| تعداد نشریات | 284 |
| تعداد نشریات سازمان | 82 |
| تعداد شماره‌ها | 3,037 |
| تعداد مقالات | 33,984 |
| تعداد مشاهده مقاله | 45,785,062 |
| تعداد دریافت فایل اصل مقاله | 77,193,184 |
سنتز نانو ذرات بر پایه سورفکتنت حاوی اسانس Mentha piperita L. و Mentha pulegium L. به‌منظور بررسی رهایش اسانس و فعالیت آنتی‌اکسیدانی | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 2، دوره 34، شماره 3 - شماره پیاپی 89، مرداد و شهریور 1397، صفحه 359-379 اصل مقاله (793.18 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2018.110161.1997 | ||
| نویسندگان | ||
| مطهره السادات مظلوم1؛ سمیرا نادری نژاد* 2؛ فرشته واحدیان1؛ فاطمه حقیرالسادات3؛ فاطمه دانشمند4 | ||
| 1دانشجوی کارشناسی ارشد، گروه بیوشیمی، دانشگاه پیام نور تفت، یزد، ایران | ||
| 2کارشناس‌ارشد مهندسی بیوتکنولوژی، گروه مهندسی بیوتکنولوژی و داروسازی، دانشکده مهندسی شیمی، پردیس دانشکده‌های فنی، دانشگاه تهران، تهران، ایران | ||
| 3دکترای نانوبیوتکنولوژی، گروه مهندسی علوم زیستی، دانشکده علوم و فنون نوین، دانشگاه تهران، تهران، ایران | ||
| 4استادیار، گروه زیست‌شناسی، دانشگاه پیام نور، یزد، ایران | ||
| چکیده | ||
| اسانس‌ها ترکیب‌هایی بسیار فرّار هستند. انکپسوله کردن اسانس درون حامل دارویی باعث کاهش فرّاریت اسانس، کاهش اکسیدشوندگی و افزایش بازدهی اثرگذاری اسانس می‌شود. هدف از این تحقیق ساخت نانونیوزوم زیست سازگار حاوی اسانس نعناع (Mentha piperita L.) و پونه (Mentha pulegium L.) و توسعه سامانه گیاهی-دارویی با میزان بارگذاری مناسب می‌باشد. از این‌رو، اثر مقدار و نوع سورفکتنت و همچنین مقدار فسفولیپید بر روی فرمولاسیون‌های مختلف نیوزومی بررسی شد. در ادامه با بررسی اثر اسیدیته بافر هیدراته کننده در pHهای 4/7 (خنثی)، 5 (اسیدی) و 9 (بازی) فرمولاسیون بهینه از نظر درصد بارگذاری اسانس معرفی شد. همچنین سینتیک رهایش اسانس در بافر PBS و دو pH، 7.4 (سلول سالم) و 4/5 (سلول سرطانی) و همچنین در دو سطح دماهای 37 (شرایط فیزیولوژیک) و 42 (سلول سرطانی) درجه سلسیوس بر رهایش سامانه حاوی اسانس بررسی شد. فرمولاسیون‌های مختلف نیوزومی با استفاده از روش هیدراتاسیون فیلم نازک تهیه شد و اندازه ذرات، مورفولوژی، پتانسیل زتا و برهم‌کنش شیمیایی مشخصه‌یابی شد و بعد خاصیت آنتی‌اکسیدانی نانوذرات با روش مهار رادیکال DPPH ارزیابی شد. نتایج این تحقیق نشان داد که فرمولاسیون نیوزومی حاوی فسفاتیدیل کولین سویا، کلسترول و توئین 60 بارگذاری شده با اسانس نعناع یا پونه با غلظت mg/mL5/0، هیدراته شده با PBS در 9=pH بیشترین بازدهی بارگذاری را نشان داده‌است. میزان اسانس بارگذاری شده برای اسانس نعناع و پونه به‌ترتیب 61.36% و 74.31% می‌باشد. ذرات به‌صورت کروی با اندازه کمتر از 80 نانومتر و آنیونی می‌باشند. همچنین میزان رهایش اسانس در شرایط اسیدی و دمای 42 درجه سلسیوس بیشتر از شرایط فیزیولوژیک می‌باشد که نشان می‌دهد فرمولاسیون تهیه شده حساس به دما و pH می‌باشد. نتایج حاصل از آنالیز طیف مادون قرمز نشان داده است که بین اسانس و نیوزوم برهم‌کنش شیمیایی رخ نداده است و بارگذاری اسانس درون نیوزوم‌ها به‌صورت فیزیکی بوده است. همچنین خاصیت آنتی‌اکسیدانی اسانس طی درون‌گیری حفظ شده‌است. | ||
| کلیدواژه‌ها | ||
| نیوزوم؛ نعناعیان؛ فعالیت آنتی‌اکسیدانی؛ آهسته رهش؛ سرطان؛ حساس به دما و .pH | ||
| عنوان مقاله [English] | ||
| Synthesize of nanoparticle-based surfactants containing Mentha piperita L. and Mentha pulegium L. essential oil in order to study the essential oil release and antioxidant activity | ||
| نویسندگان [English] | ||
| M. Mazloom1؛ S. Naderinezhad2؛ F. Vahedian1؛ F. Haghiralsadat3؛ F. Daneshmand4 | ||
| 1M.Sc. student, Department of Biochemistry, Payame Noor University, Taft, Yazd, Iran | ||
| 2M.Sc Biotechnology, Department of Biotechnology and Pharmaceutical Engineering, School of Engineering, University of Tehran, Tehran, Iran | ||
| 3Ph.D. in Nanobiotechnology, Faculty of New Science and Technologies, University of Tehran, Tehran, Iran | ||
| 4Department of Biology, Payam Nour University, Taft, Yazd, Iran | ||
| چکیده [English] | ||
| Essential Oils (EO) are highly volatile compounds. Encapsulation of EO into the nano-carrier leads to reduced EO volatility and oxidation, as well as increased therapeutic efficiency. The aim of this study was to synthesize a biocompatible nano-niosome containing Mentha piperita L. and Mentha pulegium L. EO and develop herbal-medicine system with suitable encapsulation efficiency. Hence, the amount and type of surfactant and also the amount of phospholipid were investigated in various niosomal formulations. Furthermore, the optimized formulation was introduced in terms of EO loading efficiency by investigating the acidity effect of hydrating buffer at 7.4 (neutral), 5.0 (acidic) and 9.0 (alkaline) pH. In addition, the EO release kinetic from the system was investigated in physiological conditions of normal (pH= 7.4, Temperature 37°C) and cancerous cells (pH= 5.4, temperature 42°C). Various niosomal formulations were prepared using thin-film method and the particle’s size, morphology, zeta potential and chemical interactions were characterized. Then, antioxidant activity of nanoparticles was evaluated by DPPH radical-scavenging assay. Results of the present study showed that the niosomal formulation containing soy phosphatidyl cholin, cholesterol, and tween-60 loaded with mint or Mentha pulegium EO (0.5 mg/mL),hydrated with PBS at pH= 9, had the highest loading efficiency. The EO loading efficiency for mint and Mentha pulegium was 61.36% and 74.31%, respectively. The investigation of morphology, size, and zeta showed particles to be spherical with the size less than 80 nm and anionic surface charge. EO release in acidic condition of cancerous cells and 42°C temperature was more than physiological conditions of normal cells, representing the sensitivity of synthesized formulation to pH and temperature. The FTIR analysis results showed that no interaction occurred between EO and noisome and the loading of EO into the niosomes did not make any changes in chemical nature of EO. In addition, the antioxidant activity of EO was very well preserved during the encapsulation. | ||
| کلیدواژه‌ها [English] | ||
| Niosome, Lamiaceae, antioxidants activity, controlled-release, Cancer, thermo and pH sensitive | ||
| مراجع | ||
|
- Abdelbary, G. and El-gendy, N., 2008. Niosome-encapsulated gentamicin for ophthalmic controlled delivery. AAPS PharmSciTech, 9(3): 740-747. - Ag Seleci, D., Seleci, M., Walter, J.G., Stahl, F. and Scheper, T., 2016. Niosomes as nanoparticular drug carriers: fundamentals and recent applications. Journal of Nanomaterial, 2016: 1-13. - Al-Sahlany, S.T.G., 2016. Effect of Mentha piperita essential oil against Vibrio spp. isolated from local cheeses. Pakistan Journal of Food Sciences, 26(2): 65-71. - Amoabediny, G., Haghiralsadat, F., Naderinezhad, S., Helder, M.N., Akhoundi Kharanaghi, E., Mohammadnejad Arough, J. and Zandieh-Doulabi, B., 2017. Overview of preparation methods of polymeric and lipid-based (niosome, solid lipid, liposome) nanoparticles: a comprehensive review. International Journal of Polymeric Materials and Polymeric Biomaterials, 67(6): 1-18. - Asprea, M., Leto, I., Bergonzi, M.C. and Bilia, A.R., 2017. Thyme essential oil loaded in nanocochleates: Encapsulation efficiency, in vitro release study and antioxidant activity. LWT-Food Science Technology, 77: 497-502. - Bayala, B., Bassole, I.H.N., Scifo, R., Gnoula, C., Morel, L., Lobaccaro, J.M.A. and Simpore, J., 2014. Anticancer activity of essential oils and their chemical components-a review. American Journal of Cancer Research, 4(6): 591-607. - Benabdallah, A., Rahmoune, C., Boumendjel, M., Aissi, O. and Messaoud, C., 2016. Total phenolic content and antioxidant activity of six wild Mentha species (Lamiaceae) from northeast of Algeria. Asian Pacific Journal of Tropical Biomedicine, 6(9): 760-766. - Cristina, S., Trevisan, C., Pereira, A., Menezes, P. and Barbalho, S.M., 2017. Properties of Mentha piperita: A brief review. World Journal of Pharmaceutical and Medical Research, 3(1): 309-313. - Gautam, N., Mantha, A.K. and Mittal, S., 2014. Essential oils and their constituents as anticancer agents: a mechanistic view. BioMed Research International, 2014: 1-23. - Ge, Y. and Ge, M., 2016. Distribution of Melaleuca alternifolia essential oil in liposomes with Tween 80 addition and enhancement of in vitro antimicrobial effect. Journal of Experimental Nanoscience, 11(5): 345-358. - Giampieri, R., Maccaroni, E., Mandolesi, A., Del Prete, M., Andrikou, K., Faloppi, L., Bittoni, A., Bianconi, M., Scarpelli, M. and Bracci, R., 2017. Mismatch repair deficiency may affect clinical outcome through immune response activation in metastatic gastric cancer patients receiving first-line chemotherapy. Gastric Cancer, 20: 156-163. - Haghiralsadat, F., Amoabediny, G., Forouzanfar, T., Akhoundi Kharanaghi, E., Helder, M.N. and Zandieh-doulabi, B., 2017. A comprehensive review of lipid-based nanoparticle preparation methods. International Journal of Polymeric Materials and Polymeric Biomaterials, 67(6): 383-400. - Haghiralsadat, F., Amoabediny, G., Sheikhha, M.S., Forouzanfar, T., Helder, M.N. and Zandieh-doulabi, B., 2016. A novel approach on drug delivery: Investigation of new nano-formulation of liposomal doxorubicin and biological evaluation of entrapped doxorubicin on various osteosarcomas cell lines. Cell Journal, 19: 55-65. - Hess, L.M., Michael, D., Mytelka, D.S., Beyrer, J., Liepa, A.M. and Nicol, S., 2016. Chemotherapy treatment patterns, costs, and outcomes of patients with gastric cancer in the United States: a retrospective analysis of electronic medical record (EMR) and administrative claims data. Gastric Cancer, 19: 607-615. - Huang, S.C., Ng, K.F., Lee, S.E., Chen, K.H., Yeh, T.S. and Chen, T.C., 2016. HER2 testing in paired biopsy and excision specimens of gastric cancer: the reliability of the scoring system and the clinicopathological factors relevant to discordance. Gastric Cancer, 19: 176-182. - Kanakis, C.D., Petrakis, E.A., Kimbaris, A.C., Pappas, C., Tarantilis, P.A. and Polissiou, M.G., 2012. Classification of Greek Mentha pulegium L. (Pennyroyal) samples, according to geographical location by Fourier transform infrared spectroscopy. Phytochemical Analysis, 23: 34-43. - Kataoka, K., Matsumoto, T., Yokoyama, M., Okano, T., Sakurai, Y., Fukushima, S., Okamoto, K. and Kwon, G.S., 2000. Doxorubicin-loaded poly (ethylene glycol)-poly (β-benzyl-l-aspartate) copolymer micelles: their pharmaceutical characteristics and biological significance. Journal of Controlled Release, 64: 143-153. - Koohi Moftakhari Esfahani, M. and Alavi, S.E., 2014. Drug delivery of pegylated nanoliposomal paclitaxel to breast cancer cell line. New Cellular and Molecular Biotechnology Journal, 4: 47-50. - Li, S., Dai, S. and Shah, N.P., 2017. Sulfonation and antioxidative evaluation of polysaccharides from pleurotus mushroom and streptococcus thermophilus bacteria: a review. Comprehensive Reviews in Food Science and Food Safety, 16(2): 282-294. - Li, W., Chen, H., He, Z., Han, C., Liu, S. and Li, Y., 2015. Influence of surfactant and oil composition on the stability and antibacterial activity of eugenol nanoemulsions. LWT-Food Science and Technology, 62: 39-47. - Londonkar, R.L. and Poddar, P.V, 2009. Studies on activity of various extracts of Mentha arvensis Linn. against drug induced gastric ulcer in mammals. World Journal of Gastrointestinal Oncology, 1: 82-88. - Mittal, S., Mittal, A., Sharma, K. and Alam, S., 2013. Proniosomes as a Drug Carrier for Transdermal Delivery of Candesartan Cilexetil. International Journal of Nano Studies & Technology, 2(2): 17-22. - Naderinezhad, S., Amoabediny, G. and Haghiralsadat, F., 2017. Co-delivery of hydrophilic and hydrophobic anticancer drugs using biocompatible pH-sensitive lipid-based nano-carriers for multidrug-resistant cancers. RSC Advances, 7: 30008-30019. - Nieto, G., Huvaere, K. and Skibsted, L.H., 2011. Antioxidant activity of rosemary and thyme by-products and synergism with added antioxidant in a liposome system. European Food Research and Technology, 233: 11-18. - Sanson, C., Schatz, C., Le Meins, J.F., Soum, A., Thévenot, J., Garanger, E. and Lecommandoux, S., 2010. A simple method to achieve high doxorubicin loading in biodegradable polymersomes. Journal of Controlled Release, 147(3): 428-435. - Sharifi-Rad, J., Sureda, A., Tenore, G.C., Daglia, M., Sharifi-Rad, M., Valussi, M., Tundis, R., Sharifi-Rad, M., Loizzo, M.R. and Ademiluyi, A.O., 2017. Biological activities of essential oils: from plant chemoecology to traditional healing systems. Molecules 22: E70. - Sharma, V., Hussain, S., Gupta, M. and Saxena, A.K., 2014. In vitro anticancer activity of extracts of Mentha Spp. against human cancer cells. Indian Journal of Biochemistry & Biophysics Journal, 51(5): 416-419. - Siahpoosh, A. and Amraee, F., 2011. Antioxidant capacity of various extracts of Asteragalus morinus Boiss aerial parts. Journal of Shahid Sadoughi University of Medical Sciences, 19(4): 437-444. - Soliman, E.A., El-Moghazy, A.Y., El-Din, M.S.M. and Massoud, M.A., 2013. Microencapsulation of essential oils within alginate: formulation and in vitro evaluation of antifungal activity. Journal of Encapsulation and Adsorption Sciences, 3: 48-55. - Trivedi, M., Patil, S., Mishra, R.K. and Jana, S., 2015. Structural and physical properties of biofield treated thymol and menthol. Journal of Molecular Pharmaceutics & Organic Process Research, 3(2): 1-10. - Varona, S., Martín, A. and Cocero, M.J., 2011. Liposomal incorporation of lavandin essential oil by a thin-film hydration method and by particles from gas-saturated solutions. Industrial & Engineering Chemistry Research, 50: 2088-2097. - Weecharangsan, W., Sitthithaworn, W., Siripong, P., Sukma, M., Ngawhirunpat, T., Opanasopit, P., Thani, W., Vallisuta, O., Ruangwises, N. and Kongkathip, B., 2014. Cytotoxic activity of essential oils of Mentha spp. on human carcinoma cells. Journal of Health Research, 28: 9-12. - Yadav, K., Yadav, D., Saroha, K., Nanda, S., Mathur, P. and Syan, N., 2010. Proniosomal Gel: A provesicular approach for transdermal drug delivery. Der Pharmacia Lettre, 2: 189-198. - Yin, M.C. and Faustman, C., 1993. Influence of temperature, pH, and phospholipid composition upon the stability of myoglobin and phospholipid: A liposome model. Journal of Agricultural and Food Chemistry, 41: 853-857. - Zawadzki, S.F., Perussello, C.A., Müller, P.S. and Scheer, A.P., 2016. Encapsulation efficiency and thermal stability of orange essential oil microencapsulated by spray drying and by coacervation. Boletim do Centro de Pesquisa de Processamento de Alimentos, 34: 133-150. | ||
|
آمار تعداد مشاهده مقاله: 1,147 تعداد دریافت فایل اصل مقاله: 1,344 |
||