشخیص مایکوپلاسمای طیور: مقایسه بین روش واکنش زنجیره ای پلیمراز و روش کشت | ||
| نویسندگان [English] | ||
| فیض محمود1، 2؛ جوهر حسین1؛ سید خرم فرید1؛ تاثیر احمدخان3؛ شکیل احمدخان1، 4؛ عقیل احمد1، 4 | ||
| مراجع | ||
|
Bradbury, J.M., Abdul-Wahab, O.M.S., Yavari, A., Dupiellet, J.P., Bove, J.M., 1993. Mycoplasmas imitans sp.nov. Is related to Mycoplasma gallisepticum and founds in birds. Int J Sys Bacteriol 43, 721-728.
Brown, D.R., Whitcomb, R.F., Bradbury, J.M., 2007. Revised minimal standards for description of new species of the class Mollicutes (division Tenericutes). Int J Syst Evol Microbiol 57, 2703-2719.
Buim, M.R., Mettifogo, E., Timenetsky, J., Kleven, S., Ferreira, A.J.P., 2009. Epidemiological survey on Mycoplasma gallisepticum and M. synoviae by multiplex PCR in commercial poultry. Pesq Vet Bras 29, 552-556.
Elbehiry, A., Al-Dubaib, M., Marzouk, E., 2016. Serological, rapid molecular characterization and antibiotic resistance for field isolates of Mycoplasma gallisepticum n chicken in Saudi Arabia. Alexandria J Vet Sci 49, 70-79.
Feberwee, A., Vries, T.S., Landman, W. J. M., 2008. Seroprevalance of Mycoplasma synoviae in Dutch commercial poultry farms. Avian Pathol 37, 629-633.
Feberwee, A., de Wit, J.J. Landman, W. J. M., 2009. Induction of eggshell apex abnormalities by Mycoplasma synoviae: field and experimental studies. Avian Pathol 38, 77-85.
Frey, M.L., Hanson, R.P., Anderson, D.P., 1968. A medium for the isolation of avian mycoplasmas. Am J Vet Res 29, 2163-2171.
Garcia, M., Ikuta, N., Levisohn, S., Kleven, S.H. 2005. Evaluation and comparison of various PCR methods for detection of Mycoplasma gallisepticum infection in chicken. Avian Dis 49, 125-132.
Hossam, M., Waglh, A., Mona, E., Mahmoud, E., Rehab, E., and Salah, E.D.T., 2016. The recovery and molecular diagnosis of Mycoplasma gallisepticum infection in commercial poultry flocks in Egypt. Indian J Sci Tech 9, 1-8.
Kahya, S., Yilmaz, O., Eyiogr, A., Temelli, S., Carli, and K.T., 2015. Detection of Mycolasma gallisepticum and M. synoviae by real-time PCR and Mycoplasma gallisepticum-antibody detection by an ELISA in chicken breeder flocks. Kafkas Univ Vet Fak Derg 21, 361-366.
King, D.D., Kleven, S.H., Wenger, D.M., Anderson, D.P., 1973. Field studies with Mycoplasma synoviae. Avian Dis 17, 722-726.
Kleven, S.H., 2008. Control of avian mycoplasma infection in commercial poultry. Avian Dis 52, 367-374.
Landman, W.J.M., 2014. Is Mycoplasm synoviae outrunning Mycoplasm gallisepticum? A viewpoint from the Netherlands. Avian Pathol 43, 2-8.
Lauerman, L.H., Hoerr, F.J. Sharpton, A.R., Shah, S.M., Van Santen, V.L., 1993. Development and application of a polymerase chain reaction assay for Mycoplasma synoviae. Avian Dis 37, 829-834. Luciano, R.L., Cardoso, A.L.S.P., Stoppa, G.F.Z. Kanashiro, A.M.I., deCastro, A.G.M., Tessari, E.N.C., 2012. Comparative study of serological tests for Mycoplasma synoviae diagnosis in commercial poultry breeders. Vet Med Inter 20, 11-15.
Mohammed, H.O., Carpenter, T.E., Yamamoto, R., 1987. Economic impact of Mycoplasma gallisepticum and M. synoviae in commercial layer flocks. Avian Dis 31, 477-482. Marois, C., Dufour-Gesbert, F., Kempf, I., 2002. Polymerase chain reaction for detection of Mycoplasma gallisepticum in environmental samples. Avian Pathol 31, 163-168.
Stipkovits, L., Kempf, I., 1996. Mycoplasmoses in poultry. Rev Sci Tech Off Int Epiz 15, 1495-1525.
Sun, Y., Lin, L., Chen, Z., Wu, L., Xiong, Z.L., Li, X. M., 2014. Serology study of Mycoplasma gallisepticum in broiler chickens in Chon Qing. J Anim Vet Adv 13, 5-8. | ||
|
آمار تعداد مشاهده مقاله: 3,078 تعداد دریافت فایل اصل مقاله: 3,441 |
||