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سنتز نانو ذرات بر پایه سورفکتنت حاوی اسانس Mentha piperita L. و Mentha pulegium L. بهمنظور بررسی رهایش اسانس و فعالیت آنتیاکسیدانی | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 2، دوره 34، شماره 3 - شماره پیاپی 89، مرداد و شهریور 1397، صفحه 359-379 اصل مقاله (793.18 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2018.110161.1997 | ||
| نویسندگان | ||
| مطهره السادات مظلوم1؛ سمیرا نادری نژاد* 2؛ فرشته واحدیان1؛ فاطمه حقیرالسادات3؛ فاطمه دانشمند4 | ||
| 1دانشجوی کارشناسی ارشد، گروه بیوشیمی، دانشگاه پیام نور تفت، یزد، ایران | ||
| 2کارشناسارشد مهندسی بیوتکنولوژی، گروه مهندسی بیوتکنولوژی و داروسازی، دانشکده مهندسی شیمی، پردیس دانشکدههای فنی، دانشگاه تهران، تهران، ایران | ||
| 3دکترای نانوبیوتکنولوژی، گروه مهندسی علوم زیستی، دانشکده علوم و فنون نوین، دانشگاه تهران، تهران، ایران | ||
| 4استادیار، گروه زیستشناسی، دانشگاه پیام نور، یزد، ایران | ||
| چکیده | ||
| اسانسها ترکیبهایی بسیار فرّار هستند. انکپسوله کردن اسانس درون حامل دارویی باعث کاهش فرّاریت اسانس، کاهش اکسیدشوندگی و افزایش بازدهی اثرگذاری اسانس میشود. هدف از این تحقیق ساخت نانونیوزوم زیست سازگار حاوی اسانس نعناع (Mentha piperita L.) و پونه (Mentha pulegium L.) و توسعه سامانه گیاهی-دارویی با میزان بارگذاری مناسب میباشد. از اینرو، اثر مقدار و نوع سورفکتنت و همچنین مقدار فسفولیپید بر روی فرمولاسیونهای مختلف نیوزومی بررسی شد. در ادامه با بررسی اثر اسیدیته بافر هیدراته کننده در pHهای 4/7 (خنثی)، 5 (اسیدی) و 9 (بازی) فرمولاسیون بهینه از نظر درصد بارگذاری اسانس معرفی شد. همچنین سینتیک رهایش اسانس در بافر PBS و دو pH، 7.4 (سلول سالم) و 4/5 (سلول سرطانی) و همچنین در دو سطح دماهای 37 (شرایط فیزیولوژیک) و 42 (سلول سرطانی) درجه سلسیوس بر رهایش سامانه حاوی اسانس بررسی شد. فرمولاسیونهای مختلف نیوزومی با استفاده از روش هیدراتاسیون فیلم نازک تهیه شد و اندازه ذرات، مورفولوژی، پتانسیل زتا و برهمکنش شیمیایی مشخصهیابی شد و بعد خاصیت آنتیاکسیدانی نانوذرات با روش مهار رادیکال DPPH ارزیابی شد. نتایج این تحقیق نشان داد که فرمولاسیون نیوزومی حاوی فسفاتیدیل کولین سویا، کلسترول و توئین 60 بارگذاری شده با اسانس نعناع یا پونه با غلظت mg/mL5/0، هیدراته شده با PBS در 9=pH بیشترین بازدهی بارگذاری را نشان دادهاست. میزان اسانس بارگذاری شده برای اسانس نعناع و پونه بهترتیب 61.36% و 74.31% میباشد. ذرات بهصورت کروی با اندازه کمتر از 80 نانومتر و آنیونی میباشند. همچنین میزان رهایش اسانس در شرایط اسیدی و دمای 42 درجه سلسیوس بیشتر از شرایط فیزیولوژیک میباشد که نشان میدهد فرمولاسیون تهیه شده حساس به دما و pH میباشد. نتایج حاصل از آنالیز طیف مادون قرمز نشان داده است که بین اسانس و نیوزوم برهمکنش شیمیایی رخ نداده است و بارگذاری اسانس درون نیوزومها بهصورت فیزیکی بوده است. همچنین خاصیت آنتیاکسیدانی اسانس طی درونگیری حفظ شدهاست. | ||
| کلیدواژهها | ||
| نیوزوم؛ نعناعیان؛ فعالیت آنتیاکسیدانی؛ آهسته رهش؛ سرطان؛ حساس به دما و .pH | ||
| عنوان مقاله [English] | ||
| Synthesize of nanoparticle-based surfactants containing Mentha piperita L. and Mentha pulegium L. essential oil in order to study the essential oil release and antioxidant activity | ||
| نویسندگان [English] | ||
| M. Mazloom1؛ S. Naderinezhad2؛ F. Vahedian1؛ F. Haghiralsadat3؛ F. Daneshmand4 | ||
| 1M.Sc. student, Department of Biochemistry, Payame Noor University, Taft, Yazd, Iran | ||
| 2M.Sc Biotechnology, Department of Biotechnology and Pharmaceutical Engineering, School of Engineering, University of Tehran, Tehran, Iran | ||
| 3Ph.D. in Nanobiotechnology, Faculty of New Science and Technologies, University of Tehran, Tehran, Iran | ||
| 4Department of Biology, Payam Nour University, Taft, Yazd, Iran | ||
| چکیده [English] | ||
| Essential Oils (EO) are highly volatile compounds. Encapsulation of EO into the nano-carrier leads to reduced EO volatility and oxidation, as well as increased therapeutic efficiency. The aim of this study was to synthesize a biocompatible nano-niosome containing Mentha piperita L. and Mentha pulegium L. EO and develop herbal-medicine system with suitable encapsulation efficiency. Hence, the amount and type of surfactant and also the amount of phospholipid were investigated in various niosomal formulations. Furthermore, the optimized formulation was introduced in terms of EO loading efficiency by investigating the acidity effect of hydrating buffer at 7.4 (neutral), 5.0 (acidic) and 9.0 (alkaline) pH. In addition, the EO release kinetic from the system was investigated in physiological conditions of normal (pH= 7.4, Temperature 37°C) and cancerous cells (pH= 5.4, temperature 42°C). Various niosomal formulations were prepared using thin-film method and the particle’s size, morphology, zeta potential and chemical interactions were characterized. Then, antioxidant activity of nanoparticles was evaluated by DPPH radical-scavenging assay. Results of the present study showed that the niosomal formulation containing soy phosphatidyl cholin, cholesterol, and tween-60 loaded with mint or Mentha pulegium EO (0.5 mg/mL),hydrated with PBS at pH= 9, had the highest loading efficiency. The EO loading efficiency for mint and Mentha pulegium was 61.36% and 74.31%, respectively. The investigation of morphology, size, and zeta showed particles to be spherical with the size less than 80 nm and anionic surface charge. EO release in acidic condition of cancerous cells and 42°C temperature was more than physiological conditions of normal cells, representing the sensitivity of synthesized formulation to pH and temperature. The FTIR analysis results showed that no interaction occurred between EO and noisome and the loading of EO into the niosomes did not make any changes in chemical nature of EO. In addition, the antioxidant activity of EO was very well preserved during the encapsulation. | ||
| کلیدواژهها [English] | ||
| Niosome, Lamiaceae, antioxidants activity, controlled-release, Cancer, thermo and pH sensitive | ||
| مراجع | ||
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