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مطالعه تاثیر ملاتونین بر رشد اولیه و برخی خصوصیات جوانه‌زنی و فیزیولوژیکی بذر و گیاهچه بادرشبویه (Dracocephalum moldavica L.) در شرایط تنش اسمزی | ||
| علوم و فناوری بذر ایران | ||
| مقاله 3، دوره 7، شماره 1 - شماره پیاپی 13، مرداد 1397، صفحه 25-40 اصل مقاله (1.92 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2018.108351.1027 | ||
| نویسندگان | ||
| رزیتا کبیری1؛ علی حاتمی* 2؛ حکیمه علومی3؛ مهدی نقی زاده4؛ فاطمه نصیبی5؛ زهرا طهماسبی2 | ||
| 1دانشجوی دکتری دانشگاه ایلام | ||
| 2استادیار دانشگاه ایلام | ||
| 3استادیار دانشگاه دانشگاه تحصیلات تکمیلی صنعتی کرمان | ||
| 4استادیار دانشگاه شهید باهنر کرمان | ||
| 5دانشیار دانشگاه شهید باهنر کرمان | ||
| چکیده | ||
| به منظور بررسی اثر پیشتیمار ملاتونین بر جوانه‌زنی، رشد اولیه و برخی خصوصیات فیزیولوژیکی گیاه دارویی بادرشبویه تحت شرایط تنش اسمزی، آزمایشی بصورت فاکتوریل در قالب طرح کاملاً تصادفی با 25 تیمار و 3 تکرار انجام گرفت. تیمارهای آزمایشی شامل غلظتهای مختلف ملاتونین (µmol 0، 5، 10، 50 و 100) و تنش اسمزی (MPa0، -0.2 0.4، 0.6- و 0.8-) بودند. بذرهای بادرشبویه، به مدت 24 ساعت در غلظت های مختلف ملاتونین پیش تیمار شدند، سپس به منظور انجام آزمون جوانه زنی به پتری دیش‌های حاوی 10 میلی‌لیتر محلول پلی اتیلن گلایکول با غلظتهای ذکر شده منتقل شدند. نتایج نشان داد با افزایش تنش، درصد و سرعت جوانه‌زنی، وزن خشک گیاهچه، شاخص بنیه بذر، طول ریشه چه، وزن خشک ریشه چه، طول اندام هوایی و خشک اندام هوایی، رنگیزه های فتوسنتزی و محتوی پروتئین در بادرشبویه بطور معنی‌داری کاهش و محتوی قندهای محلول افزایش یافت. پیش تیمار بذر با ملاتونین موجب افزایش تحمل گیاه در برابر تنش اسمزی گردید. ملاتونین موجب افزایش درصد و سرعت جوانه‌زنی، شاخص بنیه بذر و در نهایت موجب افزایش وزن خشک گیاهچه های بادرشبویه تحت شرایط تنش گردید. بذرهایی که با غلظتهای بالاتر ملاتونین (µmol 50 و 100) پیشتیمار شده بودند، افزایش صفات فیزیولوژیکی مذکور (رنگیزه های فتوسنتزی، محتوی قندهای محلول و پروتئین) تحت شرایط تنش نسبت به شاهد مشاهده گردید. در تنش اسمزی 0.6- و 0.8- مگاپاسکال، بذرهایی که با آب مقطر پیشتیمار شده بودند، هیچگونه جوانه‌زنی نداشتند، در حالیکه بذرهایی که با غلظتهای 50 و 100 میکرومولار ملاتونین پیش تیمار شده بودند جوانه‌زنی در تمامی سطوح تنش مشاهده گردید. | ||
| کلیدواژه‌ها | ||
| بادرشبویه؛ تنش اسمزی؛ جوانه‌زنی بذر؛ رنگیزه های فتوسنتزی؛ ملاتونین | ||
| عنوان مقاله [English] | ||
| Study the Effect of Melatonin on Early Growth and Some Physiological and Germination Characteristics of Seed and Moldavian Balm (Dracocephalum moldavica) Seedling under Osmotic Stress | ||
| نویسندگان [English] | ||
| Rozita Kabiri1؛ Ali Hatami2؛ Hakimeh Oloumi3؛ Mehdi Naghizadeh4؛ Fatemeh Nasibi5؛ Zahra Tahmasebi2 | ||
| 1PhD Student, Ilam University | ||
| 3Assistant Professor, Graduate University of Advanced Technology | ||
| 4Assistant Professor, Shahid Bahonar University of Kerman | ||
| 5Associate Professor, Shahid Bahonar University of Kerman | ||
| چکیده [English] | ||
| To evaluate the effect of melatonin pretreatment on germination, early seedling growth and some physiological characteristics of Dracocephalum moldavica under osmotic stress, an experiment was conducted in a factorial arrangement based on completely randomized design with 25 treatments and three replications. The experimental treatments were including different concentrations of melatonin (0, 5, 10, 50 and 100 µmol) and osmotic stress (0, -0.2, -0.4, -0.6 and -0.8 MPa). The seeds of moldavian balm which were primed with different concentrations of melatonin for 24 hours, then in order to germination test under osmotic stress, they transferred into sterile petri dishes which contain 10 ml PEG. Results showed that increasing in stress caused reducing of germination percentage and rate, seedling dry weight, seed vigor index, radicle length, radicle dry weight, shoot length, shoot dry weight, photosynthetic pigments, protein content ,increasing and soluble sugar content. Seed priming with melatonin caused an increase of plant tolerance to osmotic stress. Melatonin caused the increment of germination percentage and rate of germination, seed vigor index and ultimately enhanced dry weight of moldavian balm seedlings under stress condition. The seeds which were pretreated with higher concentrations of melatonin (50 and 100 µmol) showed an increasing of physiological characteristics (photosynthetic pigments, soluble sugar content and protein) under stress condition compared to control. No germination was observed in seeds which were pretreated with distilled water at the levels of -0.6 and -0.8 MPa, while the concentrations of 50 and 100 µmol melatonin caused a seed germination at all levels of osmotic stress. | ||
| کلیدواژه‌ها [English] | ||
| moldavian balm, osmotic stress, Seed germination, photosynthetic pigments, Melatonin | ||
| مراجع | ||
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