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پاسخ بیوشیمیایی و عملکردی نعناع فلفلی (Mentha piperita L.) به محلول‌پاشی سالیسیلیک اسید در شرایط تغذیه نیتروژنی | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 12، دوره 34، شماره 2 - شماره پیاپی 88، خرداد و تیر 1397، صفحه 309-329 اصل مقاله (412.86 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2018.115896.2167 | ||
| نویسندگان | ||
| عادل پشت دار* 1؛ علیرضا ابدالی مشهدی2؛ فواد مرادی3؛ سید عطااله سیادت4؛ عبدالمهدی بخشنده4 | ||
| 1دکترای تخصصی، گروه زراعت و اصلاح نباتات، دانشگاه کشاورزی و منابع طبیعی خوزستان، اهواز، ایران | ||
| 2دانشیار، گروه زراعت و اصلاح نباتات، دانشگاه کشاورزی و منابع طبیعی خوزستان، اهواز، ایران | ||
| 3استادیار، گروه فیزیولوژی مولکولی پژوهشکده بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| 4استاد، گروه زراعت و اصلاح نباتات، دانشگاه کشاورزی و منابع طبیعی خوزستان، اهواز، ایران | ||
| چکیده | ||
| روشهای مختلفی به‌منظور افزایش تولید متابولیتهای ثانوی در گیاهان دارویی استفاده میشود، ازجمله آنها افزودن الیسیتورها به گیاهان است. سالیسیلیک اسید اثر مثبتی در رشد و عملکرد گیاهان با اثر بر جذب عناصر معدنی دارد. برای بررسی پاسخ نعناع فلفلی (Mentha piperita L.) به کاربرد سالیسیلیک اسید در سطوح مختلف نیتروژن، آزمایش زراعی دوساله، در سالهای 93-1392 و 94-1393 اجرا گردید. آزمایش به‌صورت اسپلیت پلات بر پایه طرح بلوکهای کامل تصادفی در سه تکرار اجرا شد. تیمارها شامل مقدار کود (0، 70، 140، 210 و 280 کیلوگرم نیتروژن در هکتار) به‌عنوان عامل اول و غلظت سالیسیلیک اسید (0، 100، 200 و 300 میکرومولار) به‌عنوان عامل فرعی بود. نتایج نشان داد که در دو سال، بیشترین میزان فعالیت نیترات ردوکتاز در ریشه با کاربرد غلظت 200 میکرومولار سالیسیلیک اسید و مصرف 280 کیلوگرم نیتروژن در هکتار مشاهده شد. فعالیت این آنزیم در برگ تنها تحت تأثیر نیتروژن قرار گرفت. بیشترین میزان فعالیت آنزیم در برگ (در سال اول و دوم به‌ترتیب 2.87 و 2.82 میکرومولار نیتریت در گرم وزن تازه بر ساعت) با مصرف 280 کیلوگرم نیتروژن در هکتار مشاهده شد. در هر دو سال محتوی نیتروژن و اسید آمینه آزاد در برگ با افزایش غلظت سالیسیلیک اسید، همزمان با افزایش مقدار کود افزایش یافت. هر دو سال محتوای نیترات، فنول کل و کربوهیدرات محلول برگ با افزایش غلظت سالیسیلیک اسید و مقدار کود کاهش یافت. در سال اول و دوم بیشترین عملکرد تر (به ترتیب 3316.2 و 3480.7 گرم در مترمربع)، خشک (به ترتیب 811.7 و 855.6 گرم در مترمربع) و اسانس (به‌ترتیب 13.01 و 15.2 میلیلیتر در مترمربع) با مصرف 210 کیلوگرم نیتروژن در هکتار بدست آمد. در مجموع مصرف 210 تا 280 کیلوگرم در هکتار نیتروژن همراه با کاربرد غلظتهای 200 میکرومولار سالیسیلیک اسید برای دستیابی به عملکرد کمّی و کیفی مطلوب توصیه می شود. | ||
| کلیدواژه‌ها | ||
| اسانس؛ اسید آمینه آزاد؛ ریشه؛ فنول؛ نیترات ردوکتاز | ||
| عنوان مقاله [English] | ||
| Effects of salicylic acid and nitrogen application on biochemistry, qualitative and quantitative yield of peppermint (Mentha piperita L.) | ||
| نویسندگان [English] | ||
| A. Poshtdar1؛ A.R. Abdali Mashhadi2؛ F. Moradi3؛ S.A. Siadat4؛ A. Bakhshandeh4 | ||
| 1Ph.D. of Agronomy, Department of Agronomy, Ramin Agriculture and Natural Resources University of Khouzestan, Ahvaz, Iran | ||
| 2Department of Agronomy, Ramin Agriculture and Natural Resources University of Khouzestan, Ahvaz, Iran | ||
| 3Agricultural Biotechnology Research Institute of Iran, Karaj Branch, Education and Extension Organization (AREEO), Karaj, Iran | ||
| 4Department of Agronomy, Ramin Agriculture and Natural Resources University of Khouzestan, Ahvaz, Iran | ||
| چکیده [English] | ||
| A range of methods are applied to enhance secondary metabolism in medicinal plants. Treatment of the plants with elicitors is one of these approaches. Salicylic acid (SA) exerts a positive impact on plant growth and yield. The present study, as a biannual field experiment, investigates the response of peppermint (Mentha piperita L.) to different rates of SA under different regimes of nitrogen nutrition during years of 2014-2015. The experiment was made as a random complete block design based on split-plot with three replicates. The treatments included nitrogen fertilization rate as the main factor (0, 70, 140, 210, and 280 kg N ha-1 applied as urea) and salicylic acid, as the sub factor, was spayed at different concentrations (0, 100, 200, and 300 μM). In both years, the highest nitrate reductase activity was found in the roots of the plants treated with 200 μM SA and fertilized with 280 kg N ha-1. Interestingly, the foliar activity of the enzyme was influenced only by nitrogen, whereas the highest rate of activity was determined using 280 kg N ha-1 (2.87 and 2.82 μM nitrite g-1 FW h-1, respectively in the first and second year). Higher concentration of SA caused to increased content of nitrogen and free amino acid in the plants fertilized with higher rates of nitrogen. Nitrate, total phenol, and soluble carbohydrates content of leaf was reduced in both years as the result of high rate of nitrogen and high concentration of SA applied. In the first and the second year, the highest fresh yields (3316.2 and 3480.7 g m-2, respectively), the highest dry yields (811.7 and 855.6 g m-2, respectively) and essence (13.01 and 15.2 mL m-2, respectively) were obtained through annual application of 210 kg N ha-1. Collectively, the application of 210 kg N ha-1 together with SA (200μM) is recommended to achieve desired quantitative and qualitative yield. | ||
| کلیدواژه‌ها [English] | ||
| essential oil, free amino acids, root, phenol, nitrate reductase | ||
| مراجع | ||
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- Abdou, M. and Mohamed, M.A.H., 2014. Effect of plant compost, salicylic and ascorbic acids on Mentha piperita L. plants. Biological Agriculture and Horticulture, 30(2): 131-143. - Bagherifard, A., Bagheri, A., Sabourifard, H., Bagherifard, G. and Najar, M., 2015. The effect of salicylic acid on some morphological and biochemistry parameters under salt stress in herb artichoke (Cynara scolymus L.). Research Journal of Fisheries and Hydrobiology, 10(10): 745-750. - Balakrishnan, A., 2015. Therapeutic uses of peppermint-a review. Journal of Pharmaceutical Science and Research, 7(7): 474-476. - Barker, A.V. and Pilbeam, D.J., 2006. Handbook of Plant Nutrition. CRC Press, 196p. - Bideshki, A. and Arvin, M.J., 2010. Effect of salicylic acid (SA) and drought stress on growth, bulb yield and allicin content of garlic (Allium sativum) in field. Plant Ecophysiology, 2: 73-79. - Brown, B., 2003. Mint soil fertility research in the PNW. West Nutrient Management, 5(3): 54-60. - Campbell, W.H., 1999. Nitrate reductase structure, function and regulation: bridging the Gap between biochemistry and physiology. Annual Review Plant Physiology Plant Molecular Biology, 50: 277-303. - Cataldo, D.A., Schrader, L.E. and Youngs, V.L., 1975. Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Communications in Soil Science and Plant Analysis, 6: 71-80. - Croteau, R.B., Ringer, K.L., Davis, E.M. and Wildung, M.R., 2005. (-)-Menthol biosynthesis and molecular genetics. Naturwissen Schaften, 92: 562-577. - Dubios, M.K.A., Gilles, J.K., Hamilton, P.A., Rebers, A. and Smith, F., 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28: 350-356. - Esmaeilzadeh Bahabadi, S. and Sharifi, M., 2013. Increasing the production of plant secondary metabolites using biotic elicitors. Journal of Cell & Tissue, 4(2): 119-128. - Farzana, M.U.Z.N., Tharique, I. and Al-sultana, A., 2014. A review of ethnomedicine, phytochemical and pharmacological activities of Acacia nilotica wild. Journal of Pharmacognosy and Phytochemistry, 3(1): 84-90. - Fedorova, E., Greenwood, J. and Oaks, S.A., 1994. In-situ localization of nitrate reductase in maize roots. Planta, 194: 279-286. - Figueroa Perez, M.G., Rocha-Guzman, N.E., Mercado-Silva, E., Loarca-Pina, G. and Reynoso-Camacho, R., 2014. Effect of chemical elicitors on peppermint (Mentha piperita) plants and their impact on the metabolite profile and antioxidant capacity of resulting infusions. Food Chemistry, 156: 273-278. - Gershenzon, J., McConkey, M.E. and Croteau, R.B., 2000. Regulation of monoterpene accumulation in leaves of peppermint. Plant Physiology, 122: 205-213. - Gharib, F.A., 2007. Effect of salicylic acid on the growth, metabolic activities and oil content of basil and marjoram. International Journal of Agriculture and Biology, 9: 294-301. - Ghasemzadeh, A. and Jaafar, H., 2012. Effect of salicylic acid application on biochemical changes in ginger (Zingiber officinale Roscoe). Journal of Medicinal Plants Research, 6: 790-795. - Hayat, S., Ali, B. and Ahmad, A., 2007. Salicylic acid: biosynthesis, metabolism and physiological role in plants. A Plant Hormone, 1-14. - Hayat, Q., Hayat, S., Alyemeni, M. and Ahmad, N., 2012. Salicylic acid mediated changes in growth, photosynthesis, nitrogen metabolism and antioxidant defense system in Cicer arietinum L. Plant Soil Environment, 58(9): 417-423. - Heobald, J.C., Mitchell, R.A.C., Parry, M.A.J. and Lawlor, D.W., 1998. Estimating the excess investment in ribulose-1,5-bisphosphate carboxylase/oxygenase in leaves of spring wheat grown under elevated CO2. Plant Physiology, 118: 945-955. - Idrees, M., Khan, M.M.A., Aftab, T., Naeem, M. and Hashmi, N., 2010. Salicylic acid-induced physiological and biochemical changes in lemongrass varieties under water stress. Journal of Plant Interaction, 5: 293-303. - Izadi, Z., Ahmadvand, G., Asna Ashri, M. and Piri, J., 2010. Effect of nitrogen and plant density on some growth characteristic, yield and essence in peppermint. Iranian Journal of Field Crops Research, 41: 824-836. - Jain, A. and Srivastava, H.S., 1981. Effect of salicylic acid on nitrate reductase activity in maize seedlings. Physiology of Plant, 51: 339-342. - Jaworski, E.G., 1971. Nitrate reductase assay in intact plant tissues. Biochemical and Biophysical Research Communications, 43(6): 1274-1279. - Khodary, S.E.A., 2004. Effect of salicylic acid on the growth, photosynthesis and carbohydrate metabolism in salt stressed maize plant. International Journal of Biology, 6: 5-8. - Kjeldahl, J., 1883. "Neue Methode zur Bestimmung des Stickstoffs in organischen Körpern" (New method for the determination of nitrogen in organic substances). Zeitschrift für Analytische Chemie, 22(1): 366-383. - Lea, P.J. and Miflin, B.J., 1980. Transport and metabolism of asparagine and other nitrogen compounds within the plants: 567-607. In: Miflin, B.J., (Ed.). The Biochemistry of Plants (Vol. 5). Academic Press, New York, 670p. - Malakouti, M.J., 2004. Fertilizer Use by Crops in Iran. Report Prepared for FAO. Soil and Water Research Institute, Tehran, Iran. - Marotti, M., Piccaglia, R., Crout, W., Craufutd, K. and Deans, S., 2004. Effect of planting time and mineral fertilization on peppermint (Mentha piperita L.) essential oil composition and its biological activity. Flavor and Fragrance Journal, 9(3): 125-129. - Marschner, H., 1995. Mineral Nutrition of Higher Plants. Academic Press, 889p. - Maizura, M., Aminah, A. and Wan Aida, W.M., 2011. Total phenolic content and antioxidant activity of kesum (Polygonum minus), ginger (Zingiber officinale) and turmeric (Curcuma longa) extract. International Food Research Journal, 18(2): 529-534. - Matsuo, T., Kumazawa, K., Ishii, R. Ishihara, K. and Hirata, H., 1995. Science of the Rice Plant. Vol. 2. Physiology. Food and Agricultural Policy Research Center, Tokyo, 248p. - Mir-Hosseini, M., 2007. Effective extraction methods and devices, pharmaceutical and food industries, Zahedan Branch, Islamic Azad University Press, 170p. - Minotti, P.L., Williams, D.C. and Jackson, W.A., 1969. Nitrate uptake by wheat as Influence by ammonium and other cations. Crop Science, 9: 9-14. - Mohammadi Farsani, M. and Ghasemi Pirbalouti, A., 2014. Using elicitors for enhanced production of secondary metabolites in plant cell and organ suspension cultures. Journal of Herbal Drugs (An International Journal on Medicinal Herbs), 5(3): 113-120. - Nguyen, P.M. and Niemeyer, E.D., 2008. Effects of nitrogen fertilization on the phenolic composition and antioxidant properties of basil (Ocimum basilicum L.). Journal of Agricultural and Food Chemistry, 56: 8685-8691. - Omidbaigi, R., 2009. Production and Processing of Medicinal Plants (Vol. 2). Astan Quds Razavi, 438p. - Padash, A., Ghanbari, A. and Asgharipour, M.R., 2016. Effect of salicylic acid on concentration of nutrients, protein and antioxidant enzymes of basil under lead stress. Iranian Journal of Plant Biology, 8(27): 17-32. - Popova, L., Ananieva, V., Hristova, V., Christov, K., Geovgieva, K., Alexieva, V. and Stoinova, Z., 2003. Salicylic acid and methyl jasmonate- induced protection on photosynthesis to paraquat oxidative stress. Bulgarian Journal of Plant Physiology (Special Issue), 133-152. - Poshtdar, A., Abdali Mashhadie, A.R., Moradi, F., Siadat. S.A. and Bakhshandeh, A., 2016. Effect of source and rate of nitogen fertilizer on yield and water and nitrogen use efficiency of peppermint (Mentha piperita L.). Iranian Journal of Crop Sciences, 18(1): 14-31. - Raskin, I., 1992. Role of salicylic acid in plants. Annual Review of Plant Physiology and Plant Molecular Biology, 43: 439-463. - Ravindranth, M.H., 1981. Manual Research Method for Crustacean Biochemistry and Physiology. Special Publication, 172p. - Raun, W.R. and Johnson, G.V., 1999. Improving nitrogen use efficiency for cereal production. Agronomy Journal, 91: 357-363. - Singh, V.P., Chatterjee, B.N. and Singh, P., 2003. Response of mint species to nitrogen fertilization. Journal of Agriculture Science, 113(2): 267-271. - Zeinali, H., Hosseini, H. and Shirzadi, M.H., 2014. Effects of nitrogen fertilizer and harvest time on agronomy, essential oil and menthol of Mentha piperita L. Iranian Journal of Medicinal and Aromatic Plants, 30(3): 486-495. - Zhao, J., 2006. The effect of nitrogen fertilization on spearmint. Journal of Essential Oil Research, 18: 452-455. | ||
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