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Molecular Detection of Mycoplasma synoviae from Backyard and Commercial Turkeys in Some Parts of Iran | ||
| Archives of Razi Institute | ||
| مقاله 1، دوره 73، شماره 2، شهریور 2018، صفحه 79-85 اصل مقاله (253.68 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2018.116615 | ||
| نویسندگان | ||
| ُS. Rasoulinezhad1؛ M. H. Bozorgmehrifard* 1؛ H. Hosseini2؛ N. Sheikhi1؛ S. Charkhkar1 | ||
| 1Department of Poultry and Obstetrics, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Clinical Science, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran | ||
| چکیده | ||
| M. synoviae (MS) is an economically important pathogen and the major cause of airsacculitis and infectious synovitis in turkeys. Infection with this pathogen may remain asymptomatic but can render infected birds susceptible to secondary infections. This study was carried out for the molecular detection of MS infection in commercial and backyard turkey flocks in Tehran, Semnan, Isfahan, Qazvin, Zanjan, East Azerbaijan, Gilan, Mazandaran, and Golestan provinces of Iran. Sixty-hundred tracheal, choanal cleft or/and infraorbital sinus samples were collected from 18 commercial and 31 backyard turkey flocks. The polymerase chain reaction (PCR) technique was performed by using primers specific for detecting the 16S rRNA and vlhA genes of MS. The results showed that 51.61% of backyard and 33.33% of commercial farms were MS-positive. These findings suggested the molecular presence of MS, especially in northern and central regions of Iran. Further, the frequency of MS-positive samples was significantly lower in commercial farms than backyard farms (P<0.05). | ||
| کلیدواژه‌ها | ||
| Mycoplasma synoviae؛ Commercial؛ Backyard؛ Turkey؛ molecular detection؛ Iran | ||
| عنوان مقاله [English] | ||
| تشخیص مولکولی مایکوپلاسما سینوویه بوقلمون های تجاری و سنتی برخی از نقاط ایران | ||
| نویسندگان [English] | ||
| سعید رسولی نژاد1؛ محمد حسن بزرگمهری فرد1؛ حسین حسینی2؛ نریمان شیخی1؛ سعید چرخکار1 | ||
| 1گروه بیماری های طیور و مامایی، دانشکده دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 2گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد کرج، دانشگاه آزاد اسلامی، البرز، ایران | ||
| چکیده [English] | ||
| مایکوپلاسما سینوویه عامل بیماریزای مهم از لحاظ اقتصادی و مسبب التهاب کیسههای هوایی و التهاب مفصلی بوقلمونها می باشد. عفونت ممکن است بدون علامت باشد، اما این عفونت بدون علامت می تواند پرنده را به آلودگی های ثانویه حساس کند. این مطالعه به منظور شناسایی مولکولی مایکوپلاسما سینوویه از مزارع پرورش بوقلمون صنعتی و سنتی ایران شامل استان های تهران، سمنان، اصفهان، قزوین، زنجان، آذربایجان شرقی، گیلان، مازندران و گلستان انجام شد. از 18 مزرعه صنعتی و 31 مزرعه سنتی 600 سواب از نای، شکاف کامی و سینوس دور چشمی پرندگان جدا شد. آزمایش واکنش زنجیرهای پلیمراز با استفاده از پرایمرهای اختصاصی مایکوپلاسما سینوویه به منظور ردیابی ژن های 16S rRNA و vlhA انجام شد. نتایج با هم مقایسه شد و نشان داد که که 51% گله های سنتی و 33.33% گله های صنعتی از لحاظ مایکوپلاسما سینوویه مثبت بودند. بر اساس این نتایج آلودگی مزارع صنعتی و سنتی مایکوپلاسما سینوویه به ویژه با فراوانی بیشتر در استان های شمالی و مرکزی ایران شناسایی شد. همچنین تعداد نمونه های مثبت مایکوپلاسما سینوویه در مزارع صنعتی به میزان معناداری از مزارع سنتی کمتر بود (p<0.05). | ||
| کلیدواژه‌ها [English] | ||
| مایکوپلاسما, تجاری, سنتی, بوقلمون, شناسایی, ایران | ||
| مراجع | ||
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