| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شمارهها | 3,094 |
| تعداد مقالات | 34,533 |
| تعداد مشاهده مقاله | 49,384,036 |
| تعداد دریافت فایل اصل مقاله | 79,835,060 |
باززایی گیاه در معرض انقراض لاله واژگون (Fritillaria raddeana) از طریق ریزنمونههای گلبرگ و برگ | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 9، دوره 26، شماره 1 - شماره پیاپی 51، تیر 1397، صفحه 71-83 اصل مقاله (724.87 K) | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2018.116658 | ||
| نویسندگان | ||
| سلاله صلاحی صدر1؛ هدایت زکی زاده* 2؛ محمد رضا نقوی3؛ جمال العلی الفتی4 | ||
| 1دانشجوی دکترای مهندسی علوم باغبانی (گیاهان زینتی)، دانشکده علوم کشاورزی، پردیس دانشگاهی دانشگاه گیلان | ||
| 2نویسنده مسئول مکاتبات، استادیار گروه علوم باغبانی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت. پست الکترونیک:Zakizadeh@guilan.ac.ir | ||
| 3استاد، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران | ||
| 4استادیار گروه علوم باغبانی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت | ||
| چکیده | ||
| لاله واژگون (raddeana Fritillaria)، یکی از منحصر بهفردترین و زیباترین گیاهان خودرو و بومی ایران است. این جنس از خانواده سوسن بهشدت در معرض انقراض قرار دارد. گونه Fritillaria raddeana مقاوم به خشکی، مناطق سنگلاخی و شیبدار میباشد و بهدلیل مطالعات محدود در این گونه، انجام هر گونه پژوهش در این گیاه اهمیت بهسزایی دارد. با وجود احتمال وقوع جهش، باززایی موفقیتآمیز پیشنیاز کلیه مطالعاتی است که باید در گونههای رو به انقراض انجام شود. این پژوهش کالوسزایی و باززایی غیرمستقیم را از کشت اندامهای گلبرگ و برگ F. raddeana در محیط درون شیشهای مورد بررسی قرار داد. ریزنمونههای برگ پیش از گلدهی و ریزنمونههای گلبرگ در حالت غنچه (سبز تا زرد) برداشت شد و پس از ضدعفونی، در محیط کشت موراشیگ و اسکوگ همراه با غلظتهای مختلف از اکسین و سایتوکینین کشت شدند. ریزنمونهها برای القای کالوس ابتدا در تاریکی و دمای C°18 و بعد به روشنایی با شرایط دمایی قبل انتقال یافتند. پس از واکشتِ کالوسها در محیطهای باززایی، دما به C°20 افزایش یافت. اگرچه هر دو نوع ریزنمونه بهدلیل عدم آسیب به گیاهان مادری، درصد نسبتا پایین آلودگی داخلی، تعداد و اندازه مناسب، ریزنمونههای قابل قبولی بودند، با اینحال ریزنمونه گلبرگ دارای پاسخدهی بهتر و سریعتر به انواع ترکیبات تنظیمکننده رشد بود. بهترین محیط کشت کالوسزایی (66/82%) و باززایی غیرمستقیم (66/36%) از ریزنمونههای گلبرگ، محیط MS حاوی 5/0 میلیگرم در لیتر ایندول بوتیریک اسید همراه با 2 میلیگرم در لیتر تیدیازورون تشخیص دادهشد که نشاندهنده برتری این دو نوع تنظیمکننده در باززایی غیرمستقیم F. raddeana بود. | ||
| کلیدواژهها | ||
| باززایی غیرمستقیم؛ تنظیمکننده رشد؛ کالوسزایی؛ گونههای وحشی؛ گیاه بومی | ||
| عنوان مقاله [English] | ||
| Regeneration of an endangered Fritillaria (Fritillaria raddeana) species via petal and leaf explants | ||
| نویسندگان [English] | ||
| S. Salahi Sadr1؛ H. Zakizadeh2؛ M.R. Naghavi3؛ J.A. Olfati4 | ||
| 1Ph.D. Student, Department of Horticultural Sciences, University Campus 2, University of Guilan, Rasht, I.R. Iran. | ||
| 2Corresponding Author, Assist. Prof., Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, I.R. Iran. Email: Zakizadeh@guilan.ac.ir | ||
| 3Prof., Dept. of Agronomy and Plant Breeding, Agricultural & Natural Resources College, University of Tehran, Karaj, I.R. Iran | ||
| 4Assoc. Prof., Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, I.R. Iran | ||
| چکیده [English] | ||
| Fritillaria raddeana is one of the most fascinating wild flowers, belonging to Lilliaceae family, native to Iran. It is an important ornamental-medical bulbous plant, tolerant to arid conditions and stony slopes but facing extinction. There have been very few reports exist, so any studies on the species would be of great importance. Regardless of mutation occurrence, successful regeneration will be a prerequisite for further researches that must be done on the endangered plant species. This research presents an in vitro callus induction and indirect regeneration of F. raddeana via culturing petal and leaf. The leaf (before flowering) and petal (green to yellow flower buds) explants excited from plant and surface sterilized and were cultured on MS media containing different concentrations of Auxin and Cytokinin. The explants were first kept at darkness and 18ºC for callus induction, then were transferred to light condition with the same temperature. The temperature was raised to 20ºC, when the calli sub cultured into regeneration media. Although due to the low amount of bacterial and fungal contamination, conservation of parent plants, adequate and ready available explants, both petals and leaves are acceptable explants, among them petal showed better and quicker response to variable plant growth regulators. The best medium for callus formation (82.66%) and indirect regeneration (36.66%) via petal explants was MS medium supplemented with 2 mgl-1 TDZ and 0.5 mgl-1 IBA. Therefore, the mentioned plant growth regulators were more suitable than the others for indirect regeneration of F. raddeana. | ||
| کلیدواژهها [English] | ||
| Callus formation, endemic plant, indirect regeneration, plant growth regulators, wild species | ||
| مراجع | ||
|
- Aggarwal, D. and Barna, K.S., 2004. Tissue culture propagation of elite plant of Aloe vera Linn. Journal of Plant Biochemistry and Biotechnology, 13(1): 77-79. - Appelgren, M. and Heide, O.M., 1972. Regeneration in Streptocarpus leaf discs and its regulation by temperature and growth substances. Plant Physiology, 27: 417-423. - Arteca, R.N., 2013. Plant Growth Substances: Principles and Applications. Springer Science & Business Media. 332 p. - Badenoch-Jones, J., Parker, C.W., Letham, D.S. and Singh, S., 1996. Effect of cytokinins supplied via the xylem at multiples of endogenous concentrations on transpiration and senescence in derooted seedlings of oat and wheat. Plant, Cell & Environment, 19(5): 504-516. - Bonga J.M., 1987. Tree Tissue Culture Applications. Vol. 5: Advances in Cell Culture. 209-239. In: K. Maramorosch (Ed). Academic Press, New York: .297 p. - Gharehyazie, B., Ebrahimie, E., Mohammadi Deh-cheshmeh, M., Irannegad, A., Sardari, M. and Salahi Ardacany, A., 2006. Development of commercial protocol for in vitro micropropagation of Fritillaria. International System for Agricultural Science and Technology. Record ID=IR2008000622. - Frey, L. and Janick, J., 1991. Organogenesis in carnation. Journal of the American Society for Horticultural Science, 116(6):1108-1112. - Gamborg, O.L., Miller, R. and Ojima, K., 1968. Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50(1): 151-158. - Gao, S.L., Zhu, D.N., Cai, Z.H., Jiang, Y., and Xu, R., 1999. Organ culture of a precious Chinese medicinal plant – Fritillaria unibracteata, Plant Cell, Tissue and Organ Culture, 59: 197–201. - Gholami, M. 2007. Micropropagation of of inverted tulip (Fritillaria imperialis L.). MSc. Thesis, University of Avicenna. Hamedan, Iran (In Persian). - Hackett, W.P., 1969. Aseptic multiplication of lily bulblets from bulb scales. The International Plant Propagators' Society, 19: 105-108. - Hoseinpanahi, S., Majdi, M. and Mirzaghaderi, Gh., 2016. Effects of growth regulators on in vitro callogenesis and regeneration of black cumin (Nigella sativa), Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 24(2): 232-242. - Jevremović, S., Petrić, M., Živković, S., Trifunović, M. and Subotić, A., 2010. Superoxide dismutase activity and isoenzyme profiles in bulbs of snake's head fritillary in response to cold treatment. Archives of Biological Sciences,62(3): 553-558. - Kulkhanova, D.S., Erst, A.A. and Novikova, T.I., 2015. In vitro regeneration from bulbous scales of Fritillaria sonnikovae, an endemic species. Russian Journal of Developmental Biology, 46(4): 215-221. - Kumar, S. and Kanwar, J.K., 2006. Regeneration ability of petiole, leaf and petal explants in gerbera cut flower cultures in vitro. Folia Horticulture, 18(2): 57-64. - Le Nard, M. and De Hertogh. A.A., 1993. The Physiology of Flower Bulbs. Elsevier, Amsterdam, the Netherlands. 812 p. - Liao, Z., Chen, M., Tan, F., Sun, X. and Tang, K., 2004. Microprogagation of endangered Chinese aloe. Plant Cell, Tissue and Organ Culture, 76(1), 83-86. - Mohammadi Deh-cheshmeh, M., Khalighi, A. and Naderi, R., 2007. Indirect somatic embryogenesis from petal expiant of endangered wild population of Fritillaria imperialis. Pakistan Journal of Biological Sciences, 10(11): 1875-1879. - Mohammadi Deh-cheshmeh, M., Khalighi, A., Naderi, R., Sardari, M. and Ebrahimie, E., 2008. Petal: a reliable explant for direct bulblet regeneration of endangered wild populations of Fritillaria imperialis L. Acta Physiologiae Plantarum, 30(3): 395-399. - Muraseva, D.S., Novikova, T.I. and Erst, A.A., 2015. In vitro propagation and conservation of rare species Fritillaria meleagris L. from floral explants.Contemporary Problems of Ecology, 8(6): 754-763. - Murashige, T. and Skoog, F., 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3): 473-497. - Nikolić, M., Radojević, L., Jevremović, S., Trifunović, M. and Subotić, A., 2006. In vitro formation of somatic embryos and bulblets of Fritilllaria meleagris. - In: Plant, Fungal and Habitats Diversity Investigation and Conservation (Book of Abstracts of IV Balkan Botanical Congress). P. 136. - Nowruzian, A., Masoumian, M., Ebrahimi, M.A. and Bakhshi Khaniki, G.R., 2016. Micropropagation of Ferula assa-foetida L.. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 24(1): 121-133. - Nugent, G., Aerdley-Richardson, T., Lu, C.Y., 1991. Plant regeneration from stem and petal of carnation (Dianthus caryophyllus L.). Plant Cell Reports, 10: 477–480. - Palmer, C.D. and Keller, W.A., 2011. Plant regeneration from petal explants of Hypericum perforatum L. Plant Cell, Tissue and Organ Culture, 105(1): 129-134. - Pierik, R.L.M. and Steegmans, H.H.M., 1975. Effect of auxins, cytokinins, gibberellins, abscisic acid and ethephon on regeneration and growth of bulblets on excised bulb scale segments of hyacinth. Plant Physiology, 34: 14-17. - Pintos, B., Martin, J.P., Centeno, M.L., Villalobos, N., Guerra, H., Martin, L., 2002. Endogenous cytokinin levels in embryogenic and nonembryogenic calli of Medicago arborea L. Plant Science, 163:955– 960. - Preece, J. E. and Imel, M. R., 1991. Plant regeneration from leaf explants of Rhododendron 'P. J. M. hybrids. Scientia Horticulturae, 48:159-170. - Rachinger, K.H, 1990. Flora Iranica, vol. 165, Liliaceae, 61-76. - Safarnejad, A., 2015. Effects of growth regulators on in vitro regeneration of Ziziphus jujube. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research. 23(1): 40-48. - Salahi Sadr. S., Zakizadeh, H., Naghavi, M.R., Aryakia, E., 2015. Callus induction from bulb-scales explant of endangered wild species of Fritillaria raddeana. 9th Congress of Iranian Horticultural Science (Book of Abstract of 9th Congress of Iranian Horticultural Science, Shahid Chamran University of Ahwaz). P.389. (In Persian). - Subotić, A., Trifunović, M., Jevremović, S. and Petrić, M., 2010. Morpho-histological study of direct somatic embryogenesis in endangered species Frittilaria meleagris. Biologia Plantarum, 54(3): 592-596. - Sun, C.S. and Wang D.Y.,. 1991. Fritillaria spp. (Fritillary): In Vitro Culture and the Regeneration of Plants. Biotechnology in Agriculture and Forestry, Medicinal and Aromatic Plants III, vol 15: 258–269. - Liu, Y.J., Yin, H. and Zhu, S.Y., 2002. Thidiazuron induced physiological and biochemical changes in the process of dedifferentiation of Fritillaria ussuriensis. Acta Botanica Boreali-Occidentalia Sinica, 23(3): 433-437. - Turk, B.A., Swartz, H.J. and Zimmerman, R.H., 1994. Adventitious shoot regeneration from in vitro-cultured leaves of Rubus genotypes. Plant Cell, Tissue and Organ Culture, 38(1): 11-17. - Witomska, M. and Lukaszewska. A., 1997. Bulbet regeneration in vitro from different explants of Fritillaria imperialis. Acta Horticulturae,430: 331-338. - Wu, X., Li, X. and Zhang, X., 2008. Molecular analysis of hormone regulated petal regeneration in Petunia. Plant Cell Reports, 27: 1169–1176. - Yue-hua, W.A.N.G., Kuojun, Z. and Lijun, Z., 2011. Study on the high efficiency callus induction system by Fritillaria cirrhosa D. Don leaves. Journal of Anhui Agricultural Sciences, 3:47-55. | ||
|
آمار تعداد مشاهده مقاله: 684 تعداد دریافت فایل اصل مقاله: 577 |
||