| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شماره‌ها | 3,086 |
| تعداد مقالات | 34,442 |
| تعداد مشاهده مقاله | 48,918,883 |
| تعداد دریافت فایل اصل مقاله | 79,355,689 |
تاثیر ویروس مخطط توتون بر غلافبندی ارقام مختلف سویا در شرایط گلخانه | ||
| آفات و بیماری های گیاهی | ||
| مقاله 2، دوره 86، شماره 2 - شماره پیاپی 107، اسفند 1397، صفحه 133-145 اصل مقاله (271.63 K) | ||
| نوع مقاله: بیماری‌شناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2018.121190.1216 | ||
| نویسندگان | ||
| سمیرا شاملی1؛ فرشاد رخشنده رو* 2؛ محمد رضا صفرنژاد3؛ سیده ساناز رمضانپور4 | ||
| 1دانشجوی دکتری- گروه گیاهپزشکی، دانشکده علوم کشاورزی وصنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 2گروه گیاهپزشکی، دانشکده علوم کشاورزی وصنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 3بخش تحقیقات ویروس شناسی، موسسه تحقیقات گیاهپزشکی، سازمان تحقیقات، آموزش و ترویج کشاورزی | ||
| 4گروه بیوتکنولوژی و اصلاح نباتات، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران | ||
| چکیده | ||
| ویروس مخطط توتون (Tobacco streak virus) یکی از مهم‌ترین ویروس‌های مخرب محصولات زراعی بوده و به‌عنوان عامل سوختگی جوانه‌های سویا در نقاط مختلف دنیا گزارش شده است. در این پژوهش، واکنش ارقام رایج سویا در شمال کشور نسبت به این ویروس و تأثیر احتمالی آن بر سویا در شرایط گلخانه ارزیابی شد. پژوهش به‌صورت طرح کاملاً تصادفی در قالب فاکتوریل با دو تیمار جدایه‌های ویروس (جدایه‌های سویا، ماش و آفتابگردان) و ارقام سویا (ویلیامز، امیر، کتول، سامان، ساری) در سه تکرار انجام شد. برای اثبات آلودگی بوته‌ها از آزمون الایزای مستقیم با استفاده از آنتی‌سرم چند همسانه‌ای TSV و واکنش زنجیره‌ای پلیمراز با استفاده از آغازگرهای اختصاصی ویروس استفاده شد. نتایج نشان داد جدایه‌های ویروس به‌طور معنی‌داری باعث کاهش ارتفاع بوته‌ها، کاهش تعداد گره‌ها و کاهش تعداد غلاف‌های سالم شدند. ارقام نیز دارای تأثیرمعنی‌دار برشاخص‌های رشدی ارتفاع بوته ، تعداد گره، تعداد غلاف‌سالم، و وزن تر بوته‌ها بودند. کمترین میزان تفاوت شاخص‌های رشدی و میزان غلافبندی نسبت به نمونه شاهد در رقم ویلیامز و بیشترین آنها در ارقام امیر و کتول مشاهده گردید. شدت علایم ایجاد شده توسط جدایه سویا بیشتر از دو جدایه ماش و آفتابگردان بود. نتایج حاکی از تأثیر معنی‌دار جدایه‌های ویروس و ارقام بر غلافبندی سویا بود. | ||
| کلیدواژه‌ها | ||
| غلافبندی سویا؛ گلخانه؛ ویروس مخطط توتون (TSV) | ||
| عنوان مقاله [English] | ||
| Effect of Tobacco streak virus on in different soybean cultivars in greenhouse conditions | ||
| نویسندگان [English] | ||
| Samira Shameli1؛ Farshad Rakhshandehroo2؛ Mohammadreza Safarnejad3؛ Seyedeh Sanaz Ramezanpoor4 | ||
| 1Department of Plant Protection, College of Agriculture Sciences and Food Industries , Science and Research Branch, Islamic Azad university, Tehran, Iran | ||
| 2Department of Plant Protection, College of Agriculture Sciences and Food Industries , Science and Research Branch, Islamic Azad university, Tehran, Iran | ||
| 3Department of Plant virology, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran | ||
| 4Faculty of plant production,Gorgan University of Agricultrul Sceince and Natural Resources, Gogan, Iran | ||
| چکیده [English] | ||
| Tobacco streak virus is one of the most important destructive viruse of crops, and reported as a causal agent of soybean bud blight in different region of world. In this study, the response of soybean cultivars, that were common in the north of Iran, was evaluated to TSV and the possible effect of virus on soybean, under greenhouse conditions. This research was carried out in two way factorial arrangement on a CRD (completely randomized design) with virus isolates (soybean, mungbean and sunflower isolates) and soybean cultivars (Williams, Amir, Katol, Saman, Sari) as treatments in 3 replications. Plants were tested for TSV infection by DAS-ELISA method with specific polyclonal antibody, and polymerase chain reaction (PCR) with specific designed primer. Results showed that viral isolates significantly reduced the plant length, number of nodes, and number of healthy pods. Cultivars had significant effect on growth indices: plant length, number of nodes, number of pod and fresh weight, too. The lowest differences in growth indices and podding in compare of control group observed in Williams cultivar whereas the highest seen in Amir and Katol cultivars. The symptoms caused by soybean isolate were more severe than mungbean and sunflower isolates. Results indicated significant effect of viral isolates and soybean cultivars on podding. | ||
| کلیدواژه‌ها [English] | ||
| Greenhouse, podding, soybean, Tobacco streak virus (TSV) | ||
| مراجع | ||
|
, G. N. 2005. Plant pathology, 5th ed. Elsevier Academic Press, San Diego, 105 p. AHMED, W., T. B. BUTT, J. IHSAN and A. REHMAN, 2003. Natural occurrence of Tobacco Streak Virus in cotton in Pakistan and screening for its resistant sources. Pakistan Journal of Botany, 35: 401-408. ALMEIDA, A. M. R., A. BERGAMIN FILHO and L. AMORIM, 1994. Disease progress of soybean bud blight and spatial pattern of diseased plants. Journal of Plant Disease and Protection, 101: 386-392. ALMEIDA, A. M. R., S. J. AKAI, K. HANADA, T. G. OLIVEIRA, B. P. ELINTANI, E. W. KITAJIMA, E. R. SOUTO, T. G. NOVAES and P. S. NORA, 2005. Biological and molecular characterization of an isolate of Tobacco Streak Virus obtained from soybeans in Brazil. Fitopatologia Brasileira, 30: 366-373. ARUN KUMAR, N., M. LAKSHMINARASU, U. B. ZEHR and K. S. RAVI, 2006. Natural occurrence and distribution of Tobacco streak virus in south India. Indian Journal of Plant Protection, 34: 54-58. ARUN KUMAR, N., M. LAKSHMINARASU, U. B. ZEHR and K. S. RAVI, 2008. Molecular characterization of Tobacco streak virus causing soybean necrosis in India. Indian Journal of Biotechnology, 7: 214-217. AZIZI, A. and M. SHAMSBAKHSH, 2014 .Impact of cucumber mosaic virus infection on the varietal traits of common bean cultivars in Iran.Virus Diseases, 25: 447–454 BHASKARA REDDY, B. V., Y. SIVAPRASAD, C. V. M. NARESH KUMAR, A. SUJITHA, K. RAJA REDDY and DV. SAI GOPAL, 2012. First Report of Tobacco Streak Virus infecting kenaf (Hibiscus cannabinus) in India. Indian Journal of Virology, 23(1): 80-82. BHAT, A. I., R. K. JAIN and M. RAMIAH, 2002a. Detection of Tobacco Streak Virus from sunflower and other crops by reverse transcription polymerase chain reaction. Indian Phytopathology, 55: 216-218. BHAT, A. I., R. K. JAIN, A. VERMA and S. K. LAL, 2002b. Nucleocapsid protein gene sequence studies suggest that soybean bud blight is caused by a strain of Groundnut bud necrosis virus. Current Science, 82: 1389-1392. CHERAGHALI, A. 1990. Final reports of the comprehensive project on Podding Disorder of Soybean. Agricultural and Natural Resources Research Center of Golestan (In Persian). CLARK, M. F. and S. A. N. ADAMS, 1977. Characteristics of micro plates method of enzyme-linked-immunosorbent assay for detection of plant viruses. Journal of General Virology, 34: 475-483. COSTA, A. S. and A. M. B. CARVALHO, 1961. Studies on Brazilian Tobacco Streak Virus. Phytopathology. Zeitsch, 42: 113-138 GHANEKAR, A. M. and F. W. SCHWENK, 1974. Seed transmission of Tobacco streak virus in six cultivars of soybeans. Phytopathology, 64:112–114. GOLNARAGHI, A. R., N. SHAHRAEEN, R. POURRAHIM, S. FARZADFAR and A. GHASEMI, 2004. Occurrence and relative incidence of viruses infecting soybeans in Iran. Plant Disease, 88: 1069-1074. HILL, C. B., C. R. BOWEN and G. L. HARTMAN, 2013. Effect of fungicide application and cultivar on soybean green stem disorder. Plant Disease, 97: 1212-1220. HILL, C. B., G. L. HARTMAN, R. ESGAR and H. A. HOBBS, 2006. Field evaluation of green stem disorder in soybean cultivars. Crop Science, 46: 879-885. HOBBS, H. A., C. B. HILL, C. R. GRAU, N. C. KOVAL, Y. WANG, L. PEDERSEN, L. L. DOMIER and G. L. HARTMAN, 2006. Green stem disorder of soybean. Plant Disease, 90: 513-518. HOBBS, H. A., S. JOSSEY, Y. WANG, G. L. HARTMAN and L. L. DOMIE, 2012. Diverse soybean accessions identified with temperature-sensitive resistance to Tobacco Streak Virus. Crop Science, 52: 738-744. HOSSEINI, S., M. KOOHI HABIBI, G. MOSAHEBI, M. MOTAMEDI and S. WINTER, 2012. First report on the occurrence of Tobacco Streak Virus in sunflower in Iran. Journal of Plant Pathology, 94: 585-589. IRIZARRY, M. D., C. L. GROVES, M. G. ELMORE, C. A. BRADLEY, R. DASGUPTA, T. L. GERMAN, D. J. JARDINE, E. S. SAALAU ROJAS, D. L. SMITH A. U. TENUTA, S. A. WHITHAM and D. S. MUELLER, 2016. Re-emergence of Tobacco streak virus infecting soybean in the United States and Canada. Plant Health Progress, 17: 92-94. JAGTAP, G. P., T. H. JADHAV and D. UTPAL, 2012. Occurrence, distribution and survey of Tobacco Streak Virus (TSV) of cotton. Scientific Journal of Crop Science, 1: 16-19. JAIN, R. K., S. BAG and L. P. AWASTHI, 2005.First report of natural infection of Capsicum annuum by Tobacco streak virus in India. Plant Pathology, 54: 257. KAISER, W. J., S. D. WYATT and G. R. PESHO, 1982. Natural hosts and vectors of Tobacco Streak Virus KALYANI, G., A. S. REDDY, P. L. KUMAR, D. V. J. P. RAO, R. ARUNA, F. WALIYAR and S. NIGAM, 2007. Sources of resistance to Tobacco Streak Virus in wild Arachis (Fabaceae: Papilionoidae) germplasm. Plant Diseases, 91:1585-1590. KAZINCZI, G., D. LUKAS, A. TAKACS, J. HORVATH, R. GABORJANYI, M. NADASY and E. NADASY, 2006. Biological decline of Solanum nigrum due to virus infections. Journal of Plant Diseases and Protection, 20: 325-330. KOLLMANN, J., M. J. BANUELOS and S. L. NIELSEN, 2007. Effects of virus infection on growth of the invasive alien Impatiens glandulifera. Preslia, 79: 33-44. KUMAR, P. L., T. K. S. LATHA, N. K. KULKARNI, K. RAGHAVENDRA, K. B. SAXENA, F. WALIYAR, K. T. RANGASWAMY, V. MUNIYAPPA, S. DORISWAY and A. T. JONES, 2005. Broad-based resistance to pigeonpea sterility mosaic disease in wild relatives of pigeonpea (Cajanus: Phaseoleae). Annuals of Applied Biology, 146: 371-379. POORTER, H. and R. DE JONG, 1999. A comparison of specific leaf area, chemical composition and leaf construction costs of field plants from 15 habitats differing in productivity. New Phytologist, 143: 163-176. PRASAD RAO, R. D. V. J., A. S. REDDY, R. S. CHANDER, K. S. VARAPRASAD and D. K. THIRUMALA, 2000. Tobacco Streak Ilarvirus as causal agent of sunflower necrosis disease in India. Journal of Oilseeds Research, 17: 400-401. PRASAD RAO, R. D. V. J., A. S. REDDY, S. V. REDDY, K. THIRUMALADEVI, S. CHANDER RAO, V. MANOJ KUMAR, K. SUBRAMANYAM, T. YELLAMANDA REDDY, S. N. NIGAM and D. V. R. REDDY, 2003. The host range of Tobacco streak virus in India and transmission by thrips. Annuals of Applied Biology, 142: 365-368. RABEDEAUX, P. F., J. M. GASKA, N. C. KURTZWEIL and C. R. GRAU, 2005. Seasonal progression and agronomic impact of Tobacco Streak Virus on soybean in Wisconsin. Plant Diseases, 89: 391-396. RAHIMIAN, H., A. HAMDOLAHZADEH and M. MONTAZERI, 1996. Study of implication of prokaryote, virous and viroid organisms in Soybean podding disorder. The 12th Iranian Plant Pathology Congress (In Persian). RANDEY, S. P. and I. E. SOMSSICH, 2009. The role of WRKY transcription factors in plant immunity. Plant Physiology, 150: 1648–1655. RAVI, K. S., A. BUTTEGEREITT, A. S. KITKARU, S. DESHMUKH, D. E. LESEMANN and S. WINTER, 2001. Sunflower necrosis disease from India is caused by an Ilarvirus related to Tobacco Streak Virus. Plant Pathology,50: 800. REDDY, A. S., L. J. REDDY, N. MALLIKARJUNA, M. D. ABDURAHMAN, Y. V. REDDY, P. J. BRAMEL, and V. D. R. REDDY, 2000. Identification of resistance to Peanut bud necrosis virus in wild Arachis germplasm. Annuals of Applied Biology, 137:135-139. ROSS, J. P. 1986. Registration of four soybean lines resistant to bean pod mottle virus. Crop Science, 26:210. SHARMAN, M., J. E. THOMAS and D. M. PERSLEY, 2008. First report of Tobacco Streak Virus in sunflower (Helianthus annuus), cotton (Gossypium hirsutum), chickpea (Cicer arietinum) and mung bean (Vigna radiata) in Australia. Plant Disease, 3: 27-29. SHARMAN. M., D. E. PAGENDAM, D. M. PARSLEY, A. DRENTH and J. E. THOMAS, 2016. Field evaluation of tolerance to Tobacco streak virus in sunflower germplasm, and observations of seasonal disease spread. Annals of Applied Biology, 168: 390-399 SIVAPRASAD, Y., B. V. BHASKARA REDDY, K. REKHA RANI, K. RAJA REDDY and D. V. R. SAI GOPAL, 2010. First report of Tobacco Streak Ilarvirus infecting onion (Allium cepa). New Diseases, 22: 17. VINODKUMAR, S., S. NAKKEERAN, V. G. MALATHI, G. KARTHIKEYAN, P. AMALA BALU, S. MOHANKUMAR and P. RENUKADEVI, 2017. Tobacco streak virus: an emerging threat to cotton cultivation in India. Phytoparasitica, 45: 729-743. WANG, Y., H. A. HOBBS, C. B. HILL, L. L. DOMIER, G. L. HARTMAN and R. L. NELSON, 2005. Evaluation of ancestral lines of U.S. soybean cultivars for resistance to four soybean viruses. Crop Science, 45: 639–644. | ||
|
آمار تعداد مشاهده مقاله: 1,033 تعداد دریافت فایل اصل مقاله: 823 |
||